Isolation of cDNA encoding immunoglobulin light chain from common carp (Cyprinus carpioL.)

Characterization of cDNA encoding immunoglobulin light chain of the mandarin fish (Siniperca chuatsi)

“…The sequence diversity within the V and J segments constitutes the first mechanism to create antibody variability . The VL region sequences in the present study exhibited the expected variability, mostly confined to the CDRs (although a lesser degree of variability was also found in the FRs), as reported for other teleost fish (Tomana et al, 1999;Widholm et al, 1999;Lundqvist and Pilström, 1999;dos Santos et al, 2001). In addition, the length of the CDR1 (11 aa) and CDR3 (6 or 7 aa) in the clones studied matched with those of the channel catfish Ig LF isotype (Ghaffari and Lobb, 1997), but shorter than the most common length of the CDR1 (12-17 aa) and CDR3 (8-15 aa) from the Atlantic cod (Gadus morhua) (Widholm et al, 1999), Siberian sturgeon (Acipenser baeri) (Lundqvist and Pilström, 1999), and sea bass (Dicentrarchus labrax) (dos Santos et al, 2001).…”

“…The l locus is represented by multiple VL segments upstream of several JL-CL clusters (Bauser and Blomberg, 1991;Frippiat et al, 1995). The Ig H locus Veterinary Immunology and Immunopathology 95 (2003) [81][82][83][84][85][86][87][88][89][90] Abbreviations: Ig, immunoglobulin; CL, constant domain of light chain; JL, joining domain of light chain; VL, variable domain of light chain; CDR, complementarity determining region; FR, framework regionof teleost fish has a mammalian organization (Ghaffari and Lobb, 1992;Lee et al, 1993;Hordvik et al, 1997), but the organization of the Ig L locus in teleosts has been reported to be of multicluster, in which the locus is organized in L chain gene clusters, each containing VL, JL, and CL segments Ghaffari and Lobb, 1993;Tomana et al, 1999).…”

The analysis of expressed genes in the kidney of Japanese flounder, , injected with the immunostimulant peptidoglycan

“…It has been reported that both channel catfish (Gha#ari & Lobb, 1997) and rainbow trout (Partula et al, 1996) had two isotypes of IgL chains, designated as F and G, and as IgL1 and IgL2, respectively. It has also been reported in carp (Tomana et al, 1999). The amino acid sequence comparison showed that the Japanese flounder (clone M-134) CL chain was closest to the rainbow trout IgL2 isotype.…”

Characterisation of a fourth immunoglobulin light chain isotype in the common carp

“…So far it has been revealed that common carp possesses three isotypes of IgL (classified into two types according to the criterion mentioned above), L1A and L1B, both in type L1/G, and L3 in type L3/F [21,23]. Therefore, this study is aimed to identify and characterise carp L2 isotype, one of the isotypes in previously unidentified IgL types in this species.…”