Isolation, Characterisation and Complete Genome Sequence of a Tequatrovirus Phage, Escherichia phage KIT03, Which Simultaneously Infects Escherichia coli O157:H7 and Salmonella enterica

Pham-Khanh, Nguyen Huan; Sunahara, Hirofumi; Yamadeya, Hanako; Sakai, Miho; Nakayama, Tsuneyoshi; Yamamoto, Hiromi; Bich, Van Truong Thi; Miyanaga, Kazuhiko; Kamei, Kaeko

doi:10.1007/s00284-019-01738-0

Cited by 26 publications

(18 citation statements)

References 43 publications

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“…enterica serovars Choleraesuis and Enteritidis) ( 31 , 32 ). In addition, the phylogenetic analysis indicated that Sa45lw contained a long tail fiber gene similar to that of various E. coli O157:H7-specific phages, with the highest evolutionary relationship with Escherichia phage KIT03, able to lyse both E. coli O157:H7 and S. Choleraesuis ( 33 ). On the other hand, Sa45lw harbored a unique gene coding for a tail lysozyme (gp5), which contained a C-terminal domain responsible for recognizing and adsorbing lipopolysaccharide on the host membranes ( 34 ).…”

Section: Discussionmentioning

confidence: 99%

Characterization of a T4-like Bacteriophage vB_EcoM-Sa45lw as a Potential Biocontrol Agent for Shiga Toxin-Producing Escherichia coli O45 Contaminated on Mung Bean Seeds

et al. 2022

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Section: Discussionmentioning

confidence: 99%

Characterization of a T4-like Bacteriophage vB_EcoM-Sa45lw as a Potential Biocontrol Agent for Shiga Toxin-Producing Escherichia coli O45 Contaminated on Mung Bean Seeds

et al. 2022

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“…Generally, bacteriophages show strong host specificity and usually display a species-limited host range. To our knowledge, few phages infecting more than one species of bacteria have been reported (Park et al, 2012;Amarillas et al, 2016;Pham-Khanh et al, 2019). In this study, phage swi3 was isolated from E. coli k88, but the phylogenetic tree analysis results based on the whole-genome sequence and the terminase large subunit showed that all phages with homology in the database were S. enteritidis phages, and the tail-associated protein and the tail tape measure protein were also S. enteritidis phages, which attracted our attention.…”

Section: Discussionmentioning

confidence: 86%

A Novel Polyvalent Bacteriophage vB_EcoM_swi3 Infects Pathogenic Escherichia coli and Salmonella enteritidis

et al. 2021

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A novel virulent bacteriophage vB_EcoM_swi3 (swi3), isolated from swine feces, lyzed 9% (6/65) of Escherichia coli and isolates 54% (39/72) of Salmonella enteritidis isolates, which were all clinically pathogenic multidrug-resistant strains. Morphological observation showed that phage swi3 belonged to the Myoviridae family with an icosahedral head (80 nm in diameter) and a contractile sheathed tail (120 nm in length). At the optimal multiplicity of infection of 1, the one-step growth analysis of swi3 showed a 25-min latent period with a burst size of 25-plaque-forming units (PFU)/infected cell. Phage swi3 remained stable both at pH 6.0–8.0 and at less than 50°C for at least 1 h. Genomic sequencing and bioinformatics analysis based on genomic sequences and the terminase large subunit showed that phage swi3 was a novel member that was most closely related to Salmonella phages and belonged to the Rosemountvirus genus. Phage swi3 harbored a 52-kb double-stranded DNA genome with 46.02% GC content. Seventy-two potential open reading frames were identified and annotated, only 15 of which had been assigned to functional genes. No gene associated with pathogenicity and virulence was identified. The effects of phage swi3 in treating pathologic E. coli infections in vivo were evaluated using a mouse model. The administration of a single intraperitoneal injection of swi3 (106 PFU) at 2 h after challenge with the E. coli strain (serotype K88) (108 colony-forming units) sufficiently protected all mice without toxic side effects. This finding highlighted that phage swi3 might be used as an effective antibacterial agent to prevent E. coli and S. enteritidis infection.

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“…Broad-host range Salmonella phages have been reported in the literature ( Santos et al, 2011 ; Li, M. et al, 2020 ; Tao et al, 2021 ), but phages infecting bacteria from different genera are comparatively rare. It has been reported that phages SFP10 and KIT03 broke the boundary between Salmonella and Escherichia and infected both Salmonella enterica and E. coli O157:H7 ( Park et al, 2012 ; Pham-Khanh et al, 2019 ). A few studies have reported the phages that can cross the genus boundary between Salmonella and Shigella .…”

Section: Resultsmentioning

confidence: 99%

Bacteriophages Isolated From Turkeys Infecting Diverse Salmonella Serovars

Marchant

Thompson

et al. 2022

Front. Microbiol.

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Salmonella is one of the leading causes of foodborne illnesses worldwide. The rapid emergence of multidrug-resistant Salmonella strains has increased global concern for salmonellosis. Recent studies have shown that bacteriophages (phages) are novel and the most promising antibacterial agents for biocontrol in foods because phages specifically kill target bacteria without affecting other bacteria, do not alter organoleptic properties or nutritional quality of foods, and are safe and environmentally friendly. Due to the vast variation in Salmonella serotypes, large numbers of different and highly virulent Salmonella phages with broad host ranges are needed. This study isolated 14 Salmonella phages from turkey fecal and cecal samples. Six phages (Φ205, Φ206, Φ207, ΦEnt, ΦMont, and Φ13314) were selected for characterization. These phages were from all three families in the Caudovirales order. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) revealed that each phage had a unique structural protein profile. Each phage had a distinct host range. Φ207 and ΦEnt are both siphophages. They shared eight hosts, including seven different Salmonella serovars and one Shigella sonnei strain. These two phages showed different restriction banding patterns generated through EcoRI or HindIII digestion, but shared three bands from EcoRI digestion. ΦEnt displayed the broadest and very unusual host range infecting 11 Salmonella strains from nine serovars and three Shigella strains from two species, and thus was further characterized. The one-step growth curve revealed that ΦEnt had a short latent period (10 min) and relatively large burst size (100 PFU/infected cell). ΦEnt and its host showed better thermal stabilities in tryptic soy broth than in saline at 63 or 72°C. In the model food system (cucumber juice or beef broth), ΦEnt infection [regardless of the multiplicity of infections (MOIs) of 1, 10, and 100] resulted in more than 5-log10 reduction in Salmonella concentration within 4 or 5 h. Such high lytic activity combined with its remarkably broad and unusual host range and good thermal stability suggested that ΦEnt is a novel Salmonella phage with great potential to be used as an effective biocontrol agent against diverse Salmonella serovars in foods.

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Isolation, Characterisation and Complete Genome Sequence of a Tequatrovirus Phage, Escherichia phage KIT03, Which Simultaneously Infects Escherichia coli O157:H7 and Salmonella enterica

Cited by 26 publications

References 43 publications

Characterization of a T4-like Bacteriophage vB_EcoM-Sa45lw as a Potential Biocontrol Agent for Shiga Toxin-Producing Escherichia coli O45 Contaminated on Mung Bean Seeds

Characterization of a T4-like Bacteriophage vB_EcoM-Sa45lw as a Potential Biocontrol Agent for Shiga Toxin-Producing Escherichia coli O45 Contaminated on Mung Bean Seeds

A Novel Polyvalent Bacteriophage vB_EcoM_swi3 Infects Pathogenic Escherichia coli and Salmonella enteritidis

Bacteriophages Isolated From Turkeys Infecting Diverse Salmonella Serovars

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