Isolation of a Highly Active Mineralocorticoid from Beef Adrenal Extract

Grundy, H. M.; Simpson, Sylvia A.; Tait, John

doi:10.1038/169795a0

Cited by 94 publications

(23 citation statements)

References 5 publications

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“…When nearly dry, the extract was transferred to a small tube, and evaporation com¬ pleted under a stream of nitrogen. The assay procedure was that of Simpson & Tait [1952] and is based on changes in the ratio of radioactive sodium to potassium in the urine of adrenalectomized rats. Samples: E, F and is a mixture of cortisone, 17-hydroxycorticosterone and corticosterone respec¬ tively; Aid., 5µg aldosterone; Ext., re-chromatography of the extract from cut 2 of a previous chromatogram of rat adrenal effluent in this system (see text).…”

Section: Animalsmentioning

confidence: 99%

The Secretion of Aldosterone and Corticosterone by the Rat Adrenal

Singer¹,

Stack-Dunne²

1955

Journal of Endocrinology

125

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1. A potent mineralocorticoid has been detected in a paper-chromatographic fraction of extracts of blood from the rat's adrenal vein. The active fraction contains a substance with the same RF and soda-fluorescence reaction as aldosterone, and is present in a concentration corresponding to the observed biological activity.2. Intact rats subject to surgical stress secrete aldosterone (aldosterone-like material) equivalent to about 45 \g=m\gDCA, and 150 \g=m\gcorticosterone/adrenal/kg/hr, with a ratio of 0\m=.\30/1. Treatment with DCA for 7 days or feeding a potassium-deficient diet for 4 weeks reduces aldosterone by more than 90% without altering corticosterone; the ratio is reduced to about 0\m=.\014/1 after induction of the potassium deficiency, and to 0\m=.\044/1after DCA treatment.3. Hypophysectomized rats secrete about 7\m=.\8\g=m\g DCA equivalent of aldosterone and 2 \ g=m\ g corticosterone, giving a ratio of 3\m=.\65/1. In rats 2 days after hypophysectomy, or in rats 7 or 14 days after hypophysectomy and injected daily with corticotrophin, acute stimulation with corticotrophin leads to a greater proportional increase in the secretion of corticosterone than that of aldosterone and results in levels of both comparable with those in intact rats. In the rats 7 days after operation but not maintained on corticotrophin, acute corticotrophin stimulation (see text, p. 134) raises aldosterone to the levels in intact rats, but corticosterone is increased only in about the same proportion as aldosterone and the ratio remains similar to that in untreated hypophysectomized rats.4. It is concluded that the rates of secretion of aldosterone and corticosterone by the rat adrenal vary independently, the ratio depending on the state of the animal. 5. In a single experiment, treatment of hypophysectomized rats with DCA and corticotrophin for 7 days resulted in inhibition of aldosterone secretion analogous to the effect observed in intact rats. This experiment suggests that the action of DCA can occur in the absence of the pituitary. It is possible also that the effect of potassium deficiency on adrenal secretion can occur in the absence of the pituitary.

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Section: Animalsmentioning

confidence: 99%

The Secretion of Aldosterone and Corticosterone by the Rat Adrenal

Singer¹,

Stack-Dunne²

1955

Journal of Endocrinology

125

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show abstract

“…greatly in excess of what could be accounted for by the content of any known adrenal steroid or combination of adrenal steroids (114,38,37). They showed further that the mineralocorticoid activity similar to that in adrenal extracts was also present in adrenal venous blood (99), thus adding great strength to the assumption that a true hormone was involved.…”

Section: The Amorphous Fractionmentioning

confidence: 90%

Aldosterone—a Review*

Gaunt¹,

Renzi²,

Chart³

1955

The Journal of Clinical Endocrinology & Metabolism

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“…As these studies progressed, we were impressed by the similarity of the findings of other investigators (18)(19)(20) on the highly active mineralocorticoid of adrenal cortical extract. This material appears to be very similar, if not identical, with the sodium-retaining corticoid of urine.…”

Section: Discussionmentioning

confidence: 99%

“…This material appears to be very similar, if not identical, with the sodium-retaining corticoid of urine. The only important discrepancy concerned absorption of ultraviolet light near 240 mu (18); this has been resolved by the finding of such absorption in later preparations from adrenal cortical extract (16). These points of similarity between the corticoid from adrenal cortex and from urine, prepared from different sources by different methods, and showing consistent activity during purification, support the idea that both preparations contain a natural hormone secreted by the adrenal cortex and appearing in urine in measurable amounts under the observed conditions.…”

Section: Discussionmentioning

confidence: 99%