Isolation, identification and in silico analysis of two novel cytoprotective peptides from tilapia skin against oxidative stress-induced ovarian granulosa cell damage

Yin, Haowen; Zhang, Shilin; Hao, Yue; Wang, M; Zeng, Jing; Wu, Wenjin; Wang, Jingfeng; Zheng, Hao; Xue, Chuang; Zhao, Yun-Tao

doi:10.1016/j.jff.2023.105629

Cited by 4 publications

(1 citation statement)

References 42 publications

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“…Yang et al, screen monoamine oxidase A inhibitory peptides utilizing HPEPDOCK and other active peptide screening tools successfully [ 38 ]. Peptides screened via HPEPDOCK exert protective effects on oxidative stress-induced ovarian granulosa cell damage [ 39 ]. In this present study, HPEPDOCK serve was employed to carry out molecular docking assay.…”

Section: Resultsmentioning

confidence: 99%

Isolation and Identification of a Novel Anti-Dry Eye Peptide from Tilapia Skin Peptides Based on In Silico, In Vitro, and In Vivo Approaches

Zeng

Lin

Zhang

et al. 2023

IJMS

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Tilapia skin is a great source of collagen. Here, we aimed to isolate and identify the peptides responsible for combating dry eye disease (DED) in tilapia skin peptides (TSP). In vitro cell DED model was used to screen anti-DED peptides from TSP via Sephadex G-25 chromatography, LC/MS/MS, and in silico methods. The anti-DED activity of the screened peptide was further verified in the mice DED model. TSP was divided into five fractions (TSP-I, TSP-II, TSP-III, TSP-IV, and TSP-V), and TSP-II exerted an effective effect for anti-DED. A total of 131 peptides were identified using LC/MS/MS in TSP-II, and NGGPSGPR (NGG) was screened as a potential anti-DED fragment in TSP-II via in silico methods. In vitro, NGG restored cell viability and inhibited the expression level of Cyclooxygenase-2 (COX-2) protein in Human corneal epithelial cells (HCECs) induced by NaCl. In vivo, NGG increased tear production, decreased tear ferning score, prevented corneal epithelial thinning, alleviated conjunctival goblet cell loss, and inhibited the apoptosis of corneal epithelial cells in DED mice. Overall, NGG, as an anti-DED peptide, was successfully identified from TSP, and it may be devoted to functional food ingredients or medicine for DED.

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Section: Resultsmentioning

confidence: 99%