Isolation, characterization of Rhodococcus sp. P14 capable of degrading high-molecular-weight polycyclic aromatic hydrocarbons and aliphatic hydrocarbons

Song, Xiaohui; Xu, Yan; Li, Gangmin; Zhang, Ying; Huang, Tongwang; Huang, Zhong

doi:10.1016/j.marpolbul.2011.07.013

Cited by 126 publications

(60 citation statements)

References 24 publications

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“…Among these, microbial degradation is the most thorough, least harmful PAHs removal method (Haritash and Kaushik 2009), which has become a major research direction on pollution remediation. The aerobic degradation of PAHs by microorganisms has been studied (Johnsen et al 2005;Haritash and Kaushik 2009;Wu et al 2009;Song et al 2011;Sun et al 2014) and their biochemical pathway of degradation has been described. But most of the contaminated sites such as sediments and groundwater become anaerobic due to high pollutant concentration and microbial biodegradation processes.…”

Section: Introductionmentioning

confidence: 99%

Anaerobic biodegradation of high-molecular-weight polycyclic aromatic hydrocarbons by a facultative anaerobe Pseudomonas sp. JP1

et al. 2014

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Polycyclic aromatic hydrocarbons (PAHs) are harmful persistent organic pollutants, while the high-molecular-weight (HMW) PAHs are even more detrimental to the environment and human health. However, microbial anaerobic degradation of HMW PAHs has rarely been reported. One facultative anaerobe Pseudomonas sp. JP1 was isolated from Shantou Bay, Shantou, China, which could degrade a variety of HMW PAHs. After 40 days cultivation with strain JP1, anaerobic biodegradation rate of benzo[a]pyrene (BaP), fluoranthene, and phenanthrene was 30, 47, and 5 %, respectively. Consumption of nitrate as the electron acceptor was confirmed by N-(1-naphthyl) ethylenediamine spectrophotometry. Supplementation of sodium sulfite, maltose, or glycine, and in a salinity of 0-20 ‰ significantly stimulated anaerobic degradation of BaP. Lastly, the anaerobic degradation metabolites of BaP by strain JP1 were investigated using GC/MS, and the degradation pathway was proposed. This study is helpful for further studies on the mechanism of anaerobic biodegradation of PAHs.

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Section: Introductionmentioning

confidence: 99%

Anaerobic biodegradation of high-molecular-weight polycyclic aromatic hydrocarbons by a facultative anaerobe Pseudomonas sp. JP1

et al. 2014

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“…Rhodococcus sp. P14 was isolated in Guangdong, China [Song et al, 2011]. Cytochrome P450 monooxygenase from P14 was identified and responsible for oxidization of various PAHs [Luo et al, 2016].…”

Section: Sequence Analysis Of Baa Genesmentioning

confidence: 99%

“…Rhodococcus sp. P14 was isolated from the sediment with crude oil contamination and was able to degrade various PAHs [Song et al, 2011]. In a previous study, cytochrome P450 in Rhodococcus sp.…”

Section: Introductionmentioning

confidence: 99%

Identification of A Ring-Hydroxylating Dioxygenases Capable of Anthracene and Benz[a]anthracene Oxidization from <b><i>Rhodococcus</i></b> sp. P14

et al. 2018

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Nowadays, contamination of soil and marine sediments by polycyclic aromatic hydrocarbons (PAHs) has become a serious problem all over the world. Rhodococcus sp. P14 was isolated from sediments with crude oil contaminate and showed degradation ability on various PAHs. The genome of Rhodococcus sp. P14 was sequenced. A gene cluster encoding a ring-hydroxylating dioxygenase Baa related to PAH degradation was identified by bioinformatics. The expression level of gene baaA was increased when P14 was cultured with anthracene, pyrene, phenanthrene, or benz[a]anthracene as the single carbon source. The recombinant protein Baa was overexpressed in E. coli BL21 (DE3). Further investigations on the recombinant protein Baa in E. coli demonstrated that it was able to oxidize anthracene and benz [a]anthracene, resulting in 9,10-dihydroxyanthracene and 7, 12-dihydroxybenz[a]anthracene as metabolites, respectively. These results indicate that Baa plays an important role in PAH degradation in Rhodococcus sp. P14 and Baa has potential application in the bioremediation of PAHs in the contaminated environment.

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“…Other studies have reported that some microbial species, for instance, Pseudomonas sp., Rhodococcus sp., Mycobacterium sp.,and Alcanivorax sp. could degrade hydrocarbons [20][21][22][23]. .…”

Section: Isolation Of Hydrocarbon Degrading Bacteriamentioning

confidence: 99%

Production of Biosurfactants by Hydrocarbons degrading bacteria isolated from Soummam watershed Sediments of Bejaia in Algeria

Yalaoui-Guellal

Brahmi

Touati

et al. 2017

Env Prog and Sustain Energy

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Hydrocarbons degrading bacteria were isolated from sediments of the Soummam watershed of Bejaia (Algeria). Eleven bacteria strains were isolated using an enrichment technique, method in mineral salt medium, with various hydrocarbons as the sole carbon source. The biodegradation confirmation of various hydrocarbons by these isolates was tested by hole-plate diffusion technique. Out of eleven cultures, nine had shown the growth around the holes. The isolates were screened for biosurfactant producing using oil spreading test and emulsification activity. The value of emulsification activity varied from 55.7 6 1.1 to 78.5 6 0.5%. The diameter of clear zone obtained varied between 20.7 6 1.2 mm and 33.7 6 1.2 mm, it was hence important compared to the negative control. Five bacterial strains were identified as Alcaligenes faecalis, Ochrobactrum, Cellulosimicrobium, Pseudomonas stutzeri, and Rhodococcus ruber by using physiochemical characterization and MALDI-TOF mass spectrometry tools. The best production of crude biosurfactant by the identified bacterial strains was found in R. ruber which produced 6.7 6 0.1 g/L of the crude biosurfactant after 168 h incubation in mineral salt medium (MSM) supplemented with 2% of glucose and 0.1 g/L of yeast extract. The biosurfactant produced by all bacterial strains showed a high emulsification index (E 24 ), where P. stutzeri revealed the highest one (92.2 6 1.1%).

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Isolation, characterization of Rhodococcus sp. P14 capable of degrading high-molecular-weight polycyclic aromatic hydrocarbons and aliphatic hydrocarbons

Cited by 126 publications

References 24 publications

Anaerobic biodegradation of high-molecular-weight polycyclic aromatic hydrocarbons by a facultative anaerobe Pseudomonas sp. JP1

Anaerobic biodegradation of high-molecular-weight polycyclic aromatic hydrocarbons by a facultative anaerobe Pseudomonas sp. JP1

Identification of A Ring-Hydroxylating Dioxygenases Capable of Anthracene and Benz[a]anthracene Oxidization from <b><i>Rhodococcus</i></b> sp. P14

Production of Biosurfactants by Hydrocarbons degrading bacteria isolated from Soummam watershed Sediments of Bejaia in Algeria

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