Isolation, characterization and identification of pesticide degrading bacteria from contaminated soil for bioremediation

Mehta, Akshita; Bhardwaj, Kamal Kumar; Shaiza, Mayorin; Gupta, Reena

doi:10.1007/s42977-021-00080-6

Cited by 18 publications

(7 citation statements)

References 12 publications

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“…The result indicated that the growth of bacteria increased by 83.3% from day 3 to day 15 for THIA 1. This indicated that THIA supported the growth of bacterial isolate as the growth was higher than the control (Mehta et al, 2021). Based on Figure 4 (c), the pH value of the enrichment media showed a gradual decline from 6.8 to 6.5 for isolate THIA 1 from day 0 to day 30.…”

Section: Identification Of Thiamethoxam Degradersmentioning

confidence: 82%

Preliminary study on thiamethoxam degrading bacteria isolated from corn plantation

Vigneswari¹,

Sukarman²,

Ariffin³

2021

MJM

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Aims: Thiamethoxam (THIA) is a pesticide that has been widely used for its effectiveness in controlling and preventing insect pests. However, the use of THIA diffused in soil, surface and groundwater pose severe toxicity to the ecosystem. The hazardous pollution caused by the toxicity of THIA demands for remediation to ensure degradation of THIA into its safe constituent elements. Thus, the aim of this study is to isolate and identify potential THIA degraders for future bioremediation. Methodology and results: Bacteria were isolated from soil sample collected at a corn plantation which utilizes THIA as a source of pesticide. Overall, two bacterial isolates were isolated from the soil sample. The bacterial isolates were screened and identified for their ability to degrade pesticide by culturing in minimal salt media (MSM) supplemented with 50 mg/L THIA. The growth of isolates was observed and analyzed through spectrophotometry analysis, standard plate count method and pH value of culture medium. As a result, isolate THIA 1 had been found to possess the ability to degrade pesticide as it showed a high rate in growth of bacteria compared to its controls. Meanwhile, isolate THIA 2 showed no degrading activities while under treatment as it showed similar rate of growth towards its control. Isolate THIA 1 was identified as Acinetobacter sp. UMTFA THIA 1. Conclusion, significance and impact of study:The isolation and identification of the pesticide degrading bacteria will provide promising source of pesticide degrading enzyme that can be further developed for enzymatic pesticide biodegradation. This will pave the way forward in bioremediation where new effective degradation tools can be developed for pesticide residue which otherwise lead to serious ecological problem.

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Section: Identification Of Thiamethoxam Degradersmentioning

confidence: 82%

Preliminary study on thiamethoxam degrading bacteria isolated from corn plantation

Vigneswari¹,

Sukarman²,

Ariffin³

2021

MJM

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“…In the biodegradation, ( Z )-[(1,3-diphenylhept-1-en-1-yl)oxy]trimethylsilane (C 22 H 30 OSi) was highly reduced, and a newly intermediated peak was formed and eicosane (C 20 H 42 ) at a retention time of 18.799, which was degraded into heneicosane (C 21 H 44 ) at a retention time of 18.792. And finally, the peak disappeared due to the degradation …”

Section: Results and Discussionmentioning

confidence: 99%

Integrated Electrochemical Oxidation and Biodegradation for Remediation of a Neonicotinoid Insecticide Pollutant

Satheeshkumar,

Duraimurugan,

Parthipan

et al. 2024

ACS Omega

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A novel integrated electrochemical oxidation (EO) and bacterial degradation (BD) technique was employed for the remediation of the chloropyridinyl and chlorothiazolyl classes of neonicotinoid (NEO) insecticides in the environment. Imidacloprid (IM), clothianidin (CL), acetamiprid (AC), and thiamethoxam (TH) were chosen as the target NEOs. Pseudomonas oleovorans SA2, identified through 16S rRNA gene analysis, exhibited the potential for BD. In EO, for the selected NEOs, the total percentage of chemical oxygen demand (COD) was noted in a range of 58−69%, respectively. Subsequently, in the biodegradation of EO-treated NEOs (BEO) phase, a higher percentage (80%) of total organic carbon removal was achieved. The optimum concentration of NEOs was found to be 200 ppm (62%) for EO, while for BEO, the COD efficiency was increased up to 79%. Fourier-transform infrared spectroscopy confirms that the heterocyclic group and aromatic ring were degraded in the EO and further utilized by SA2. Gas chromatography−mass spectroscopy indicated up to 96% degradation of IM and other NEOs in BD (BEO) compared to that of EO (73%). New intermediate molecules such as silanediamine, 1,1-dimethyl-n,n'-diphenyl produced during the EO process served as carbon sources for bacterial growth and further mineralized. As a result, BEO enhanced the removal of NEOs with a higher efficiency of COD and a lower consumption of energy. The removal efficiency of the NEOs by the integrated approach was achieved in the order of AC > CL > IM > TH. This synergistic EO and BD approach holds promise for the efficient detoxification of NEOs from polluted environments.

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“…(Malghani et al, 2009). Naphade et al (2012) have isolated five bacterial strains from garden soils from Kalyan city, Maharashtra state in India, that tolerated high concentrations of chlorpyrifos, cypermethrin and endosulfan. Of five isolates, two Pseudomonas spp.…”

Section: Rhodococcus Chlorophenolicusmentioning

confidence: 99%

Bioremediation of pesticide-contaminated soil: a review on indispensable role of soil bacteria

et al. 2022

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Purpose: Scientists have identified a plenty of bacterial strains having ability to degrade pesticide residues accumulated in the environment. Due to wide variation of chemical properties of pesticides, a single strain may not be versatile. Hence, identification of bioremediation ability of various bacteria is important. This review focused on understanding and explaining the role of soil bacteria having pesticide detoxification ability.Methodology: Previous research articles, book chapters and literature on bioremediation ability of different soil bacteria were reviewed and various strains of soil bacteria having bioremediation ability, mechanisms behind microbial bioremediation, factors affecting bioremediation and limitations and recent advancements of bioremediation were identified. Based on identified research gaps, further perspectives were introduced. Findings:The results revealed that plenty of soil bacteria having a bioremediation potential have been identified worldwide. As the major mechanism of bioremediation, the microorganisms consume pesticidecontaminated in the soil as their energy or nutrient sources. Various factors such as Bioavailability, Substrate and Environmental factors effect on bioremediation potential. Biostimulation, Bioaugmentation, Biopiling, Composting, Bioreactors and Land farming are identified as popular bioremediation methods suiTable 0for the bioremediation of pesticide contaminants. However, this technique still remains partially developed due to the lack of versatile microorganisms for detoxifying variety of different pesticides.

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Isolation, characterization and identification of pesticide degrading bacteria from contaminated soil for bioremediation

Cited by 18 publications

References 12 publications

Preliminary study on thiamethoxam degrading bacteria isolated from corn plantation

Preliminary study on thiamethoxam degrading bacteria isolated from corn plantation

Integrated Electrochemical Oxidation and Biodegradation for Remediation of a Neonicotinoid Insecticide Pollutant

Bioremediation of pesticide-contaminated soil: a review on indispensable role of soil bacteria

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