Isolation, characterization, antimicrobial and other bioactivity profiles of three Streptomyces strains isolated from Lake Gerio, Yola, Adamawa State, Nigeria

Abstract

Background Actinomycetes are prolific producers of bioactive compounds which can be used to combat microbial infections. Microbial infections have resulted to increase in mortality and morbidity rates world-wide, especially in developing nations. This study was carried out to isolate and identify actinomycetes with potentials of synthesizing bioactive compounds, to produce and partially purify antimicrobial extracts, to characterize bioactive compounds in the extracts, to assess their antibacterial activity and to profile other biological properties of the bioactive compounds synthesized by the actinomycetes. Results Streptomyces sp. SUI (MT584797), Streptomyces sp. SW72IV (MT584818) and Streptomyces sp. SW72VII (MT584816) were isolated and identified. Ultraviolet–visible spectra absorption ranged between 241 and 251 nm wavelengths showing the polyene or chromone and unsaturation nature of the natural compounds. Infrared (IR) spectra indicated functional groups such as hydroxyl, aliphatic bromo, carbonyls, esters, carboxylic acids and silicon oxy compounds in the compounds produced by the three strains. Gas Chromatography-Mass Spectrum (GC–MS) identified elaidic acid isopropyl ester (32.11%), Octadec-9-enoic acid (17.44%) and 2, 3-dihydroxyl elaidate (10%) to be mostly produced by Streptomyces sp. SUI, Streptomyces sp. SW72IV and Streptomyces sp. SW72VII respectively. The three strains exhibited antimicrobial activity against Bacillus sp. Pseudomonas aeruginosa ATCC 9077, Staphylococcus aureus ATCC 700699, Candida albicans and Aspergilus flavus. Conclusion The results showed that the three strains of Streptomyces could be sources of antimicrobial bioactive compounds and other secondary metabolites that can be used in the production of pharmaceutical bioactive agents that are effective against pathogens, and production of biological materials that can be used in cosmetics and food industries.

Analysis of Streptomyces Volatilomes Using Global Molecular Networking Reveals the Presence of Metabolites with Diverse Biological Activities

“…The production of some plant-associated metabolites and VOCs in Streptomyces has been observed previously ( 18 , 52 ), highlighting the potential for using bacteria to produce such compounds. In addition, VOCs annotated with high confidence using MSHub/GNPS are reported to display a variety of activities, including antibacterial [1-tridecanol, 3-pentadecanol, and bis(2-ethylhexyl) phthalate] ( 53 – 62 ), antifungal [cis-9-hexadecenal, (Z)-12-octadecenoic acid methyl ester; hexanedioic acid; bis(2-ethylhexyl) ester and octadecyl 3-(3,5-di-tert-butyl-4-hydroxyphenyl) propionate] ( 63 – 69 ), and anticancer [6,10-dimethyl-4-undecanol and 1,2-benzenedicarboxylic acid bis(2-ethylhexyl) ester] ( 70 , 71 ). Bis(2-ethylhexyl) phthalate is often considered a background artifact during the analysis of biologically produced volatiles but has been reported to be produced by many organisms, and there have been some discussions regarding it being classified as a natural product ( 62 ).…”

“…S2). Some of the VOCs annotated using MSHub/GNPS have also been shown to influence insect behavior by acting as pheromones [(6Z,9Z)-6,9-tricosadiene; cis-9-hexadecenal and (Z)-11-hexadecenal] ( 68 , 69 , 72 – 74 ).…”

“…4 ), but the conventional approach annotated some compounds that MSHub/GNPS missed, which included geosmin, cubenol, benzyl alcohol, 1 H -benzocycloheptene, and 4a S -cis-2,4a,5,6,7,8,9,9a-octahydro-3,5,5-trimethyl-9-methylene, all of which have insect repellent activities ( 51 – 54 , 63 , 64 ). In comparison, MSHub/GNPS was able to annotate some VOCs with antibacterial [1-tridecanol, 3-pentadecanol, and bis(2-ethylhexyl) phthalate] ( 53 , 54 ), antifungal [cis-9-hexadecenal, (Z)-12-octadecenoic acid methyl ester, hexanedioic acid, bis(2-ethylhexyl) ester, and octadecyl 3-(3,5-di-tert-butyl-4-hydroxyphenyl)propionate] ( 63 – 69 ), and anticancer [6,10-dimethyl-4-undecanol and 1,2-benzenedicarboxylic acid bis(2-ethylhexyl) ester] ( 70 , 71 ) activities, which were missed by the conventional method. Results suggest that MSHub/GNPS and the conventional method are complementary due to obvious differences in design and workflow, and that the use of both methods is required for thorough volatilome characterization.…”

Phytochemical analysis, radical scavenging and glioblastoma U87 cells toxicity studies of stem bark of buckthorn (Rhamnus pentapomica R. Parker)

“…Another important secondary metabolite, oleic acid, eicosyl ester is well known for its anti-inflammatory, cancer preventive, hypocholesterolaemic, antimicrobial and anemiagenic insectifuge [ 41 ] potentials. The 2, 3 Dihydroxypropyl elaidate also known as monoelaidin has reported to have antimicrobial, antihypertensive, antioxidant and anticancer properties [ 42 ]. Further, 1H-Indene, 2-butyl-5-hexyloctahydro- also has antioxidant, antibacterial, and anti-inflammatory properties and promote wound healing.…”

Isolation and Optimization Growth Parameters for Enhanced Bioactivity Profiles of Streptomyces Strain Isolated from Saudi Arabia

“…It is disseminated in soil, water, and other indigenous habitats (Singh et al, 2006). In all years, Streptomyces strains as a wellspring of various bioactive mixes, for example, anthelminthic chemicals, herbicides, hostile to malignancy drugs, development components, and insusceptible modulators (Kumar et al, 2020;Adeyemo et al, 2021). Factors influencing the number and types of actinomycetes present in a particular soil are geographical location, such as soil type, temperature, organic matter content, moisture content, cultivation, and aeration.…”