Isolation, Identification, Characteriterization, And Toxicity Essay Of Non-polar Secondary Metabolite Fraction From Ageratum conyzoides L

Abstract

Ageratum Conyzoides L. has a toxic and bioactive component as anti-cancer and especially for the root, it has anti-tumor. In order to figure out the active component, it is necessary to do four steps; isolation, identification, characterization, and toxicity essay Brine Shrimp Lethality Test (BSLT) of non-polar fraction Ageratum conyzoides L plant. Isolation method consist of four parts; extraction, partitioning of Kupchan method, fractionation, and purification. Fractionation method uses press column chromatography (KTT). Furthermore, purification process uses thin layer chromatography (TLC) and characterization with spectroscopy FTIR, 1H-NMR and 13C-NMR. Then characterization utilizes spectroscopy FTIR, 1H-NMR and 13C-NMR. The toxicity essay toward Artemia Salina Leach n-hexane fraction and dichloromethane fraction showed that the fraction had toxic properties with number of LC50 are 0,0237 and 4,642 µg/mL respectively. Moreover, n-hexane fraction has succeed in isolating Stigmasterol compound shaped white crystalline needles with 19.6 mg weight, 143-144 °C melting point and and had toxic properties with number of LC50 are 30,33 µg/mL.

Antioxidant Activity, Cytotoxicity, and Identification of Secondary Metabolites of Kigelia africana from Waterpark Platinum Riau

“…Toxicity test with BSLT refers to the literature Irmawan et al (2018) and Hanafi et al (2020). Artemia salina eggs were hatched in a container filled with aerated seawater under sufficient light conditions with a 40-60 watt incandescent lamp for 48 hours.…”

“…The BSLT test is a preliminary test used to detect the toxicity of extracts or compounds. The percentage of larval death is proportional to the sample's toxicity, which indicates the biological activity of bioactive ingredients that can be developed as anti-cancer compounds (Irmawan et al, 2018). Sample toxicity is expressed as LC50, the concentration required to kill 50% of the animal population tested.…”