Isolation, Characterization, Proliferation and Differentiation of Synovial Membrane-derived Mesenchymal Stem Cells (SM-MSCs) from Osteoarthritis Patients

Abstract

<jats:p>Background: Mesenchymal stem cells (MSCs) are the cells which has high renewal capacity and and are capable for differentiating into some types of cells. MSCs can be obtained from several tissues including bone marrow, synovial membrane, blood, adipose tissue and periosteum. The proliferation and self-repair ability of MSCs are the advantages to use as stem cells-based therapy of various diseases. The aim of this study was to determine the differentiation, characterization and priliferation of synovial membrane-derived MSCs (SM-MSCs).Materials and Methods: The cells proliferation capacity was determined by cell counting using trypan blue, characterization of MSCs (cluster of differentiation (CD)90, CD11b, CD73, CD34, CD19, CD45, CD105 and human leukocyte antigen-DR isotype (HLA-DR)) using flow cytometry analysis, and differentiation capability into three lineage cells was determined with red alcian blue, oil red O and alizarin staining.Results: The type culture of SM-MSCs was adherent and showed positive CD44, CD105, CD73, CD90 and negative of CD19, HLA-DR, CD11b, CD45, CD34 surface marker. Based on the result, SM-MSCs P3 showed differentiation potency into adipogenic, chondrogenic, and osteogenic lineage cells. The population doubling time of SM-MSCs has increased from P3 to P8. The population doubling time of SM-MSCs P3 was 1.69 days and SM-MSCs P8 was 3.64 days.Conclusion: The results indicated that SM-MCSCs from osteoarthritis patients are able to differentiate into osteocytes, chondrocytes, adipocytes and highly express of CD105, CD73, CD90, CD44 and negative for CD34, CD45, CD14, CD19.Keywords: synovial membrane, mesenchymal stromal cells, adipocyte, chondrocyte, osteocyte</jats:p>

Conditioned medium of IGF1-induced synovial membrane mesenchymal stem cells increases chondrogenic and chondroprotective markers in chondrocyte inflammation

“…The cells also showed high level of CD90, CD44, CD73, CD105, whilst they showed low expression of hematopoietic lineage markers of CD11b, CD19, CD34, CD45, and HLADR. The MSCs also showed differential capacity into osteogenic, chondrogenic, and adipogenic lineage, which marked their MSCs origins and multipotentiality (26).…”

“…The following experiments were performed after an approval was given by Research Ethics Committee, Faculty of Medicine, Andalas University, Padang, Indonesia (No. 226/KEP/FK/2019) (26).…”

“…The fourth passage of SMMSCs was used for the experiment regarding their population doubling time (PDT) and cumulative population doubling (CPD) as presented in the previous experiment (26). A number of 5 x 10 5 cells in 25 cm 3 culture flask were cultured in Minimum Essential Medium (MEMα) (Biowest, L0475-500) supplemented with 10% Fetal Bovine Serum (FBS) (Biowest, S1810-500) with 1% (v/v) antibiotic-antimycotic (Biowest, L0010-100) and 1% (v/v) nanomycopulitine (Biowest, LX16-010) addition at 37 °C and 5% CO2 condition for 2 days until the cells reached 80% confluency based on previous protocols (24,25,26).…”

3D Biosilica Scaffolds from Melophlus sarasinorum and Xestospongia testudinaria Indonesian Sponges are Biocompatible for Cell Growth and Differentiation of Human Wharton’s Jelly Mesenchymal Stem Cell in Bone Tissue Engineering

“…(8) MSCs are pluripotent stem cells that can be found in a variety of places throughout the body, including bone marrow, fat, vasculature, liver, and umbilical cord blood. (9)(10)(11) Three processes are involved in the formation of bone tissue: osteogenesis, modeling, and remodeling.…”

Characterization of Nanoparticles Conditioned Medium Adipose Tissue Mesenchymal Stem Cell (Cm-Atmsc)

“…According to some evidence, stem cell paracrine signals are involved in the secretion of several growth factors involved in angiogenesis. Therefore, CM treatment can be an alternative therapy [2].…”