Isolation, Characterisation and Antagonistic Activity of Bacteria Symbionts Hardcoral <i>Pavona</i> sp. Isolated from Panjang Island, Jepara Against Infectious Multi-drug Resistant (MDR) Bacteria

Ayuningrum, Diah; Kristiana, Rhesi; Asagabaldan, Meezan Ardhanu; Sabdono, Agus; Radjasa, Ocky Karna; Nuryadi, Handung; Trianto, Agus

doi:10.1088/1755-1315/55/1/012029

Cited by 11 publications

(12 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Many researchers reported that marine invertebrate associated-bacteria have potential as antibacterial, i.e. from gorgonia (Kristiana et al 2017), sponge (Asagabaldan et al 2017), and hard coral (Ayuningrum et al 2017;Sibero et al 2018). This study suggested that tunicate-associated bacteria are also potential for bioprospecting of bioactive compounds.…”

Section: Identification Of the Tunicatesmentioning

confidence: 80%

“…The family Bacillaceae showed a high percentage of antimicrobial activity against gram-positive and gramnegative bacteria in previous studies (Arunachalam and Appadorai 2013;Ayuningrum et al 2017). Bacilli produce a wide range of natural products, including lipopeptides, polypeptides, macrolactams, fatty acids, polyketides, and isocoumarins, with a series of bioactivities (Mondol et al 2013;Mohan et al 2016).…”

Section: Crude Extract Activitymentioning

confidence: 95%

“…DNA of the bacteria were extracted using a modified saponin method (Ayuningrum et al 2017). The obtained B I O D I V E R S I T A S 20 (4): 956-964, April 2019 958 DNA sample was examined using NanoDrop 2000 spectrophotometer (Thermo Scientific, USA) to get information about the concentration and purity.…”

Section: S Rdna Identificationmentioning

confidence: 99%

“…The bacterial identification was performed using universal gene primer 27F 5'-AGAGTTTGATCMTGGCTCAG-3 and 1492 R 5'-GGTTACCTTGTTACGACTT-3' (Weisburg et al 1991). PCR Reaction was conducted in an Thermal Cycler (BIO-RAD) T100 using optimization consist of initial denaturation at 95 ℃ for 3 minutes, then followed by 30 cycles of denaturation at 95℃ for 1 min each cycle, annealing at 53.9℃ for 1 minutes, extension at 72℃ for 1 minutes, and followed by final extension at 72℃ for 7 minutes (Ayuningrum et al 2017). PCR products were examined using agarose 1% gel electrophoresis, and the result was visualized by using UVIDoc HD5 (UVITEC Cambridge, UK).…”

Section: S Rdna Identificationmentioning

confidence: 99%

See 3 more Smart Citations

Bacteria associated with tunicate, Polycarpa aurata, from Lease Sea, Maluku, Indonesia exhibiting anti-multidrug resistant bacteria

et al. 2019

View full text Add to dashboard Cite

Abstract. Ayuningrum D, Kristiana R, Nisa AA, Radjasa SK, Muchlissin SI, Radjasa OK, Sabdono A, Trianto A. 2019. Bacteria associated with tunicate, Polycarpa aurata, from Lease Sea, Maluku, Indonesia exhibiting anti-multidrug resistant bacteria. Biodiversitas 20: 956-964. Tunicate is a rich secondary metabolites producer with various biological activities whether as an original producer or produced by the associated microorganisms. In this study, a total of 11 tunicate specimens were identified as Polycarpa aurata with four color variations based on morphological characteristic and COI gene identification and BLAST analysis. The P. aurata associated-bacteria were isolated and tested for antimicrobial activity against multi-drug resistant (MDR) bacteria. A total of 86 axenic isolates were successfully purified. Furthermore, nine isolates (10.5%) exhibited antibacterial activity on preliminary screening. Nine prospective isolates were fermented in respective medium (Zobell 2216, modified M1 or modified ISP2 media) then extracted using ethyl acetate. The ethyl acetate extracts from liquid fermentation were tested against MDR Escherichia coli, MDR Bacillus cereus, Methicillin-Resistant Staphylococcus aureus (MRSA) and Methicillin-Sensitive and Staphylococcus aureus (MSSA). As a result, seven isolates (8.1%) still retained the activity at the extract concentration 150 µg/disk. Molecular analysis based on 16S rDNA sequencing revealed the most active isolates, TSB 47, TSC 10 and TSB 34 identified as Bacillus tropicus, Vibrio alginolyticus and Virgibacillus massiliensis, with BLAST homology 99%.

show abstract

Section: Identification Of the Tunicatesmentioning

confidence: 80%

Section: Crude Extract Activitymentioning

confidence: 95%

Section: S Rdna Identificationmentioning

confidence: 99%

Section: S Rdna Identificationmentioning

confidence: 99%

See 2 more Smart Citations

Bacteria associated with tunicate, Polycarpa aurata, from Lease Sea, Maluku, Indonesia exhibiting anti-multidrug resistant bacteria

et al. 2019

View full text Add to dashboard Cite

show abstract

“…The bacterial DNA that was used for 16S rRNA gene sequencing was amplified with a polymerase chain reaction (PCR) using the universal primers 27F (5ʹAGAGTTTGATCMTGGCTCAG-3ʹ) and 1492R (5ʹTACGGTTAACCTTG TTACGACTT-3ʹ). The PCR conditions used in this study were in accordance with Ayuningrum et al (2017), namely as follows. Initial denaturation at 95°C for three minutes followed by 30 cycles of denaturation at 95°C for one minute per cycle, annealing at 53.9°C for one minute, extension at 72°C for one minute, and the final extension at 72°C for seven minutes.…”

Section: S Rrna Gene Sequence-based Identification Of Active Bactermentioning

confidence: 99%

Antibacterial potential of nudibranch-associated bacteria from Saparua and Nusa Laut Islands, Indonesia

et al. 2019

View full text Add to dashboard Cite

Kristiana R, Sibero MT, Farisa MY, Ayuningrum D, Dirgantara D, Hanafi M, Radjasa OK, Sabdono A, Trianto A. 2019. Antibacterial potential of nudibranch-associated bacteria from Saparua and Nusa Laut Islands, Indonesia. Biodiversitas 20: 1811-1819. Infections caused by multidrug-resistant bacteria are the international health issue that triggers the urgency of finding new antibacterial agents. The aim of this study was to obtain the nudibranchs-associated bacteria that have bioactivity against multidrug-resistant bacteria. A total of 13 species of nudibranch were identified based on morphological characterization. Overlay methods were used for the screening of the isolates bioactivity against six pathogenic multidrug-resistant bacteria. The Minimum Inhibition Concentration (MIC) of the crude extract was evaluated against Methicillin-resistant Staphylococcus aureus (MRSA), Escherichia coli, Bacillus subtilis, Klebsiella pneumonia, Micrococcus luteus, and Extended Spectrum Beta Lactamases E. coli (ESBL) using MTT method. A total of 145 isolates were obtained which eleven of the isolates showed antibacterial activity against the pathogenic bacteria. The MIC te sts showed that the best activity was isolate SM-S-9-15 and SM-N-3-7. The methanolic extract of isolate SM-S-9-15 active to all of the pathogenic bacteria, while the ethyl acetate extract of the isolate SM-N-3-7 active to the E. coli, B. subtilis, K. pneumonia at the concentration of 500 g/mL. According to 16S ribosomal RNA gene sequence-based identification, all active isolates belong to Virgibacillus marismortui, V. dokdonensis, Bacillus kochii, Vibrio algynoliticus, and Pseudoalteromonas piscicida.

show abstract

Microbial Pigments: A Potential Substitute of Synthetic Colorants in the Food and Healthcare Sectors

Patel,

Maharshi,

Gohil

2024

Microbial Products for Health and Nutrition

View full text Add to dashboard Cite

Isolation, Characterisation and Antagonistic Activity of Bacteria Symbionts Hardcoral Pavona sp. Isolated from Panjang Island, Jepara Against Infectious Multi-drug Resistant (MDR) Bacteria

Cited by 11 publications

References 19 publications

Bacteria associated with tunicate, Polycarpa aurata, from Lease Sea, Maluku, Indonesia exhibiting anti-multidrug resistant bacteria

Bacteria associated with tunicate, Polycarpa aurata, from Lease Sea, Maluku, Indonesia exhibiting anti-multidrug resistant bacteria

Antibacterial potential of nudibranch-associated bacteria from Saparua and Nusa Laut Islands, Indonesia

Microbial Pigments: A Potential Substitute of Synthetic Colorants in the Food and Healthcare Sectors

Contact Info

Product

Resources

About