2011
DOI: 10.1007/s12010-011-9296-x
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Isolation, Characterization, and Catalytic Properties of a Novel Lipase Which Is Activated in Ionic Liquids and Organic Solvents

Abstract: A novel extracellular lipase with organic solvent tolerance was isolated from a local Pseudomonas species. The lipase gene was cloned and expressed in Escherichia coli as a heterologous host and purified by affinity chromatography. The activity of purified lipase was investigated in the presence of imidazolium-based ionic liquids (ILs) such as EMIM[Cl], BMIM[Cl], and HMIM[Cl]. It has been found that the activity of treated lipase with ILs was higher than untreated control in the hydrolysis reaction. Also, the … Show more

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Cited by 27 publications
(18 citation statements)
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“…5, the recombinant enzyme was also investigated for its lipolytic activity against different 1-alkyl-3-methylimidazolium-based ILs ). In agreement with previously reported studies, ILs had promoting influence on lipase activity [7,8]. Besides, the enzyme activity was improved with the increase in the concentration of the ionic liquid.…”
Section: Organic Solventsupporting
confidence: 92%
See 1 more Smart Citation
“…5, the recombinant enzyme was also investigated for its lipolytic activity against different 1-alkyl-3-methylimidazolium-based ILs ). In agreement with previously reported studies, ILs had promoting influence on lipase activity [7,8]. Besides, the enzyme activity was improved with the increase in the concentration of the ionic liquid.…”
Section: Organic Solventsupporting
confidence: 92%
“…Owing to their unique properties including nonvolatility, thermal stability and ionic conductivity as well as nonflammability, ILs are considered as environmentally friendly green solvents [6]. The stimulatory effects of ILs on reactions involving lipases have been reported in previous studies [7,8]. A thermophilic lipolytic bacterium was previously isolated from Dig Rostam hot mineral spring in Iran.…”
Section: Introductionmentioning
confidence: 99%
“…of the organic solvents and the nature of the enzymes [37,38]. The ability of solvents to increase the solubility of substrates, thus facilitating the reaction or maintaining the active structural conformation of the enzyme, might be the cause of higher lipase activity in exposure to organic solvent [37,39].…”
Section: Discussionmentioning
confidence: 99%
“…The ability of solvents to increase the solubility of substrates, thus facilitating the reaction or maintaining the active structural conformation of the enzyme, might be the cause of higher lipase activity in exposure to organic solvent [37,39]. EPA and DHA are commonly obtained from fish oil as byproducts of processing it [40].…”
Section: Discussionmentioning
confidence: 99%
“…The screening on agar plate with Rhodamine B (Figure 1) presented 12 fungi with halos in both acid (pH 5.5) and alkaline medium (pH 8.0), 10 fungi in acidic medium and 4 fungi in alkaline medium (Figure 2). The detection of halos generated by the enzymatic hydrolysis is an easy and valuable method to make lipase screening and can be done using Rhodamine or other dye as Victoria Blue (HASAN et al, 2009;AKBARI et al, 2011). The fungi grew up better in alkaline medium than in acidic medium, consequently the halos measured needed to have different scales.…”
Section: Screening Of Lipolytic Producersmentioning
confidence: 99%