Isolation of expressed sequence tags of skeletal muscle of neonatal healthy and splay leg piglets and mapping by somatic cell hybrid analysis

Maak, Steffen; Jaesert, S.; Neumann, Karsten; Yerle, Martine M.; Lengerken, G. von

doi:10.1046/j.1365-2052.2001.00778.x

Cited by 23 publications

(21 citation statements)

References 12 publications

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“…PDK4 was located at the 7q21.3-q22.1 region in human [6] and 9q12 ± 1/3q21 in swine [21,22]. Alignment between pig chromosome 9 on RH map and human maps on USDA-MARC Pig Database [23], we found some QTLs associated with meat quality and production traits in this region such as body weight at 10 weeks of age (87 cM) [24], average backfat thickness (79.3-90 cM) [25], total saturated fatty acids (61.5-83.3 cM) [26], backfat at tenth rib (96-79 cM) [27].…”

Section: Discussionmentioning

confidence: 97%

Characterization of the porcine differentially expressed PDK4 gene and association with meat quality

et al. 2008

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To investigate the differential expression of genes in the skeletal muscle between Yorkshire and Chinese indigenous breed Meishan pigs, suppression subtractive hybridization was carried out and many genes were proved to be expressed significantly different in the two breeds. One gene highly expressed in Meishan but lowly expressed in Yorkshire specific library, shared strong homology with human pyruvate dehydrogenase kinase 4 (PDK4). Using semi-quantity and quantity PCR, We confirmed its differential expression between the two breeds. Temporal and spatial expression analysis indicated that porcine PDK4 gene is highly expressed in skeletal muscle and the highest in neonatal pigs. Complete cDNA cloning and sequence analysis revealed that porcine PDK4 gene contains an open reading frame of 1,221 bp. The deduced amino acid sequence showed conservation in evolution. A G/A mutation in intron 9 was identified and association analysis showed that it was significantly associated with intramuscular fat, muscle water content.

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Section: Discussionmentioning

confidence: 97%

Characterization of the porcine differentially expressed PDK4 gene and association with meat quality

et al. 2008

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“…Maternal nutrition is undoubtedly a key factor in foetal growth and its subsequent survival which manifests its effects through a complex signalling network that includes IGFs (Hornick et al, 2000;Maak et al, 2001;Wu et al, 2006). However, in the context of foetal fibre number, the precise role of maternal nutrition is not entirely clear.…”

Section: Fibre Number: Mediators Of Muscle Hyperplasiamentioning

confidence: 99%

Key signalling factors and pathways in the molecular determination of skeletal muscle phenotype

Chang

2007

Animal

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The molecular basis and control of the biochemical and biophysical properties of skeletal muscle, regarded as muscle phenotype, are examined in terms of fibre number, fibre size and fibre types. A host of external factors or stimuli, such as ligand binding and contractile activity, are transduced in muscle into signalling pathways that lead to protein modifications and changes in gene expression which ultimately result in the establishment of the specified phenotype. In skeletal muscle, the key signalling cascades include the Ras-extracellular signal regulated kinase-mitogen activated protein kinase (Erk-MAPK), the phosphatidylinositol 3 0 -kinase (PI3K)-Akt1, p38 MAPK, and calcineurin pathways. The molecular effects of external factors on these pathways revealed complex interactions and functional overlap. A major challenge in the manipulation of muscle of farm animals lies in the identification of regulatory and target genes that could effect defined and desirable changes in muscle quality and quantity. To this end, recent advances in functional genomics that involve the use of micro-array technology and proteomics are increasingly breaking new ground in furthering our understanding of the molecular determinants of muscle phenotype.

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“…The pCDKN3 cDNA was synthesized as described elsewhere [12]. For the amplification of the coding region of the porcine CDKN3 gene, the following primer sets were used: CdknF: 5 GTG AGA CTG CCA GCC ATG AAG3 ; CdknR: 5 TTG ATG ATA GAT GTG CAG CTA ATT T3 ; KAP3F: 5 ATC GAC AGC CTG AGA GAC C3 ; KAP3R: 5 TTG CCA ATA AAA GCT TTA GGA A3 .…”

Section: Sequence Analysismentioning

confidence: 99%

“…Several biochemical and histo-morphological investigations indicate an immaturity of the skeletal muscle in the affected piglets at birth [1,2,5,14]. We have recently reported the isolation of skeletal muscle EST differentially expressed in M. biceps femoris of healthy and affected piglets [9][10][11][12]. Among the fragments strongly displayed in the splay leg muscle, we identified the porcine CDKN3 (cyclin-dependent kinase inhibitor 3) gene [13].…”

Section: Introductionmentioning

confidence: 99%

Characterization of the porcine CDKN3 gene as a potential candidate for congenital splay leg in piglets

et al. 2003

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-Congenital splay leg is a hereditary disease observed in newborn piglets. The etiology and pathogenetic mechanism of the disorder are still unknown. The gene for cyclindependent protein kinase inhibitor 3 (CDKN3) was identified as a potential candidate gene in a differential display experiment. We analyzed the gene on sequence variations and compared its expression in M. biceps femoris between healthy and affected piglets. Comparative sequencing of the coding region of three healthy and four splay leg piglets revealed twelve single nucleotide polymorphisms (SNP) resulting in six amino acid exchanges in the deduced sequences. However, all polymorphisms were observed in healthy as well as in splay leg piglets thus excluding structural differences of the gene as a cause of the disease. Besides full length transcripts, we found a variety of aberrantly transcribed cDNA in clones derived from M. biceps femoris of healthy as well as of splay leg piglets. All alternative transcripts coexist with normal cDNA. Expression analysis revealed a trend towards higher values in M. biceps femoris of splay leg piglets supporting the results obtained from a differential display.CDKN3 gene / expression analysis / congenital splay leg / piglet

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Isolation of expressed sequence tags of skeletal muscle of neonatal healthy and splay leg piglets and mapping by somatic cell hybrid analysis

Cited by 23 publications

References 12 publications

Characterization of the porcine differentially expressed PDK4 gene and association with meat quality

Characterization of the porcine differentially expressed PDK4 gene and association with meat quality

Key signalling factors and pathways in the molecular determination of skeletal muscle phenotype

Characterization of the porcine CDKN3 gene as a potential candidate for congenital splay leg in piglets

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