Isolation, identification and pathogenicity study of emerging multi-drug resistant fish pathogen Acinetobacter pittii from diseased rohu (Labeo rohita) in India

Laltlanmawia, C.; Ghosh, Lija; Saha, Ratan K.; Parhi, Janmejay; Pal, Prasenjit; Dhar, Bahni; Saha, Himadri

doi:10.1016/j.aqrep.2023.101629

Cited by 3 publications

(1 citation statement)

References 59 publications

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“…Other Acinetobacter species, such as A. johnsonii, A. lwoffii, A. pittii, A. radioresistens, and A. junii, were found to cause fish disease in recent years, suggesting that Acinetobacter spp. are emerging fish pathogens, posing a new threat to aquaculture [29,49].…”

Section: Bacteriamentioning

confidence: 99%

Microbial Pathogens in Aquaponics Potentially Hazardous for Human Health

Dinev,

Velichkova,

Stoyanova

et al. 2023

Microorganisms

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The union of aquaculture and hydroponics is named aquaponics—a system where microorganisms, fish and plants coexist in a water environment. Bacteria are essential in processes which are fundamental for the functioning and equilibrium of aquaponic systems. Such processes are nitrification, extraction of various macro- and micronutrients from the feed leftovers and feces, etc. However, in aquaponics there are not only beneficial, but also potentially hazardous microorganisms of fish, human, and plant origin. It is important to establish the presence of human pathogens, their way of entering the aforementioned systems, and their control in order to assess the risk to human health when consuming plants and fish grown in aquaponics. Literature analysis shows that aquaponic bacteria and yeasts are mainly pathogenic to fish and humans but rarely to plants, while most of the molds are pathogenic to humans, plants, and fish. Since the various human pathogenic bacteria and fungi found in aquaponics enter the water when proper hygiene practices are not applied and followed, if these requirements are met, aquaponic systems are a good choice for growing healthy fish and plants safe for human consumption. However, many of the aquaponic pathogens are listed in the WHO list of drug-resistant bacteria for which new antibiotics are urgently needed, making disease control by antibiotics a real challenge. Because pathogen control by conventional physical methods, chemical methods, and antibiotic treatment is potentially harmful to humans, fish, plants, and beneficial microorganisms, a biological control with antagonistic microorganisms, phytotherapy, bacteriophage therapy, and nanomedicine are potential alternatives to these methods.

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Section: Bacteriamentioning

confidence: 99%

Microbial Pathogens in Aquaponics Potentially Hazardous for Human Health

Dinev,

Velichkova,

Stoyanova

et al. 2023

Microorganisms

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Genomic insights into fish pathogenic bacteria: A systems biology perspective for sustainable aquaculture

Sasikumar,

Saranya,

Lourdu Lincy

et al. 2024

Fish & Shellfish Immunology

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Novel sequence type of carbapenem-resistant Acinetobacter pittii ST1451 with enhanced virulence isolated from septicaemic neonates in India

Roy,

Morita,

Bhattacharya

et al. 2024

Journal of Antimicrobial Chemotherapy

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Background The clinical relevance of Acinetobacter pittii is increasing, but reports of this organism causing neonatal sepsis are rare. Objectives To understand the mechanisms of resistance and virulence of A. pittii isolated from neonatal blood belonging to a novel sequence type. Materials and methods Antibiotic susceptibility, MLST, WGS, phylogenomic comparison with a global collection of carbapenemase-harbouring A. pittii were done. To study the pathogenic potential of novel A. pittii, in vitro and in vivo assays were carried out. Results and discussion Two novel multidrug-resistant A. pittii from neonatal blood belonging to a novel sequence type 1451 (ST1451) were isolated. WGS revealed that the isolates were almost similar (147 SNP distant) and harbouring two carbapenem resistance genes blaNDM-1 with upstream ISAba125 and downstream bleMBL along with blaOXA-58 with upstream ISAba3. Other resistance genes included blaADC-25, blaOXA-533, aph(3″)-Ib, aph(3′)-VIa, aph(6)-Id, aac(3)-IId, mph(E), msr(E), sul2 and tet(39), different efflux pump genes and amino acid substitutions within GyrA (Ser81Leu) and ParC (Ser84Leu; Glu88Ala) were detected among the isolates. The study genomes were closely related to four strains belonging to ST119. The isolates showed biofilm production, serum resistance, growth under iron limiting condition, surface-associated motility and adherence to host cell. Isolates induced cytokine production in the host cell and showed mice mortality. Discussion and conclusions This study is the first report of the presence of blaNDM-1 in A. pittii from India along with another carbapenemase blaOXA-58. Emergence of highly virulent, multidrug-resistant A. pittii with attributes similar to A. baumannii calls for surveillance to identify the novel strains and their pathogenic and resistance potential.

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Isolation, identification and pathogenicity study of emerging multi-drug resistant fish pathogen Acinetobacter pittii from diseased rohu (Labeo rohita) in India

Cited by 3 publications

References 59 publications

Microbial Pathogens in Aquaponics Potentially Hazardous for Human Health

Microbial Pathogens in Aquaponics Potentially Hazardous for Human Health

Genomic insights into fish pathogenic bacteria: A systems biology perspective for sustainable aquaculture

Novel sequence type of carbapenem-resistant Acinetobacter pittii ST1451 with enhanced virulence isolated from septicaemic neonates in India

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