Isolation of antibacterials from the mangrove, Avicennia marina and their activity against multi drug resistant Staphylococcus aureus

Dhayanithi, Nagarajan Balachandran; Kumar, T. T. Ajith; Murthy, Ravindra; Kathiresan, K.

doi:10.1016/s2221-1691(12)60516-4

Cited by 26 publications

(16 citation statements)

References 5 publications

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“…The Avicennia marina possess various pharmacological and bioactive activities including anticancer [17][18][19], antimalarial [20][21][22], anti-inflammatory [23][24], antiviral [25][26], antioxidant [27][28][29], antibacterial [20][21][22][23][24][25][26][27][28][29][30][31][32], antifungal [33][34][35], herbal immunostimulant [36] and various protective effects [37] etc., made this mangrove a traceable source for already exist phytochemicals as well as new drug discovery in herbal treatment systems for various disease prevention. Apart from their plentiful uses in several disease treatments and ethnomedicinal worth and practicality, the chromatographies HPTLC pattern analysis or optimized fingerprint of Avicennia marina leaf, stem and root has not been fully reported yet and still very little is known related to whole Phytochemistry of its different plant parts.…”

Section: Introductionmentioning

confidence: 99%

High-Performance Thin Layer Chromatography (Hptlc) Fingerprinting Pattern of Mangrove Avicennia Marina

Dawane

Fulekar

2017

Int J Pharm Pharm Sci

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Objective: An attempt has been made to study phytoconstituents and High-Performance Thin Layer Chromatography (HPTLC) fingerprinting pattern of leaf/stem/root methanol extracts of Avicennia marina. Methods:The phytochemical screening was done by standard biochemical methods and standard optimized HPTLC densitometry determination was performed under two different energy zones [under UV-254 nm and under 540 nm after derivatization at sample size-10 µl, temperature = 25.8±0.3 °C (constant) and relative humidity = 86±1% (constant) in optimized solvent system].Results: Phytochemical analysis confirmed the presence of phytochemicals in the leaf, stem, and root of the plant. Under UV-254 nm energy range, the leaf, stem and root extract showed the presence of 8, 6 and 5 components respectively and under 540 nm energy zone after derivatization, the leaf, stem and root extract revealed the presence of 7, 5 and 5 components, respectively and specific marker components with very high concentration (under UV-254 nm; 3, 2, 2 in leaf, stem, and root respectively and under 540 nm after ASR treatment; 2, 1, 2 in leaf, stem, and root respectively) were detected among them. Conclusion:The preliminary phytochemical test results elucidated Avicennia marina leaf, stem, and root as a hug reservoir of various class of phytochemicals and metabolites. The results obtained by HPTLC fingerprinting method found to be acceptable as a quick, reliable, accurate and economical for identification and authentication of Avicennia marina mangrove plant and useful to differentiate this mangrove species from other similar mangroves, in a single TLC plate run. This serves as a biochemical marker pattern for leaf, stem and root parts of this mangrove and for its diverse phytoconstituents.

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Section: Introductionmentioning

confidence: 99%

High-Performance Thin Layer Chromatography (Hptlc) Fingerprinting Pattern of Mangrove Avicennia Marina

Dawane

Fulekar

2017

Int J Pharm Pharm Sci

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“…marina has been described in the literature for its antibacterial, antifungal, anti-inflammatory and anticancer properties [7,10,18]. The present study was conducted to investigate the anticancer potential of ethyl acetate extract of leaves and stems of AM.…”

Section: Discussionmentioning

confidence: 99%

“…The chemical investigations of the plant have revealed the presence of terpenoids and steroids, naphthalene derivatives, flavones, iridoid glucosides, phenyl propanoid glycosides, flavonoids and abietane diterpenoid glucosides. Biological activities of the different extracts or isolated molecules from the plant species include antimicrobial [7,8,9], anti-inflammatory [10], antiplasmoidal [11], antioxidant [12], antifouling [13] and anticancer effect [6,14,15,16,17,18].…”

Section: Introductionmentioning

confidence: 99%

Autophagy Inhibition Enhances the Mitochondrial-Mediated Apoptosis Induced by Mangrove (Avicennia marina) Extract in Human Breast Cancer Cells

Esau

Sagar

Bajic

et al. 2015

EJMP

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“…Mangroves have also served as a reservoir of novel species and bioactive secondary metabolites against pathogens such as MRSA (methicillin-resistant Staphylococcus aureus) [7,8]. Mangrove extracts have also been shown to have antimicrobial activities [9]. However, with the increasing rate of pollution from anthropogenic sources, mangroves are continuously exposed to pollution.…”

Section: Introductionmentioning

confidence: 99%

Insights into Antagonistic Interactions of Multidrug Resistant Bacteria in Mangrove Sediments from the South Indian State of Kerala

et al. 2019

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Antibiotic resistance is a global issue which is magnified by interspecies horizontal gene transfer. Understanding antibiotic resistance in bacteria in a natural setting is crucial to check whether they are multidrug resistant (MDR) and possibly avoid outbreaks. In this study, we have isolated several antibiotic-resistant bacteria (ARB) (n = 128) from the mangroves in Kerala, India. ARBs were distributed based on antibiotics (p = 1.6 × 10 −5 ). The 16S rRNA gene characterization revealed dominance by Bacillaceae (45%), Planococcaceae (22.5%), and Enterobacteriaceae (17.5%). A high proportion of the isolates were MDR (75%) with maximum resistance to methicillin (70%). Four isolates affiliated to plant-growth promoters, probiotics, food, and human pathogens were resistant to all antibiotics indicating the seriousness and prevalence of MDR. A significant correlation (R = 0.66; p = 2.5 × 10 −6 ) was observed between MDR and biofilm formation. Antagonist activity was observed in 62.5% isolates. Gram-positive isolates were more susceptible to antagonism (75.86%) than gram-negative (36.36%) isolates. Antagonism interactions against gram-negative isolates were lower (9.42%) when compared to gram-positive isolates (89.85%). Such strong antagonist activity can be harnessed for inspection of novel antimicrobial mechanisms and drugs. Our study shows that MDR with strong biofilm formation is prevalent in natural habitat and if acquired by deadly pathogens may create havoc in public health.

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Isolation of antibacterials from the mangrove, Avicennia marina and their activity against multi drug resistant Staphylococcus aureus

Cited by 26 publications

References 5 publications

High-Performance Thin Layer Chromatography (Hptlc) Fingerprinting Pattern of Mangrove Avicennia Marina

High-Performance Thin Layer Chromatography (Hptlc) Fingerprinting Pattern of Mangrove Avicennia Marina

Autophagy Inhibition Enhances the Mitochondrial-Mediated Apoptosis Induced by Mangrove (Avicennia marina) Extract in Human Breast Cancer Cells

Insights into Antagonistic Interactions of Multidrug Resistant Bacteria in Mangrove Sediments from the South Indian State of Kerala

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