Isolation, Identification, and Characterization of the Novel Antibacterial Agent Methoxyphenyl-Oxime from Streptomyces pratensis QUBC97 Isolate

Sa, Barghouthi; Ayyad, I; Ayesh, Mohammed; Abu‐Lafi, Saleh

doi:10.15744/2574-5980.1.105

Cited by 5 publications

(1 citation statement)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Many important auxins and bioactive metabolites including volatiles, are produced from oximes ( Sørensen et al., 2018 ) and play roles in growth regulation, plant defense and communication. Methoxyphenyl-oxime has been isolated from S. pratensis and had selective antibacterial activity but no effect on S. cerevisiae ( Barghouthi et al., 2017 ). Thus, it is likely to be more beneficial for plant promotion than plant defense against P. noxium .…”

Section: Discussionmentioning

confidence: 99%

In vitro biological control of Pyrrhoderma noxium using volatile compounds produced by termite gut-associated streptomycetes

Adra,

Panchalingam,

Foster

et al. 2024

Front. Plant Sci.

View full text Add to dashboard Cite

IntroductionPyrrhoderma noxium is a plant pathogen that causes economic losses in agricultural and forestry industries, including significant destruction to amenity trees within the city of Brisbane in Australia. Use of chemical control agents are restricted in public areas, there is therefore an urgent need to investigate biological control approaches. Members of the phylum Actinomycetota, commonly known as actinomycetes, are known for their industrially important secondary metabolites including antifungal agents. They have proven to be ideal candidates to produce environmentally friendly compounds including the volatile organic compounds (VOCs) which can be used as biofumigants.MethodsDifferent Streptomyces species (n=15) previously isolated from the guts of termites and stored in the University of the Sunshine Coast’sMicrobial Library were tested for their antifungal VOCs against Pyrrhoderma noxium.ResultsFourteen of them were found to display inhibition (39.39-100%) to the mycelial development of the pathogen. Strongest antifungal activity displaying isolates USC-592, USC-595, USC-6910 and USC-6928 against the pathogen were selected for further investigations. Their VOCs were also found to have plant growth promotional activity observed for Arabidopsis thaliana with an increase of root length (22-36%) and shoot length (26-57%). The chlorophyll content of the test plant had a slight increase of 11.8% as well. Identified VOCs included geosmin, 2-methylisoborneol, 2-methylbutyrate, methylene cyclopentane, β-pinene, dimethyl disulfide, ethyl isovalerate, methoxyphenyl-oxime and α-pinene. Additionally, all 15 Streptomyces isolates were found to produce siderophores and indole acetic acid as well as the enzyme chitinase which is known to break down the fungal cell wall.DiscussionFindings indicate that termite gut-associated streptomycetes might be used to control Pyrrhoderma noxium by utilizing their wide range of inhibitory mechanisms.

show abstract

Section: Discussionmentioning

confidence: 99%