Isolation, Identification, and Pathogenicity of Two Field Strains of Infectious Bursal Disease Virus

Rosales, Arnaldo; Villegas, Pedro; Lukert, P. D.; Fletcher, O. J.; Mohamed, Mai; Brown, John

doi:10.2307/1591065

Cited by 51 publications

(24 citation statements)

References 18 publications

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“…The rate of isolation of viruses could be higher if SPF eggs were used. The viruses induced 20∼60% mortality within 2∼5 days of PI, with lesions similar to those described earlier (Rosales et al, 1989;Abdel-Alim and Saif, 2001). Abdel-Alim and Saif (2001) reported 100% mortality of SPF embryos within 3∼4 days of PI with very virulent strains of IBDV.…”

Section: Discussionsupporting

confidence: 83%

Isolation and identification of infectious bursal disease virus from broiler and layer chickens during the outbreak year 2007 in Bangladesh

Islam¹,

Mohiuddin²,

Hossain³

et al. 2012

Korean Journal of Veterinary Service

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The objective of the present study was to isolate and identify infectious bursal disease viruses (IBDVs) from broiler and layer chickens of outbreaks of infectious bursal disease (IBD) in three districts of Bangladesh. A total of 70 bursal samples were collected from dead broiler (n=40) and layer (n=30) chickens showing specific lesions of IBD from seven commercial poultry farms of three different districts (Mymensingh, Chittagong and Tangail) of Bangladesh during the year 2007. Five representative bursal samples from each farm were used for the isolation of IBDVs using 9-day-old embryonated eggs of seronegative flock of layer birds and for identification the samples were subjected to agar gel immunodiffusion test (AGIDT), immunohistochemistry (IHC) and reverse transcription-polymerase chain reaction (RT-PCR). Out of 35 bursal samples, IBDVs were successfully isolated from 28 (80%) samples. By AGIDT, 32 (91.4%) samples were found positive for IBDV antigen. Results of AGIDT clearly indicated that IBDVs detected in 29 bursal samples of six affected farms were identical to each other but not to IBDVs present in the remaining three samples of another farm. Indirect immunoperoxidase staining of the bursal sections revealed the presence of IBDV antigen in 32 (91.4%) samples and the IBDV antigen was detected mainly in the cortex of the lymphoid follicles of the bursal tissues. In histopathology, cell depletion, atrophy and necrosis were observed in many bursal follicles with severe edema of interfollicular septa. Of the 35 bursal samples, 34 (97.1%) samples generated 254 bp product by RT-PCR. In conclusion, the results of virus isolation and identification by AGIDT, IHC and the analysis of viral genome by RT-PCR confirmed the outbreaks of acute IBD in commercial poultry of Bangladesh. Moreover, histopathological findings and results of AGIDT gave a clear indication that the isolates from six outbreaks were different from classical strain and it seems to be of very virulent strain. On the other hand, the isolates from the other outbreak were similar to the classical strain.

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Section: Discussionsupporting

confidence: 83%

Isolation and identification of infectious bursal disease virus from broiler and layer chickens during the outbreak year 2007 in Bangladesh

Islam¹,

Mohiuddin²,

Hossain³

et al. 2012

Korean Journal of Veterinary Service

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“…No entanto, o baço foi 22,5% menor para o grupo sob EPC (P<0,07). Nenhum dos órgãos linfóides pesados foi influenciado pelas dietas, resultados que diferem parcialmente dos verificados por Rosales et al (1989) e Donker & Beuving (1989), que encontraram atrofia de todos os órgãos linfóides (timo, baço e bursa de Fabrício) em aves sob EPC. Supondo-se que no calor há maior produção de corticosterona, esperar-se-ia diferença mais consistente no rendimento dos órgãos linfóides.…”

Section: Resultsunclassified

“…A liberação de corticosterona pode ocasionar a involução do tecido linfóide (timo, bursa de Fabrício e baço) e a supressão da imunidade humoral e celular (Rosales et al, 1989).…”

Section: Introductionunclassified

Níveis dietéticos de proteína e gordura e parâmetros bioquímicos, hematológicos e empenamento em frangos de corte estressados pelo calor

Laganá¹,

Ribeiro

González

et al. 2007

R. Bras. Zootec.

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RESUMO -Avaliaram-se dietas com diferentes níveis de PB e gordura e seus efeitos sobre o empenamento e os parâmetros hematológicos e bioquímicos de frangos de corte submetidos a estresse cíclico por calor (EPC) desde os 21 dias ou em ambiente termoneutro (ATN). Foi adotado esquema fatorial 2 x 2 + 2, com dois ambientes -ATN (21-25°C e UR 73%) e EPC (25-32°C e UR 65%) e duas dietas (D) -controle (2,4% gordura e 19,5% proteína) e verão (DV) (4,0% de gordura e 18,5% de proteína com suplementação de Lys e Met + Cys no nível da dieta controle). No ambiente ATN, foram elaboradas outras duas dietas, com quantidade equalizada de ração em relação ao EPC, para ambas as dietas. Foram realizadas análises bioquímicas e hematológicas em seis aves/tratamento, aos 42 dias de idade, e pesados a bursa e baço; em 288 aves foi avaliado o rendimento de penas (RP). A DV para frangos de corte em EPC foi capaz de amenizar parte das alterações observadas no calor, resultando em melhor peso relativo de bursa, menor número de leucócitos totais e menor relação heterófilo/ linfócito. Ambiente e dietas não alteraram os parâmetros bioquímicos séricos. Aves com consumo à vontade no ATN apresentaram RP 15% maior que aves no EPC, embora a restrição alimentar tenha diminuído o RP para as aves com consumo equalizado. As dietas não afetaram o RP. Os dados sugerem que tanto o EPC quanto a restrição alimentar são fatores aditivos em diminuir produção de penas.Palavras-chave: empenamento, estresse por calor, hematologia, gordura, proteína Dietary levels of protein and fat on biochemical and hematological parameters and on feathering in heat stressed broilers ABSTRACT -The objective was to evaluate diets with different levels of CP and fat, and their effect on feathering and hematological and biochemical parameters of broilers submitted to cyclic heat stress (HS) since 21 d of age or in thermoneutral environment (TN). A factorial 2 x 2 + 2 was used, with two environments-TN (21-25°C and RH 73%) and HS (25-32°C and RH 65%), and two diets (D) -control (2.4% fat and 19.5%CP) and summer (SD) (4.0% fat and 18.5% CP with Lys and Met + Cys supplemented at the control diet level). On TN environment, two more diets were formulates, with the amount equalized (pair feed) of ration in relation to HS, for both diet. Biochemistry and hematological analyses were carried out in six birds/ treatment, at 42 days-old, and bursa and spleen weighed and feather yield (FY) was evaluated in 288 birds. The DV for broiler in EPC was capable to attenuate part of the alterations observed in the heat, resulting in better relative weight of bursa, small number of total leukocytes and smaller heterophils/lynphocytes relationship. Environment and diets did not change biochemistry serum parameters. Birds with ad libitum intake in the ATN showed FY 15% greater that birds in the HS, even so the feed restriction decreased RP for the birds with pair feed intake. Diets did not influence FY. Data suggests that either HS or feed intake restriction are additive factors that decrease feather p...

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“…Rosales et al (1989) observaram que aves em EPC apresentaram atrofia de timo, bursa e baço. Puvadolpirod & Thaxton (2000) notaram que o peso relativo de baço reduziu 27%, o de bursa em 43% e o de timo em 65% uma semana após injeção de doses de ACTH em frangos de corte de 5 semanas de idade.…”

Section: Resultsunclassified

Suplementação de vitaminas e minerais orgânicos e sua ação sobre a imunocompetência de frangos de corte submetidos a estresse por calor

et al. 2008

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RESUMO -Estudou-se o efeito de dietas suplementadas com vitaminas C e E e com minerais orgânicos Zn e Se sobre parâmetros imunológicos de frangos de corte de 1 a 35 dias de idade submetidos a estresse cíclico por calor (25 a 32 o C). Avaliou-se ainda a inoculação com albumina sérica bovina (BSA) como estratégia para medir a imunidade humoral. Utilizaram-se 272 aves Ross para avaliação de quatro tipos de suplementação vitamínico-mineral (SVM): D1 -dieta controle com 60 e 30 UI de vit. E/kg de ração nas rações inicial e de crescimento, respectivamente; 0 de vit. C, 80 ppm de Zn inorgânico e 0,3 ppm de Se inorgânico/kg de ração; D2 -dieta controle + 100 UI vit. E e 300 ppm vit. C/kg de ração; D3 -dieta controle + 40 ppm Zn e 0,3 ppm Se orgânicos/kg de ração; D4 -dieta controle + níveis de SVM utilizados em D2 e D3; e de dois ambientes: termoneutro (ATN) e estresse cíclico pelo calor (EPC) a partir dos 14 dias de idade. Seis e cinco aves por tipo de suplementação no EPC e ATN, respectivamente, foram inoculadas com BSA aos 12 e aos 24 dias de idade. O soro das aves com 35 dias de idade foi analisado por ELISA. A suplementação de vitaminas e minerais não influenciou a produção de anticorpos de frangos de corte desafiados com BSA. O estresse por calor provocou maior produção de anticorpos anti-BSA, independentemente da dieta utilizada. A inoculação com BSA não influenciou o desempenho das aves e foi uma estratégia eficiente para avaliar a imunidade humoral de frangos, visto que as aves responderam ao seu desafio com aumento na produção de anticorpos e no tamanho de bursa, apesar da grande variabilidade individual.Palavras-chave: imunomodulação nutricional, selênio, vitamina C, vitamina E, zinco Vitamins and organic minerals supplementation and its effect upon the immunocompetence of broilers submitted to heat stress ABSTRACT -The objective of this experiment was to evaluate the effect of diet supplementation with vitamins C and E and organic minerals Zn and Se on immunological parameters of broilers from 1 to 35 days, kept on cyclic heat stress (25 to 32°C) and to evaluate the usefulness of bovine serum albumin (BSA) inoculation to determine humoral immunity. A total of 272 Ross broilers were used to evaluate four types of vitamin-mineral supplementation (VMS): D1-control diet with 60 and 30 IU of vit E for starter and growing diet, respectively, zero vit C, 80 ppm of inorganic Zn, 0.3 ppm of inorganic Se; D2 -control diet + 100 UI vit E and 300 ppm vit C/kg; D3 -control diet + 40 ppm Zn and 0.3 ppm Se/kg, both from organic sources; D4 -control diet and levels of VMS used in D2 and D3), and two environments: thermoneutral (TNA) and cyclic heat stress (CHS) from 14 days of age. Six and five birds per type of supplementation in CHS and TNA, respectively, were inoculated with BSA at 12 and 24 days. Birds serum at 35 days-old was analyzed by ELISA. Supplementation with vitamins and minerals did not influence antibody production of broilers challenged with BSA. Heat stress improved antibody anti-BSA production regardless of...

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Isolation, Identification, and Pathogenicity of Two Field Strains of Infectious Bursal Disease Virus

Cited by 51 publications

References 18 publications

Isolation and identification of infectious bursal disease virus from broiler and layer chickens during the outbreak year 2007 in Bangladesh

Isolation and identification of infectious bursal disease virus from broiler and layer chickens during the outbreak year 2007 in Bangladesh

Níveis dietéticos de proteína e gordura e parâmetros bioquímicos, hematológicos e empenamento em frangos de corte estressados pelo calor

Suplementação de vitaminas e minerais orgânicos e sua ação sobre a imunocompetência de frangos de corte submetidos a estresse por calor

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