Isolation, Characterization, and Degradation Performance of the 17β-Estradiol-Degrading Bacterium Novosphingobium sp. E2S

Li, Shunyao; Liu, Juan; Sun, Minxia; Ling, Wanting; Zhu, Xuezhu

doi:10.3390/ijerph14020115

Cited by 25 publications

(14 citation statements)

References 45 publications

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“…A positive correlation existed between cell biomass and degradation efficiency. Our results are in par with the findings of Li et al [ 16 ], which reported the degradation of 17 β -estradiol by strain E2S. Several species of bacteria have been reported to be tolerant to OTC— Acinetobacter , Stenotrophomonas maltophilia, and Aeromonas veronii [ 40 ], and flavobacterium strains [ 37 ] which showed 50% removal of OTC.…”

Section: Discussionsupporting

confidence: 89%

“…At low concentrations, OTC and its metabolites increase the development of antibiotic-resistant bacteria (ARB) and accelerate the development, mobilization, and transmission of antibiotic-resistant genes (ARG) [ 14 , 15 ]. This results in altering the microbial community structure in the environment, consequently affecting human and animal health [ 16 ]. ARGs against pathogens are primarily found in soil, surface water, and ground water [ 17 , 18 ].…”

Section: Introductionmentioning

confidence: 99%

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Oxytetracycline Degrading Potential of Lysinibacillus sp. Strain 3+I Isolated from Poultry Manure

Sudha

Parthasarathi

Prabha

et al. 2022

Evidence-Based Complementary and Alternative Medicine

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Oxytetracycline (OTC) which is a broad-spectrum veterinary tetracycline antibiotic is extensively used in poultry farms as a prophylactic, therapeutic, and growth stimulator. Upon administration, unmetabolized OTC is excreted from the animal body through droppings and accumulated in litter in the poultry industry. This study aimed at investigating the OTC degradation potential of an-OTC tolerant bacterial strain, isolated from poultry manure. The isolated strain’s morphology, biochemical properties, and 16S ribosomal RNA (rRNA) gene sequence confirmed that it belonged to the Lysinibacillus genus. To measure the residual OTC concentration, a high-performance liquid chromatography method was used. OTC degradation rates were 2.579 mg L−1d−1 with Lysinibacillus strain 3+I and 1.149 mg L−1d−1 without Lysinibacillus strain 3+I. In the presence of strain 3+I, the half-life significantly reduced to 2.68 days, compared to 6.03 days without strain 3+I. The strain demonstrated 85% removal with the OTC concentration of 10 μg/ml. The influence of pH, temperature, carbon sources, and nitrogen source, which influence degradation, were also investigated. The optimum condition favouring degradation was pH 6 at a temperature of 30°C. In addition, Lysinibacillus sp. strain 3+I’s ability to degrade OTC in poultry litter offers a promising approach to treat poultry manure and effluent containing OTC, preventing its contamination in the environment.

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Section: Discussionsupporting

confidence: 89%

Section: Introductionmentioning

confidence: 99%

Oxytetracycline Degrading Potential of Lysinibacillus sp. Strain 3+I Isolated from Poultry Manure

Sudha

Parthasarathi

Prabha

et al. 2022

Evidence-Based Complementary and Alternative Medicine

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“…[8]. Nevertheless, there are only few bacteria described so far that can catabolize both E2 and TES [16,17,18,19,20,21,22,23] (Table S1).…”

Section: Introductionmentioning

confidence: 99%

Testosterone Degradative Pathway of Novosphingobium tardaugens

Ibero

Galán

Dı́az

et al. 2019

Genes

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In this work, we have shown that Novosphingobium tardaugens NBRC 16725 (strain ARI-1), a bacterial strain that was isolated due to its capacity to mineralize the estrogenic endocrine compound 17β-estradiol, is also able to mineralize testosterone, the androgenic endocrine compound. Using in silico analysis, we predicted a new putative steroid degradation (SD) gene cluster in strain ARI-1, which resembles genes involved in testosterone degradation in Comamonas testosteroni and other testosterone degrading bacteria like Actinobacteria (like Rhodococcus and Mycobacteria genera) although with significant differences in gene organization. A whole transcriptomic analysis of N. tardaugens revealed that testosterone produces a limited induction of the genes of the SD cluster that show a high basal expression in its absence. The 3β/17β-hydroxysteroid dehydrogenase involved in the first metabolic step of testosterone degradation was identified by using genetic and biochemical approaches. The construction of knockout mutant strains in the genes of the SD cluster together with in silico analyses suggests the existence of gene redundancy in the genome of N. tardaugens. This work will expand the knowledge about the metabolic pathways and biotransformation capabilities of a Gram-negative bacterium that could become a new model system in the bacterial steroid degradation field.

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“…Concentrations of these estrogens in the suspended solids were all below the limit of quantification (5.0 ng L −1 ) of the method. Published papers that reported bacterial degradation of β‐E2 and E1 have been limited to studies involving bacteria from sewage (Lee and Liu, 2001), activated sludge (Li et al, 2017), and co‐culture from livestock and poultry wastes (Li et al, 2018), but not in cow manure only.…”

Section: Resultsmentioning

confidence: 99%

Mineralization and Biotransformation of Estrone in Simulated Poultry Litter and Cow Manure Runoff Water

Guardian

Aga

2019

J of Env Quality

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Abbreviations: 14 C-E1, radiolabeled estrone (4-14 C); a-E2, 17a-estradiol; b-E2, 17b-estradiol; E1, estrone; E1-3S, 17b-estrone-3-sulfate; LC-MS/MS, liquid chromatography-tandem mass spectrometry; LOQ, limit of quantification.• A 7-d study showed mineralization and biotransformation of E1 in poultry litter runoff water. • E1 biotransformed to E1-3S in poultry litter runoff water eventually, and mineralized to CO 2 . • Total mineralization of E1 was observed at an average of 92.2% in poultry litter runoff water. • Concentrations of E1 and other estrogens in the cow manure runoff did not change within the 7-day study.

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Isolation, Characterization, and Degradation Performance of the 17β-Estradiol-Degrading Bacterium Novosphingobium sp. E2S

Cited by 25 publications

References 45 publications

Oxytetracycline Degrading Potential of Lysinibacillus sp. Strain 3+I Isolated from Poultry Manure

Oxytetracycline Degrading Potential of Lysinibacillus sp. Strain 3+I Isolated from Poultry Manure

Testosterone Degradative Pathway of Novosphingobium tardaugens

Mineralization and Biotransformation of Estrone in Simulated Poultry Litter and Cow Manure Runoff Water

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