Isolation, Characterization, and Antitumor Activity of a Novel Heteroglycan from Cultured Mycelia of Cordyceps sinensis

Mei, Yuxia; Yang, Wei; Zhu, Pei-xin; Peng, Nan; Zhu, Haoyu; Liang, Yunxiang

doi:10.1055/s-0034-1382960

Cited by 28 publications

(5 citation statements)

References 29 publications

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“…Our results showed that some WCCPSs have an effect on colon cancer cells in relatively lower or similar concentrations than have been found in previous studies on different cancer cell lines, including colon cancer [ 61 , 62 , 63 , 64 , 65 , 66 , 67 , 68 ]. For instance, Cheng et al showed that Ginseng PS (GPS) caused inhibition of human colon cancer HT-29 cells at 600 μg/mL [ 69 ], Ma and colleagues provided evidence that PS extracted from hawthorn ( Crataegus .)…”

Section: Discussionsupporting

confidence: 81%

Anti-Cancerous Potential of Polysaccharides Derived from Wheat Cell Culture

et al. 2022

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There is a global need to discover effective anti-cancerous compounds from natural sources. Cultivated wheat cells can be a valuable source of non-toxic or low toxic plant-derived polysaccharides. In this study, we evaluated the anti-cancer ability of seven fractions of wheat cell culture polysaccharides (WCCPSs) in the HCT-116 colon cancer cell line. Almost all (6/7) fractions had an inhibitory effect on the proliferation of colon cancer cells, and two fractions (A-b and A-f) had considerable therapeutic indexes. The WCCPS fractions induced cell cycle arrest in the G1 phase and induced different rates of apoptosis (≤48%). Transmission and scanning electron microscopy revealed that WCCPS fractions caused apoptotic changes in the nucleus and cytoplasm, including damage to mitochondria and external morphological signs of apoptosis. In addition, the WCCPSs induced an increase in the levels of Bax, cytochrome c, and caspases 8 and 3, indicating that cell death progressed through intrinsic and extrinsic pathways of apoptosis. Furthermore, some fractions caused a significant decrease of c-Myc, b-catenin, NFkB2, and HCAM (CD 44) levels, indicating enhanced cell differentiation. Thus, for the first time, our results provide a proof of concept of the anti-cancer capacity of WCCPS fractions in colorectal cancer.

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Section: Discussionsupporting

confidence: 81%

Anti-Cancerous Potential of Polysaccharides Derived from Wheat Cell Culture

et al. 2022

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“…The relative contents of the three polysaccharides are 25.64, 33.33 and 41.03%, respectively. So the average molecular weight of CSP is 1.53 × 10 4 kDa from linear regression of the calibration curve, while that of polysaccharide from cultured mycelia of C. sinensis was 117 kDa (Mei et al 2014). The polysaccharide purified from C. militaris fruit bodies had a molecular weight of 5.94 × 10 3 kDa .…”

Section: Molecular Weightmentioning

confidence: 97%

Characterization and In vitro Antioxidant Activity of a Polysaccharide from C ordyceps sobolifera

Hao³

et al. 2015

Journal of Food Processing and Preservation

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The characteristics and antioxidant activities of Cordyceps sobolifera polysaccharide (CSP) were investigated. Results from high-performance gel filtration chromatography showed that the average molecular weight of CSP was 1.53 × 10 4 kDa, while this polysaccharide was mainly composed of mannose, glucose and galactose in the molar ratio of 1.7:8.9:1 as determined by high-performance liquid chromatography after pre-column derivatization with 1-phenyl-3-methyl-5-pyrazolone. Fourier-transform infrared spectrum of CSP indicated the typical functional groups of polysaccharide. Antioxidant activity results showed that CSP had reducing power (OD700 was 0.87 at 1.5 mg/mL), and it also exhibited scavenging activities on hydroxyl (·OH scavenging rate was 47.6% at 5 mg/mL) and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals (scavenging rate was 55.6% at 1 mg/ mL) in a concentration-dependent manner. These results indicate that CSP has good in vitro antioxidant activity and can potentially be used for functional foods. PRACTICAL APPLICATIONSCordyceps sobolifera is a pricy traditional Chinese herb and it has been used in China for a long time. Polysaccharide extracted from C. sobolifera was characterized, and the antioxidant activities of this polysaccharide were investigated. The result may lay the foundation for the development of antioxidant functional foods from C. sobolifera.

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“…It consisted of (1 → 6)-linked α-D-Glc and α-D-Gal, with minor β-(1 → 4)-D-Xyl and β-(1 → 4)-D-Man residues presumably seated in the side chains with a trace quantity of α-(1 → 3)-L-Rha residue. In the assays of the restrained proliferation of sarcoma 180 cells, polysaccharide 1 showed significant activity and induced apoptosis in a dosage-dependent manner [ 80 ]. A novel polysaccharide CME-1 ( 116 ) was identified from C. sinensis , it was composed of mannose and galactose at a 4:6 molar ratio and its molecular weight was 27.6 kDa.…”

Section: Polysaccharidementioning

confidence: 99%

Metabolites and Their Bioactivities from the Genus Cordyceps

Liu

et al. 2022

Microorganisms

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The Cordyceps genus is a group of ascomycete parasitic fungi, and all known species of this genus are endoparasites; they mainly feed on insects or arthropods and a few feed on other fungi. Fungi of this genus have evolved highly specific and complex mechanisms to escape their host’s immune system and coordinate their life cycle coefficients with those of their hosts for survival and reproduction; this mechanism has led to the production of distinctive metabolites in response to the host’s defenses. Herein, we review approximately 131 metabolites discovered in the genus Cordyceps (including mycelium, fruiting bodies and fungal complexes) in the past 15 years, which can be used as an important source for new drug research and development. We summarize chemical structures, bioactivity and the potential application of these natural metabolites. We have excluded some reports that originally belonged to Cordyceps, but whose taxonomic attribution is no longer the Cordyceps genus. This can and will serve as a resource for drug discovery.

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Isolation, Characterization, and Antitumor Activity of a Novel Heteroglycan from Cultured Mycelia of Cordyceps sinensis

Cited by 28 publications

References 29 publications

Anti-Cancerous Potential of Polysaccharides Derived from Wheat Cell Culture

Anti-Cancerous Potential of Polysaccharides Derived from Wheat Cell Culture

Characterization and In vitro Antioxidant Activity of a Polysaccharide from C ordyceps sobolifera

Metabolites and Their Bioactivities from the Genus Cordyceps

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