Isolation of biochemically active chloroplasts from chlamydomonas

Mendiola-Morgenthaler, L.; Leu, Stefan; Boschetti, Arminio

doi:10.1016/0168-9452(85)90076-7

Cited by 57 publications

(41 citation statements)

References 9 publications

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“…Photosynthetically active chloroplasts have been isolated from C. reinhardtii recently (3,8,11,19) and the rates of C02-dependent 02 evolution determined here were similar. The pressure disruption method was used instead ofthe digitonin method (8) to avoid possible detergent interference with the membrane transport processes being studied.…”

Section: Resultsmentioning

confidence: 55%

“…The cell pressate contained some intact chloroplasts (3,9) along with broken chloroplasts. The intact chloroplasts have been reported to retain photosynthetic activity (3,11,19). The cell pressate has been significantly enriched for intact chloroplasts by separation in an elutriator rotor (19).…”

Section: Resultsmentioning

confidence: 99%

“…The chloroplasts obtained appeared microscopically intact and lost their ability to fix CO2 when placed in a low osmotic buffer (19). In addition, these chloroplasts had PGA-dependent 02 evolution (10)(11)(12)(13)(14)(15)(16)(17)(18)(19)(20) ,mol -h-' *mg-'Chl) that was inhibited by high (19). Therefore it is unlikely that a large percentage of the particles are intact cells.…”

Section: Resultsmentioning

confidence: 99%

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Evidence for Inorganic Carbon Transport by Intact Chloroplasts of Chlamydomonas reinhardtii

Moroney¹,

Kitayama²,

Togasaki³

et al. 1987

Plant Physiol.

100

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Isolated intact chloroplasts from wall-less mutants of Chlamydomoias reinhardtii accumulate inorganic carbon (C1) from the medium provided the cells had been adapted to low CO2 photoautotrophic growth conditions. Chloroplasts from cultures grown on high (5%) CO2 or photoheterotrophically with acetate did not accumulate inorganic carbon. Chloroplast C, accumulation from low CO2 grown cells was light dependent and was inhibited by uncouplers and inhibitors of electron transport. In a model for C, accumulation by Chiamydomonas, it is proposed that CO2 diffuses into the cell and Ci accumulation occurs in the chloroplast.

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Section: Resultsmentioning

confidence: 55%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

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Evidence for Inorganic Carbon Transport by Intact Chloroplasts of Chlamydomonas reinhardtii

Moroney¹,

Kitayama²,

Togasaki³

et al. 1987

Plant Physiol.

100

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“…Fax: (49) (211) 311-3706. studies of eukaryotic photosynthesis. The cell wall-deficient mutant CWl5 [6] can be transformed as well and furthermore is a higly suitable source of photosynthetically active chloroplasts [7]. Therefore this Chlamydomonas strain appears an ideal organism for mutagenetic studies on CFoCF~ genes with the aim to learn more about the detailed mechanism of energy transfer, catalysis and regulation of FoF~ of photosynthetic membranes.…”

Section: Introductionmentioning

confidence: 99%

Isolation of CF₀CF₁ from Chlamydomonas reinhardtii cw15 and the N‐terminal amino acid sequences of the CF₀CF₁ subunits

et al. 1995

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“…Chloroplasts were isolated from synchronized cultures of Chlamydomonas reinhardii cw-15 (stock CC-277 from the Chlamydomonas Genetics Center, Duke University, Durham, NC, USA) as described previously [21].…”

Section: Preparation Of Chloroplastsmentioning

confidence: 99%

Import inhibition of poly(His) containing chloroplast precursor proteins by Ni²⁺ ions

Rothen¹,

Thiess²,

Schumann³

et al. 1996

FEBS Letters

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The precursor of the small subunit of ribulose-l,5-bisphosphate carboxylase (pSS) and a modified pSS containing a C-terminal hexahistidyl tail (pSS(His)6) were imported into isolated Chlamydomonas chloroplasts with comparable efficiency. In the presence of Ni 2+ ions the import of pSS(His)6 was inhibited and the precursor bound to the envelope remained protease sensitive, while import of pSS was not affected. Addition of an excess of L-histidine suppressed the inhibition demonstrating that the hexahistidyl-Ni 2+ complex was responsible for import inhibition. Inhibition could be observed between about 0.5 and l0 mM Ni ~+, depending on the total protein content in the assay. Import incompetent Ni2+-precursor complexes can be used to study early events in chloroplast protein import.

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Isolation of biochemically active chloroplasts from chlamydomonas

Cited by 57 publications

References 9 publications

Evidence for Inorganic Carbon Transport by Intact Chloroplasts of Chlamydomonas reinhardtii

Evidence for Inorganic Carbon Transport by Intact Chloroplasts of Chlamydomonas reinhardtii

Isolation of CF₀CF₁ from Chlamydomonas reinhardtii cw15 and the N‐terminal amino acid sequences of the CF₀CF₁ subunits

Import inhibition of poly(His) containing chloroplast precursor proteins by Ni²⁺ ions

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Isolation of biochemically active chloroplasts from chlamydomonas

Cited by 57 publications

References 9 publications

Evidence for Inorganic Carbon Transport by Intact Chloroplasts of Chlamydomonas reinhardtii

Evidence for Inorganic Carbon Transport by Intact Chloroplasts of Chlamydomonas reinhardtii

Isolation of CF0CF1 from Chlamydomonas reinhardtii cw15 and the N‐terminal amino acid sequences of the CF0CF1 subunits

Import inhibition of poly(His) containing chloroplast precursor proteins by Ni2+ ions

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Isolation of CF₀CF₁ from Chlamydomonas reinhardtii cw15 and the N‐terminal amino acid sequences of the CF₀CF₁ subunits

Import inhibition of poly(His) containing chloroplast precursor proteins by Ni²⁺ ions