Isolation, characterization and amplification of simple sequence repeat loci in coffee

Cristancho, Marco; Gaitán, Álvaro

doi:10.12702/1984-7033.v08n04a10

Cited by 5 publications

(5 citation statements)

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“…Moreover, the group maintains a strong collaboration with the Sol Genomics Network (SGN) as the mirror of their site in Colombia. Besides building a very robust platform for research on coffee, Cenicafé has performed such important advances as coffee genomics [3]–[5], coffee genetic diversity studies [6], the understanding of plant resistance to diseases and pests [7], the molecular study of coffee pathogens [8]–[10], and the diversity of Beauveria bassiana [11], a fungus with biological control potential not only for coffee pests, but for a wide spectrum of other plant diseases in Colombia.…”

Section: Researchmentioning

confidence: 99%

Computational Biology in Colombia

et al. 2009

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Section: Researchmentioning

confidence: 99%

Computational Biology in Colombia

et al. 2009

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“…Low polymorphism was also reported by Combes et al (2000), who only identified five polymorphic microsatellite markers among 11 SSR primers tested in C. arabica. Furthermore, Cristancho and Gaitan (2008) also identified five polymorphic markers among 12 SSR markers tested in tetraploid Coffea.…”

Section: Discussionmentioning

confidence: 93%

“…Low polymorphism of molecular markers among progenies of C. arabica is well documented (Lashermes et al, 1999;Bertrand et al, 2001), since this species has a narrow genetic base, is autogamous in nature, tetraploid (Cristancho and Gaitan, 2008), and has a low frequency of microsatellite regions in the genome (Cristancho and Gaitan, 2008;AlMurish et al, 2013). Furthermore, in this case, all progenies originated from the crossing of two genotypes.…”

Section: Discussionmentioning

confidence: 98%

Identification of microsatellite markers in coffee associated with resistance to Meloidogyne exigua

Pereira¹,

Setotaw²,

Santos³

et al. 2016

Genet. Mol. Res.

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“…TEs frequently have multiple transposase binding sites adjacent to their TIRs [ 33 , 39 - 42 ]. In other hAT elements, such as Ac, Tol2 and Tag1 , their respective transposases bind to short sequence repeats [ 31 , 33 , 34 , 43 ].…”

Section: Discussionmentioning

confidence: 99%

DNA binding activities of the Herves transposase from the mosquito Anopheles gambiae

et al. 2011

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BackgroundDetermining the mechanisms by which transposable elements move within a genome increases our understanding of how they can shape genome evolution. Class 2 transposable elements transpose via a 'cut-and-paste' mechanism mediated by a transposase that binds to sites at or near the ends of the transposon. Herves is a member of the hAT superfamily of class 2 transposons and was isolated from Anopheles gambiae, a medically important mosquito species that is the major vector of malaria in sub-Saharan Africa. Herves is transpositionally active and intact copies of it are found in field populations of A gambiae. In this study we report the binding activities of the Herves transposase to the sequences at the ends of the Herves transposon and compare these to other sequences recognized by hAT transposases isolated from other organisms.ResultsWe identified the specific DNA-binding sites of the Herves transposase. Active Herves transposase was purified using an Escherichia coli expression system and bound in a site-specific manner to the subterminal and terminal sequences of the left and right ends of the element, respectively, and also interacted with the right but not the left terminal inverted repeat. We identified a common subterminal DNA-binding motif (CG/AATTCAT) that is critical and sufficient for Herves transposase binding.ConclusionsThe Herves transposase binds specifically to a short motif located at both ends of the transposon but shows differential binding with respect to the left and right terminal inverted repeats. Despite similarities in the overall structures of hAT transposases, the regions to which they bind in their respective transposons differ in sequence ensuring the specificity of these enzymes to their respective transposon. The asymmetry with which the Herves terminal inverted repeats are bound by the transposase may indicate that these differ in their interactions with the enzyme.

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Isolation, characterization and amplification of simple sequence repeat loci in coffee

Abstract: -Simple sequence repeat (microsatellite) loci in coffee were identified in clones isolated

Cited by 5 publications

References 50 publications

Computational Biology in Colombia

Computational Biology in Colombia

Identification of microsatellite markers in coffee associated with resistance to Meloidogyne exigua

DNA binding activities of the Herves transposase from the mosquito Anopheles gambiae

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