Isolation, characterization and evaluation of microsatellite DNA markers in giant freshwater prawn Macrobrachium rosenbergii, from South India

Divu, D; Khushiramani, Rekha; Malathi, S.; Karunasagar, Iddya

doi:10.1016/j.aquaculture.2008.07.023

Cited by 12 publications

(9 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Divu et al . () evaluated two wild populations in India (Kerala & Karnataka) and reported levels of diversity similar to those found in the India‐wild population evaluated in this study.…”

Section: Discussionsupporting

confidence: 71%

“…Microsatellites or simple sequence repeats is one marker system that recently has become extremely popular for a wide variety of genetic applications in aquaculture (Liu & Cordes ). In recent years, a number of GFP specific microsatellite primer sets have been developed from populations in Australia (Chand, de Bruyn & Mather ), India (Divu, Khushiramani, Malathi, Karunasagar & Karunasagar ; Bhat et al . ), Malaysia (Bhassu, Hassan, Yusoff, Jamari, Shapor & Guan ; Bhassu, See, Hassan, Siraj & Tan ) and Thailand (Chareontawee, Poompuang & Na‐Nakorn ).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Genetic diversity of cultured and wild populations of the giant freshwater prawnMacrobrachium rosenbergii(de Man, 1879) based on microsatellite analysis

et al. 2012

View full text Add to dashboard Cite

Freshwater prawn (Macrobrachium rosenbergii) culture in the Western Hemisphere is primarily, if not entirely, derived from 36 individual prawns originally introduced to Hawaii from Malaysia in 1965 and 1966. Little information is available regarding genetic variation within and among cultured prawn stocks worldwide. The goal of the current study was to characterize genetic diversity in various prawn populations with emphasis on those cultured in North America. Five microsatellite loci were screened to estimate genetic diversity in two wild (Myanmar and India-wild) and seven cultured (Hawaii-1, Hawaii-2, India-cultured, Israel, Kentucky, Mississippi and Texas) populations. Average allelic richness ranged from 3.96 (Israel) to 20.45 (Myanmar). Average expected heterozygosity ranged from 0.580 (Israel) to 0.935 (Myanmar). Many of the cultured populations exhibited reduced genetic diversity when compared with the Myanmar and the India-cultured populations. Significant deficiency in heterozygotes was detected in the India-cultured, Mississippi and Kentucky populations (overall F is estimated of 0.053, 0.067 and 0.108 respectively) reflecting moderate levels of inbreeding. Overall estimate of fixation index (F st = 0.1569) revealed moderately high levels of differentiation among the populations. Outcome of this study provide a baseline assessment of genetic diversity in some available strains that will be useful for the development of breeding programmes.

show abstract

Section: Discussionsupporting

confidence: 71%

Section: Introductionmentioning

confidence: 99%

Genetic diversity of cultured and wild populations of the giant freshwater prawnMacrobrachium rosenbergii(de Man, 1879) based on microsatellite analysis

et al. 2012

View full text Add to dashboard Cite

show abstract

“…The average observed heterozygosities of the three groups ranged from 0.48 to 0.56. This range is comparable to those reported for hatchery and wild population samples of freshwater prawns in previous studies (Chareontawee et al, 2007;Divu et al, 2008;Karaket et al, 2011). Table 5 Genetic distance based on Nei's (1978) The coefficient F IS indicates the degree of departure from random mating.…”

Section: Locussupporting

confidence: 65%

“…Totals of 23, 27 and 21 different alleles were found in all of the loci in the BC, OC and SM male groups, respectively, and these findings explain the high allelic diversities and the extents of allelic richness possessed by these three groups. Divu et al (2008) analysed wild M. rosenbergii using six microsatellite marker loci and reported high levels of allelic diversity in the mean numbers of alleles per loci. In contrast, the available information about the genetic variability levels of the wild giant freshwater prawn M. rosenbergii is limited and can be depicted as the numbers of alleles per microsatellite marker locus (Arora and Bhatia, 2004).…”

Section: Locusmentioning

confidence: 99%

“…In recent years, microsatellites have become one of the most widely used molecular markers for genetic studies, such as studies on the genetic diversity of cultured and wild M. rosenbergii stocks in different regions or countries (Divu et al, 2008;Chareontawee et al, 2007;Liu and Cordes, 2004) and genetic analyses of hatcheryproduced and wild Paralichthys olivaceus strains (Sekino and Hara, 2000;Sekino et al, 2003;Shikano, 2005). In this study, microsatellite markers were used because of their abilities to resolve small (Liu and Cordes, 2004).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Genetic variation among different morphotypes of the male freshwater prawn Macrobrachium rosenbergii (De Man)

Banu

Siraj

Christianus

et al. 2015

Aquaculture Reports

View full text Add to dashboard Cite

a b s t r a c tThe genetic differences between the blue claw male (BC), orange claw male (OC) and small male (SM) morphotypes of male freshwater prawns were assessed using microsatellite DNA markers. Thirty individuals from each of the three groups of male prawns were collected from a hatchery rearing system in Port Dickson, Negeri Sembilan, Malaysia. Significant differences in growth were observed between the three groups. The total numbers of alleles per locus in the BC, OC and SM groups were found to be 23, 27 and 21, respectively from all loci. The average observed heterozygosities (H o ) ranged from 0.48 to 0.56 across all loci in the three groups. The mean F IS of −0.018 indicated an excess of heterozygosity. The pair-wise comparisons and the F ST values revealed significant genetic differentiation across the groups. The greatest genetic distance was observed between the BC and SM groups and lowest one was between the BC and the OC groups.

show abstract

Penaeid Prawns

Hai

Fotedar

Hảo

2011

Recent Advances and New Species in Aquaculture

View full text Add to dashboard Cite

The study focused on the decline in the Marine fishery resources caught by the trawlers. Different Species of the Marine catch like Skates, Rays, Cat fish, Wolf herring, Anchovies, Stolphorous, Thryssa, OtherClupeids, Flying-fish, other perches, Gastropods and Big Pamfrets declined. The decline in catch rate is more for the species like Halibut followed by Stomatopods and Silver bellies. High valued species like.05) showed a declining trend which affects the economic sustainability of the trawler fishery in Kerala. The most prominent centre of the trawl catch in Kerala namely the Neendakara-Shakthikulangara marked a considerable decline in the varieties of the Marine resources caught as a comparison is made between the years 2002 and 2011.

show abstract

Isolation, characterization and evaluation of microsatellite DNA markers in giant freshwater prawn Macrobrachium rosenbergii, from South India

Cited by 12 publications

References 27 publications

Genetic diversity of cultured and wild populations of the giant freshwater prawnMacrobrachium rosenbergii(de Man, 1879) based on microsatellite analysis

Genetic diversity of cultured and wild populations of the giant freshwater prawnMacrobrachium rosenbergii(de Man, 1879) based on microsatellite analysis

Genetic variation among different morphotypes of the male freshwater prawn Macrobrachium rosenbergii (De Man)

Penaeid Prawns

Contact Info

Product

Resources

About