Isolation, characterization and evaluation of the Pichia pastoris sorbitol dehydrogenase promoter for expression of heterologous proteins

Continuous Cultivation as a Tool Toward the Rational Bioprocess Development With Pichia Pastoris Cell Factory

“…These are mainly related with the increased costs and risk of storing and using methanol, as well as the increased oxygen demand and high heat production derived from methanol metabolization (Heyland et al, 2010 ; Prielhofer et al, 2013 ; Çalik et al, 2015 ). These shortcomings have given ground to dedicate important efforts to identify and to develop methanol-free alternatives, which can be classified in two categories: discovery of alternative efficient promoters for P. pastoris (P GAP , P PGK , P THI11 , P PYK , P SDH ) (Periyasamy et al, 2013 ; Landes et al, 2016 ; Juturu and Wu, 2018 ; Massahi and Çalik, 2018 ; de Macedo Robert et al, 2019 ) and engineering or development of synthetic promoters (Ata et al, 2017 ; Wang et al, 2017 ; Prielhofer et al, 2018 ; Vogl et al, 2018b ).…”

Downregulation by organic nitrogen of AOX1 promoter used for controlled expression of foreign genes in the yeast Pichia pastoris

“…Although addition of CA to the culture medium enhances both the cell growth rate and the final biomass, it significantly lowers the cell capacity to metabolize methanol. This effect was not observed for sorbitol, whose conversion into fructose in the presence of NAD + is catalysed by a sorbitol dehydrogenase constitutively produced within the cell (Periyasamy, Govindappa, Sreenivas, & Sastry, ). From this, we could hypothesise that the effect of CA could be more gene‐specific than pleotropic (i.e., inhibition of carbon source catabolism).…”

Systematic assessment of Pichia pastoris system for optimized β -galactosidase production

“…Constitutive promoters were therefore applied as alternatives to overcome these problems [17] . While the GAP promoter was the most commonly used constitutive promoter in P. pastoris system [18] , other promoters like TEF1 [19] and SDH [20] were also reported to have promoting strength comparable to GAP. These three promoters were thereby evaluated and compared.…”