Isolation, antibiogram and pathogenicity of Salmonella spp. Recovered from slaughtered food animals in Nagpur region of Central India

Abstract

Aim:To determine the prevalence, antibiogram and pathogenicity of Salmonella spp. in the common food animals slaughtered for consumption purpose at government approved slaughter houses located in and around Nagpur region during a period of 2010-2012.Materials and Methods:A total of 400 samples comprising 50 each of blood and meat from each slaughtered male cattle, buffaloes, pigs and goats were collected. Isolation was done by pre-enrichment in buffered peptone water and enrichment in Rappaport-Vassiliadis broth with subsequent selective plating onto xylose lysine deoxycholate agar. Presumptive Salmonella colonies were biochemically confirmed and analyzed for pathogenicity by hemolysin production and Congo red dye binding assay (CRDA). An antibiotic sensitivity test was performed to assess the antibiotic resistance pattern of the isolates.Results:A total of 10 isolates of Salmonella spp. from meat (3 from cattle, 1 from buffaloes and 6 from pigs) with an overall prevalence of 5% among food animals was recorded. No isolation was reported from any blood samples. Pathogenicity assays revealed 100% and 80% positivity for CRDA and hemolytic activity, respectively. Antimicrobial sensitivity test showed multi-drug resistance. The overall resistance of 50% was noted for trimethoprim followed by ampicillin (20%). A maximum sensitivity (80%) was reported to gentamycin followed by 40% each to ampicillin and trimethoprim, 30% to amikacin and 10% to kanamycin.Conclusion:The presence of multidrug resistant and potentially pathogenic Salmonella spp. in slaughtered food animals in Nagpur region can be a matter of concern for public health.

Prevalence and drug susceptibility pattern of Salmonella isolates from apparently healthy slaughter cattle and personnel working at the Jimma municipal abattoir, south-West Ethiopia

“…Reports from Addis Ababa indicated isolation of Salmonella from abdominal 9.8% and from diaphragmatic muscles 11.9% [ 14 ]. The prevalence reported in the current study is higher than other reports such as 0.0% from Egypt [ 15 ], in Namibia 0.50% [ 16 ], 4.5% in Thailand [ 17 ], 6% in Central India [ 18 ], 8.5% in Saudi Arabia [ 19 ] 4.8% [ 9 ], 7.6% [ 20 ] in Baher Dar Ethiopia. This difference possibly arises from the source of animals, types of samples, and sampling technique.…”

Effects of dietary zinc oxide nanoparticles supplementation on growth performance, zinc status, intestinal morphology, microflora population, and immune response in weaned pigs

“…The E. coli was cultured in MaConkey agar (Baisi Biotechnology Co., Ltd, Hangzhou, China) at 37 °C for 24 h . Salmonella was grown in Rappaport–Vassiliadis medium (Baisi Biotechnology Co., LTD. Hangzhou, China) . Bifidobacteria was grown in TPY medium (Baisi Biotechnology Co. Ltd) supplemented at 37 °C .…”

“…26 The E. coli was cultured in MaConkey agar (Baisi Biotechnology Co., Ltd, Hangzhou, China) at 37 ∘ C for 24 h. 27 Salmonella was grown in Rappaport-Vassiliadis medium (Baisi Biotechnology Co., LTD. Hangzhou, China). 28 Bifidobacteria was grown in TPY medium (Baisi Biotechnology Co. Ltd) supplemented at 37 ∘ C. 29 Lactobacillus was cultured in De Man, Rogosa, and Sharpe agar (Baisi Biotechnology Co., Ltd) at 37 ∘ C for 24 h and the plates were incubated in an anaerobic chamber (YQX-II, Yuejin Medical Instrument Co., Ltd, Shanghai, China). 27 Each dilution was counted in triplicate and the microbial enumerations were expressed as log 10 colony-forming units (CFU) per gram with the mean of three replicates.…”

Management of Outbreak of Salmonellosis in a Pig Farm Revealed Human Source of Infection in Cameroon

“…To detect the sensitive antibiotics for the treatment of this case, Salmonella isolates were tested against some antibiotics as described by Kalambhe et al [7] with slight adjustment. In brief, diluted overnight colonies were spread on Müller-Hinton agar using sterile swaps.…”