Isolation of a cDNA encoding a 70 kDa heat-shock cognate protein expressed in vegetative tissues ofArabidopsis thaliana

Wu, Shiuh-Hwan; Wang, Chung; Chen, Jychian; Lin, Bai-Ling

doi:10.1007/bf00043887

Cited by 28 publications

(18 citation statements)

References 21 publications

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“…5 and 6). The induction of Hsc70-1 and BiP expression in response to heat shock was in good agreement with previous findings by other laboratories using hybridization-based techniques (Wu et al, 1988(Wu et al, , 1994Koizumi, 1996).…”

Section: Diverse Responses Of Arabidopsis Hsp70s To Temperature Extremessupporting

confidence: 92%

“…Previous expression studies for plant hsp70s demonstrated induction in 2 h of heat shock either at 37°C or 40°C (Wu et al, 1988(Wu et al, , 1994DeRocher and Vierling, 1995;Koizumi, Wu et al, 1996;Li et al, 1999). Most Arabidopsis hsp70s reached peak induction within 30 min of heat shock exposure, and the rapid response of hsp70 genes to heat shock is not limited to Arabidopsis, as the induction of spinach hsp70 genes was detected as early as 5 min after heat shock (Q.…”

Section: Temperature Responsementioning

confidence: 97%

“…At-Hsc70-3/Hsp70-3 mRNA was found to be present at very low levels and showed no induction by heat shock. In addition, At-Hsc70-1/Hsp70-1 was also highly expressed at normal temperature in root, stem, and flower but not detected in green or yellow siliques (Wu et al, 1994).…”

mentioning

confidence: 91%

“…Similar to pea, expression of only three Arabidopsis cytosolic hsp70s has been examined, whereas available sequence information indicates that Arabidopsis has five cytosolic hsp70s, (Wu et al, 1988(Wu et al, , 1994. At-Hsc70-1/Hsp70-1 was shown to be expressed in leaves at normal temperature and further induced by heat shock.…”

mentioning

confidence: 99%

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Comprehensive Expression Profile Analysis of the Arabidopsis Hsp70 Gene Family

2001

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We isolated cDNA clones for two nuclear-encoded, organellar members of the Arabidopsis hsp70 gene family, mtHsc70-2 (AF217458) and cpHsc70-2 (AF217459). Together with the completion of the genome sequence, the hsp70 family in Arabidopsis consists of 14 members unequally distributed among the five chromosomes. To establish detailed expression data of this gene family, a comprehensive reverse transcriptase-polymerase chain reaction analysis for 11 hsp70s was conducted including analysis of organ-specific and developmental expression and expression in response to temperature extremes. All hsp70s showed 2-to 20-fold induction by heat shock treatment except cpHsc70-1 and mtHsc70-1, which were unchanged or repressed. The expression profiles in response to low temperature treatment were more diverse than those evoked by heat shock treatment. Both mitochondrial and all cytosolic members of the family except Hsp70b were strongly induced by low temperature, whereas endoplasmic reticulum and chloroplast members were not induced or were slightly repressed. Developmentally regulated expression of the heat-inducible Hsp70 in mature dry seed and roots in the absence of temperature stress suggests prominent roles in seed maturation and root growth for this member of the hsp70 family. This reverse transcriptase-polymerase chain reaction analysis establishes the complex differential expression pattern for the hsp70s in Arabidopsis that portends specialized functions even among members localized to the same subcellular compartment.

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Section: Diverse Responses Of Arabidopsis Hsp70s To Temperature Extremessupporting

confidence: 92%

Section: Temperature Responsementioning

confidence: 97%

mentioning

confidence: 91%

mentioning

confidence: 99%

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Comprehensive Expression Profile Analysis of the Arabidopsis Hsp70 Gene Family

2001

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“…This may be due to the fact that heat and drought stress often coincide. However, ABA can induce thermo-tolerance in maize and it can activate some genes encoding heat shock proteins (Wu et al 1994;Gong et al 1998). In addition, heat-resistant dwarf mutants can be made sensitive to heat stress by GA treatment (Barnabás et al 2008).…”

Section: Heat Stressmentioning

confidence: 99%

Yield stability for cereals in a changing climate

Powell

Ravash

et al. 2012

Functional Plant Biol.

135

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Abstract. The United Nations Food and Agriculture Organisation (FAO) forecasts a 34% increase in the world population by 2050. As a consequence, the productivity of important staple crops such as cereals needs to be boosted by an estimated 43%. This growth in cereal productivity will need to occur in a world with a changing climate, where more frequent weather extremes will impact on grain productivity. Improving cereal productivity will, therefore, not only be a matter of increasing yield potential of current germplasm, but also of improving yield stability through enhanced tolerance to abiotic stresses. Successful reproductive development in cereals is essential for grain productivity and environmental constraints (drought, cold, frost, heat and waterlogging) that are associated with climate change are likely to have severe effects on yield stability of cereal crops. Currently, genetic gains conferring improved abiotic stress tolerance in cereals is hampered by the lack of reliable screening methods, availability of suitable germplasm and poor knowledge about the physiological and molecular underpinnings of abiotic stress tolerance traits.

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