Isolation, characterization and optimization of Cystobasidium minutum for phytase production

Abstract

Introduction and Aim: Phytic acid (myo-inositol 1, 2, 3, 4, 5, 6- hexakis dihydro­gen phosphate), a storage form of phosphorus is present in legumes, cereals and oilseeds. By forming insoluble complexes with proteins and divalent cations, it serves as an anti-nutrient in animal feed. Phytase (EC 3.1.3.8), a special class of phosphomonoesterases, catalyse the conversion of inorganic phosphate into mono-, di-, tri-, tetra-, and penta-phosphates of derivatives of myo-inositol. It is an important enzyme in food and feed industries. The present study aimed at isolation, molecular identification and optimization of physico-chemical parameters for phytase production by a red pigmented yeast Cystobasidium minutum.
 
 Materials and Methods: The phytase activity was estimated by using sodium phytate as substrate and the production of phytase under varied temperature, pH, agitation, incubation time, carbon and nitrogen sources.
 
 Results: The results showed maximum activity of 91.86 and 27.13 U/ml at 35°C and pH 5.5. At an agitation speed of 150 rpm and 120h of incubation time the enzyme activity was 18.9 and 21.4 U/ml respectively. Among the carbon sources tested sucrose served for highest enzyme activity of 26.33 U/ml and ammonium sulphate served the sole source of nitrogen and showed an activity of 50.12 U/ml.
 
 Conclusion: Cystobasidium minutum (Rhodotorula minutum) produced maximum phytase enzyme in an optimized physical and chemical condition. Hence, from the present investigation, it is found that optimization of physic-chemical conditions may be a promising tool for the growth of organisms and also maximum yield of any metabolite.

Fermentation: A Potential Strategy for Microbial Metabolite Production

“…The enzymatic digestibility of Monascus purpureus is enhanced by ground maize cob, as previously mentioned, which leads to increased pigment synthesis. Gowthami and Gunashree [92] report optimization of various physico-chemical parameters for the maximum production of phytase by SmF from Cystobasidium minutum, which is imperative for growth of the organism and metabolite yield. Strain improvement of Rhodotorula gracilis by UV mutation and optimization of different media composition of Czapek Dox broth (CDB), Potato Dextrose broth (PDB), Sabaroud's Dextrose broth (SDB), Fat Produing Medium (FPM) for the production of hydrolysate phytase enzyme through SSF and SmF method [93].…”

Yeast Diversity in Honey Produced by Wild Honeybees at Different Elevations

“…The Cystobasidium genus includes mycoparasitic species that are commonly found in various environments, including the phylloplane, soil, freshwater, saltwater, sediments, and regions spanning temperate to cold climates 30 . This species is known not only as a pathogenic species but also as a potential biological control agent for plant diseases and a phytase producer [31][32][33] . While its presence in honey and other food sources is documented, it is important to be aware of the potential for opportunistic infections caused by C. minutum.…”