2011
DOI: 10.1007/s12041-011-0115-5
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Isolation by the 5′ anchored PCR technique and characterization of eighteen microsatellite loci in horseshoe crab (Tachypleus gigas)

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Cited by 5 publications
(3 citation statements)
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“…For non-model organisms, microsatellite loci were generally identified by 5̕ anchor PCR (Ling et al, 2013), transcriptome sequence analysis (Ariede et al, 2018;Han et al, 2018), and microsatellite library screening (Bazsalovicsová et al, 2018) and development of microsatellite loci through cross-species amplification of closely related species (Gravley et al, 2018). In the present study, RAD-seq method was used for isolating microsatellite loci in C. aculeata.…”
Section: Discussionmentioning
confidence: 99%
“…For non-model organisms, microsatellite loci were generally identified by 5̕ anchor PCR (Ling et al, 2013), transcriptome sequence analysis (Ariede et al, 2018;Han et al, 2018), and microsatellite library screening (Bazsalovicsová et al, 2018) and development of microsatellite loci through cross-species amplification of closely related species (Gravley et al, 2018). In the present study, RAD-seq method was used for isolating microsatellite loci in C. aculeata.…”
Section: Discussionmentioning
confidence: 99%
“…For non-model organisms, microsatellite loci were generally identi ed by 5 anchor PCR (Ling et al 2013), transcriptome sequence analysis (Ariede et al 2018;Han et al 2018), and microsatellite library screening (Bazsalovicsová et al 2018) and development of microsatellite loci through cross-species ampli cation of closely related species (Gravley et al 2018). In the present study, RAD-seq method was used for isolating microsatellite loci in C. aculeata.…”
Section: Discussionmentioning
confidence: 99%
“…While the EST-based SSRs still suffer from the drawback that gene / genes not expressing at a particular organ / time are not included, high-throughput DNA sequencing requires substantial investment. The 5’-anchored polymerase chain reaction [ 28 , 29 ] is one of the simplest and fast methodsfor developing SSR enriched genomic libraries and subsequent isolation of microsatellites that does not require substantial investment.…”
Section: Introductionmentioning
confidence: 99%