Isolation, identification and characterization of lytic, wide host range bacteriophages from waste effluents against Salmonella enterica serovars

Akhtar, Mastura; Viazis, Stelios; Diez-Gonzalez, Francisco

doi:10.1016/j.foodcont.2013.09.064

Cited by 60 publications

(45 citation statements)

References 26 publications

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“…The wastewater samples however, led to isolation of phages belonging to many different genera (but not Jerseyvirus ) with broader host ranges. Wastewater samples are rich in phages infecting other enteric bacteria (Payan et al ., ; Akhtar et al ., ; Mattila et al ., ) and metagenome analysis shows that wastewater contains an immense phage and bacterial diversity (Cantalupo et al ., ). Yet, the survival and propagation of each phage in the wastewater depends on its ability to successfully recognize and infect a host among many bacteria in the tank.…”

Section: Discussionmentioning

confidence: 99%

“…The host range of Salmonella phages was extensively investigated in earlier researches, but not enough complementary data were presented to identify its determinants. Indeed, the host range data was often derived from few strains of relevant serotypes or the phage receptors were not identified (Carey‐Smith et al ., ; Hungaro et al ., ; Moreno Switt et al ., ; Akhtar et al ., ; Wongsuntornpoj et al ., ; Cortes et al ., ). In other cases, Salmonella phage receptors were determined for few phages, but they were not coupled with a comprehensive host range analyses (Ho and Slauch, ; Hong et al ., ; Ricci and Piddock, ; Shin et al ., ).…”

Section: Introductionmentioning

confidence: 99%

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The genera of bacteriophages and their receptors are the major determinants of host range

Gençay

Gambino

Prüssing

et al. 2019

Environmental Microbiology

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Summary The host range of phages is a key to understand their impact on bacterial ecology and evolution. Because of the complexity of phage–host interactions, the variables that determine the breadth of a phage host range remain poorly understood. Here, we propose a novel holistic approach to identify the host range determinants of a new collection of phages infecting Salmonella, isolated from animal, environmental and wastewater samples that were able to infect 58 of the 71 Salmonella strains in our collection. By using a set of statistic approaches (non‐metric dimensional scaling, Bray–Curtis distance, PERMANOVA), we analysed phenotypic (host range on wild‐type and receptor mutants) and genetic data (taxonomic assignment and receptor binding proteins) to evaluate the impact of isolation strain and niche, phage receptor and genus on the host range. Statistical analysis revealed that two phage characteristics influence the host range by explaining the most variance: the receptor by 45% and the genus by 51%. Interestingly, phage genus and receptor in combination explained 79% of the variance, establishing these characteristics as the major determinants of the host range. This study demonstrates the power and the novelty of applying statistical approaches to phenotypic and genetic data to investigate the ecology of phage–host interactions.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The genera of bacteriophages and their receptors are the major determinants of host range

Gençay

Gambino

Prüssing

et al. 2019

Environmental Microbiology

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show abstract

“…According to [10,13]; five sewage samples were collected from broiler farms and centrifuged at low speed (8496 g) for 10 minutes to remove solid particles and then filtered with 0.22 μm pore syringe filters. For bacteriophages enrichment, isolation and purification; S. typhimurium and S. enteritidis were grown overnight at 37°C in nutrient broth to obtain pure bacterial cultures.…”

Section: Methodsmentioning

confidence: 99%

“…Plaque assay was conducted according to Akhtar et al [13], with some modifications; Ten-fold serial dilution was performed using 0.1 ml of the phage suspension (as prepared above). A single colony of overnight culture of S. typhimurium and S. enteritidis was inoculated separately into 5 ml of nutrient broth and incubated at 37°C for 3 hours.…”

Section: Methodsmentioning

confidence: 99%

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Assessing the impact of bacteriophages in the treatment of Salmonella in broiler chickens

Nabil¹,

Tawakol²,

Hassan³

2018

Infection Ecology & Epidemiology

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Salmonellosis is one of the main bacterial infections affecting commercial poultry, causing losses to poultry production, and posing a public health concern.Samples from internal organs (liver, cecum and spleen) of one hundred diseased broiler chickens were collected and subjected to Salmonella isolation, identification and serotyping. S. typhimurium and S. enteritidis were selected from the isolated Salmonella to prepare bacteriophages from sewage water taken at broiler farms. An experimental infection of one day old specific pathogen free (SPF) chicks followed by treatment with the prepared bacteriophages isolated from both Salmonella was performed. Caecal samples from infected chicks were subjected at intervals to bacteriophage isolation and Salmonella quantitation. The effectiveness of bacteriophage treatments on Salmonella colonization in cecum of infected chicks increased after five successive doses. At 3 day post infection (dpi), cecal contents showed a marginal decrease in Salmonella loads with more reduction at 5 dpi. From 7 dpi to the end of the experiment at 15 dpi, all the chicks were cleared for both Salmonella.The findings of this study demonstrate that bacteriophage treatment is efficacious in reducing S. typhimurium and S. enteritidis colonization in broiler chickens within a short period and could be used as an alternative to antibiotics.

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Characterization of SE‐P3, P16, P37, and P47 bacteriophages infecting Salmonella Enteritidis

et al. 2019

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The aim of this study was to identify and characterize the SE-P3, P16, P37, and P47 phages infecting Salmonella Enteritidis. Transmission electron microscopy analysis showed that the SE phages belonged to the Myoviridae or Siphoviridae family and had plaque sizes between 0.622 ± 0.027 and 1.630 ± 0.036 mm in diameter. sefC, pefA, spvC, sopE, and gipA virulent gene regions were absent in their genome and their calculated genome sizes were between 35.9 and 37.8 kbp. Sodium dodecyl sulfate polyacrylamide gel electrophoresis showed that the protein profiles of each phage were different. The SE phages had a short latent period (10-20 min), large burst size (76-356 PFU/cell), and a short burst time (25-35 min). The multiplicity of infection values and mutant frequency of the phages were 0.01-0.0001 and 10 −7 , respectively. They were very infective against their host bacteria when applied at 20°C, 30°C, or 37°C and adsorbed to their host cells by 96.20-97.65% in the first 5 min of incubation, and also Ca 2+ ions did not have a significant effect on their adsorption. The SE phages were resistant to wide pH ranges and high temperatures. These results indicate that the SE phages are good candidates as therapeutic and biocontrol agents against foodborne pathogenic S. Enteritidis.

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Isolation, identification and characterization of lytic, wide host range bacteriophages from waste effluents against Salmonella enterica serovars

Cited by 60 publications

References 26 publications

The genera of bacteriophages and their receptors are the major determinants of host range

The genera of bacteriophages and their receptors are the major determinants of host range

Assessing the impact of bacteriophages in the treatment of Salmonella in broiler chickens

Characterization of SE‐P3, P16, P37, and P47 bacteriophages infecting Salmonella Enteritidis

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