Isolation, characterization and optimizations of laccase producer from soil: A comprehensive study of application of statistical approach to enhance laccase productivity in Myrothecium verrucaria NFCCI 4363

Jawale, Jayashri. P.; Nandre, Vinod S.; Latpate, Raosaheb; Kulkarni, Mukund G.; Doshi, Pooja

doi:10.1016/j.biteb.2021.100751

Cited by 6 publications

(3 citation statements)

References 39 publications

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“…In the presence of ABTS as a mediator, the laccase discolored acid magenta by about 70% and crystal violet by about 58%. Jawale et al (2021) suggest that the degree of dye decolorization by laccase depends mainly on the origin of the enzyme and the presence of mediators, metal ions, solvents and other compounds in the reaction mixture [198].…”

Section: Synthetic Dyesmentioning

confidence: 99%

Biochemical Characteristics of Laccases and their Practical Application in the Removal of Xenobiotics from Waters

Gałązka¹,

Jankiewicz²,

Szczepkowski³

2023

Preprint

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Industrialization, intensive farming, rapid population growth and urbanization are the source of a large number of pollutants entering the environment. The current concentration of xenobiotics released into the environment exceeds its natural ability to decompose them. Enzymatic degradation of pollutants seems to be an environmentally friendly process. Due to the wide spectrum of substrate specificity, from inorganic compounds to high molecular weight organic compounds such as PAH or dyes, as well as favorable biochemical properties, laccase has been used in the biological removal of xenobiotics from the environment. It is important to understand the degradation mechanisms of pollutants and to evaluate the final products in terms of their toxicity. The laccase oxidizes the substrates with the simultaneous reduction of molecular oxygen to water, which is the purest reaction co-substrate. That is why it is called a green biocatalyst. The trend is an increase in the production of enzymes related to the intensive development of industry, bioremediation or synthetic chemistry. This leads to the search for laccases with greater activity and stability under extreme conditions. The potential of laccases to degrade xenobiotics can be promoted by improving enzymatic catalytic characterization using protein engineering and other genetic engineering methods.

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Section: Synthetic Dyesmentioning

confidence: 99%

Biochemical Characteristics of Laccases and their Practical Application in the Removal of Xenobiotics from Waters

Gałązka¹,

Jankiewicz²,

Szczepkowski³

2023

Preprint

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“…Laccase (EC 1.10.3.2) is a copper rich polyphenol oxidase that catalyzed the oxidation of a wide variety of substrates, which includes several types of phenolic and nonphenolic hazardous compounds (Agrawal et al 2018 ; Jawale et al 2021 ). A major reason for the interest in laccase is its broad range of substrate oxidation activity, the absence of cofactors, and the availability of oxygen as an electron acceptor, which makes them a potentially useful and versatile enzyme with number of biotechnological applications.…”

Section: Introductionmentioning

confidence: 99%

“…The multi-copper laccase is broadly occurring in nature, found in bacteria, fungi, plants, archaea, as well as in insects (Uthandi et al 2010 ). Among these microbial sources, there is a great deal of research being conducted to find novel microbial species exhibiting unique features to use them for laccase production (Jawale et al 2021 ). Moreover, features like small generation time, high proliferation rate, easy to handle and the ability to produce large amount of products using cheap and natural raw materials make them more efficient (Thapa et al 2020 ).…”

Section: Introductionmentioning

confidence: 99%

Optimization of laccase from Stenotrophomonas maltophilia E1 by submerge fermentation using coconut husk with its detoxification and biodecolorization ability of synthetic dyes

Albulaihed,

Adnan,

Jamal

et al. 2023

Bioresour. Bioprocess.

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Enzymatic degradation of synthetic dyes holds an immense promise for addressing the environmental concerns associated with the textile and dye industries. This study aimed to isolate bacteria capable of producing laccase enzymes from an anthropogenic environment. Subsequently, viability of utilizing cost-effective agricultural residues as substrates for laccase production was assessed. Response Surface Methodology (RSM) and the One Variable at a Time (OVAT) approach was pursued for the optimization of laccase production, followed by pH and temperature stability, dye degradation and decolorization experiments, toxicological studies on the degraded dye metabolites. In results, laccase-producing bacterial strain was identified as Stenotrophomonas maltophilia strain E1 (S. maltophilia). Among variety of substrates, coconut husk exhibited optimal efficacy. In a statistical optimization study, it was found that S. maltophilia was capable of producing laccase 51.38 IU/mL, i.e., three times higher than the amount of laccase produced by unoptimized medium (16.7 IU/mL), and the enzyme activity was found to be steady at an acidic pH, and a mesophilic temperature range. The laccase obtained from S. maltophilia E1 demonstrated proficient dye decolorization capabilities, achieving a notable 92.1% reduction in Malachite green dye coloration at a concentration of 500 ppm. Gas chromatography–mass spectrometry (GC–MS) analysis of the decolorized derivatives of Malachite green revealed a conversion into a distinct compounds. Moreover, after undergoing laccase treatment, Malachite green exhibited decreased phytotoxic effects on Oryza sativa, pointing to enzymatic detoxification. Collectively, insights gained from the present study will contribute to the development of efficient enzymatic approaches for addressing the environmental pollution caused by synthetic dyes. Graphical Abstract

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Combinatorial Effect of Copper Ions on the Laccase Productivity of T. versicolor NFCCI 4361 and Its Biomass Application into Spent Wash Remediation

Jawale

Nandre

Kulkarni

et al. 2023

Waste Biomass Valor

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Isolation, characterization and optimizations of laccase producer from soil: A comprehensive study of application of statistical approach to enhance laccase productivity in Myrothecium verrucaria NFCCI 4363

Cited by 6 publications

References 39 publications

Biochemical Characteristics of Laccases and their Practical Application in the Removal of Xenobiotics from Waters

Biochemical Characteristics of Laccases and their Practical Application in the Removal of Xenobiotics from Waters

Optimization of laccase from Stenotrophomonas maltophilia E1 by submerge fermentation using coconut husk with its detoxification and biodecolorization ability of synthetic dyes

Combinatorial Effect of Copper Ions on the Laccase Productivity of T. versicolor NFCCI 4361 and Its Biomass Application into Spent Wash Remediation

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