Isolation of Chlamydia trachomatis from Patients with Urethritis

Uncommon occurrence of fluoroquinolone resistance-associated alterations in GyrA and ParC in clinical strains of Chlamydia trachomatis

“…10,11 The infected cells were incubated at 37°C in 5% CO 2 for 48 h. The culture medium was replaced with SPG medium, and the infected cells were detached with a rubber policeman. Cells were disrupted by sonication to liberate intracellular C. trachomatis.…”

“…Urethral swab specimens were collected in 0.5 ml of sucrose phosphate glutamate (SPG) medium and stored at Ϫ70°C. 10 First-pass urine specimens were tested with a commercially available PCR-based assay (Amplicor CT PCR; Roche Molecular Systems, Branchburg, NJ, USA) to detect C. trachomatis.…”