Isolation of cellotriosyl blocks from barley β-glucan with endo-1,4-β-glucanase from Trichoderma reesei

Ajithkumar, Anu; Andersson, Roger; Siika‐aho, Matti; Tenkanen, Maija; Åman, Per

doi:10.1016/j.carbpol.2005.11.033

Cited by 25 publications

(11 citation statements)

References 18 publications

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“…The MALDI-ToF spectrum of barley β-glucan showed dominance of products increasing by three sugars in size, i.e., DP4, DP7, DP10, DP13, and so forth (Fig. 2C), which correlates with the known repetitive structure of this polysaccharide, from barley with every third linkage being a (1→3)-β-D linkage (29). Apparently, (1→3)-β-D linkages affect the substrate's suitability for cleavage by NcLPMO9C.…”

Section: Resultsmentioning

confidence: 96%

Discovery of LPMO activity on hemicelluloses shows the importance of oxidative processes in plant cell wall degradation

Agger

Isaksen

Várnai

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

375

366

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The recently discovered lytic polysaccharide monooxygenases (LPMOs) are known to carry out oxidative cleavage of glycoside bonds in chitin and cellulose, thus boosting the activity of well-known hydrolytic depolymerizing enzymes. Because biomass-degrading microorganisms tend to produce a plethora of LPMOs, and considering the complexity and copolymeric nature of the plant cell wall, it has been speculated that some LPMOs may act on other substrates, in particular the hemicelluloses that tether to cellulose microfibrils. We demonstrate that an LPMO from Neurospora crassa, NcLPMO9C, indeed degrades various hemicelluloses, in particular xyloglucan. This activity was discovered using a glycan microarray-based screening method for detection of substrate specificities of carbohydrate-active enzymes, and further explored using defined oligomeric hemicelluloses, isolated polymeric hemicelluloses and cell walls. Products generated by NcLPMO9C were analyzed using high performance anion exchange chromatography and multidimensional mass spectrometry. We show that NcLPMO9C generates oxidized products from a variety of substrates and that its product profile differs from those of hydrolytic enzymes acting on the same substrates. The enzyme particularly acts on the glucose backbone of xyloglucan, accepting various substitutions (xylose, galactose) in almost all positions. Because the attachment of xyloglucan to cellulose hampers depolymerization of the latter, it is possible that the beneficial effect of the LPMOs that are present in current commercial cellulase mixtures in part is due to hitherto undetected LPMO activities on recalcitrant hemicellulose structures.biorefinery | metallo enzymes | GH61 | CBM33

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Section: Resultsmentioning

confidence: 96%

Discovery of LPMO activity on hemicelluloses shows the importance of oxidative processes in plant cell wall degradation

Agger

Isaksen

Várnai

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

375

366

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“…The value of polysaccharides and derivatives in food, agriculture, and medicine has been well documented [4,5]. Polysaccharides offer healthy benefits, such as lowering blood cholesterol and blood pressure, and protecting against infections and inflammation [6].…”

Section: Introductionmentioning

confidence: 99%

Antioxidative and immunoprotective effects of Pyracantha fortuneana (Maxim.) Li polysaccharides in mice

Yuan

Wang

et al. 2010

Immunology Letters

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“…Studies have shown that adhesive effects of β-glucans can be mitigated through enzymatic hydrolysis by β-glacanases [20] . Enzymes are able to hydrolyze adhesive polymers (soluble non-starch polysaccharides) and convert them into small polymers through complete hydrolysis thereby decreasing viscosity of digesta and releasing nutrients encapsulated by these viscous compounds [20] . Viscosity increases small intestine length and weight in different ways.…”

Section: Discussionmentioning

confidence: 99%

“…It has been shown that β-glucan can enhance glucose and insulin hemostasis and decrease blood cholesterol level [21] . For this reason, it can be proposed that in the current study enzyme addition results in an increase in blood cholesterol level through digesting β-glucan content.…”

Section: Discussionmentioning

confidence: 99%

Lokal Broyler Hibridi “Golpayegani-Ross”da Performans, Karkas Karakteristiği, Mikroflora, Plazma Bileşenleri ve Bağışıklık Üzerine β-glukanazın Etkisi

Tasirnafas¹,

Karimi²,

Jafarabadi³

et al. 2019

Kafkas Univ Vet Fak Derg

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The Gram negative bacterium Fibrobacter succinogenes lives anaerobically in the bovine rumen and secretes β-glucanase. Barley is a cheap food input for poultry production but it is low in nutrients due to its beta glucan content. Supplementing exogenous β-glucanase (EC 3.2.1.4) to broiler diets can decrease the viscosity of the intestinal content by hydrolyzing the β-glucan and enhancing nutrient digestibility. In the current study, β-glucanase enzymatic activity was assayed after extraction from bovine rumen fluid, and then its effect on broiler performance, carcass characteristics, duodenum microbial flora, hematological and immunological parameters was compared with a commercial enzyme. A total of 120 local broilers (Golpayegani-Ross hybrid) was allocated to 3 treatments with 4 replicates per treatment and 10 birds per replicate/pen. Over a 49-day experimental period, broilers were fed a basal diet (T1), basal diet with 10 IU of extracted β-glucanase and 20% barley (T2) and basal diet with 10 IU of commercial β-glucanase and 20% barley (T3). T2 significantly increased body weight gain and decreased feed intake over the whole experimental period. Treatments had a significant effect on hematological parameters except low density lipoprotein (LDL) concentration (P<0.01). Treatments did not affect antibody titration. The highest non-eviscerated carcass weight (P<0.05) and eviscerated carcass weight (P>0.05) were associated with the T2. The same treatment also caused a significant increase in lactobacilli and Escherichia coli in the gastrointestinal tract. Consequently, enzyme addition had a positive effect on broiler performance without any adverse effects on humoral immunity parameters.Gram negatif bakteri Fibrobacter succinogenes, sığır rumeninde anaerobik olarak yaşar ve β-glukanaz salgılar. Arpa, kanatlı hayvan üretimi için ucuz bir besin maddesidir, ancak içerdiği beta glukan nedeniyle besin değeri düşüktür. Broyler diyetlerine eksojen β-glukanazın (EC 3.2.1.4) eklenmesi, β-glukanın hidrolize edilmesi yoluyla intestinal içeriğin viskozitesini azaltarak besinlerin sindirilebilirliğini arttırabilir. Bu çalışmada, sığır rumen sıvısından yapılan ekstraksiyondan elde edilen β-glukanazın enzimatik aktivitesi incelendi ve daha sonra broyler performansı, karkas özellikleri, duodenum mikrobiyal florası, hematolojik ve immünolojik parametreler üzerindeki etkisi ticari bir enzimle karşılaştırıldı. Toplam 120 lokal ırk broyler (Golpayegani-Ross hibrid), uygulama başına 4 tekrar ve her tekrarda 10 hayvan olacak şekilde 3 gruba ayrıldı. Kırkdokuz günlük deney süresince, broylerlere bazal diyet (T1), 10 IU ekstrakte β-glukanaz ve %20 arpa içeren bazal diyet (T2) ve 10 IU ticari β-glukanaz ve %20 arpa içeren bazal diyet (T3) verildi. Tüm deney dönemi boyunca T2 diyeti vücut ağırlığını önemli ölçüde arttırdı ve yem alımını azalttı. Tedaviler, düşük yoğunluklu lipoprotein (LDL) konsantrasyonu (P<0.01) dışındaki hematolojik parametreler üzerinde önemli bir etkiye sahipti. Bununla birlikte antikor düzeyi farklı tedavilerden etki...

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Isolation of cellotriosyl blocks from barley β-glucan with endo-1,4-β-glucanase from Trichoderma reesei

Cited by 25 publications

References 18 publications

Discovery of LPMO activity on hemicelluloses shows the importance of oxidative processes in plant cell wall degradation

Discovery of LPMO activity on hemicelluloses shows the importance of oxidative processes in plant cell wall degradation

Antioxidative and immunoprotective effects of Pyracantha fortuneana (Maxim.) Li polysaccharides in mice

Lokal Broyler Hibridi “Golpayegani-Ross”da Performans, Karkas Karakteristiği, Mikroflora, Plazma Bileşenleri ve Bağışıklık Üzerine β-glukanazın Etkisi

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