Isolation of Flavonoid Compound and Antioxidant Activity of Salix tetrasperma Roxb. Leaves

Januarti, Ria; Santoni, Adlis; Efdi, Mai

doi:10.24845/ijfac.v4.i2.42

Cited by 9 publications

(7 citation statements)

References 10 publications

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“…The decrease in the H/L ratio in this study was expected to be due to the activity of the phytochemical content of the Salix plant leaf extract. In Salix plants, there are bioactive compounds that act as antioxidants, including derivatives of phenolic compounds and flavonoids [ 28 , 30 , 31 , 38 – 40 ]. Various types of flavonoids and their derivatives are beneficial in birds experiencing chronic heat stress and become less effective when given to birds that are not exposed to heat [ 41 ].…”

Section: Discussionmentioning

confidence: 99%

“…The administration of Salix extract can stimulate the expression of antioxidant enzymes and prevent the formation of oxidative stress [ 28 , 29 ]. Phytochemical test results from various parts of the Salix tetrasperma plant detected the content of various secondary bioactive compounds such as catechol, salicin and their derivatives, flavonoids, phenolics, terpenoids, saponins, and steroids [ 28 , 30 , 31 ]. It is suspected that the administration of Salix extract can affect the formation of AQP2 and HSP70 compounds.…”

Section: Introductionmentioning

confidence: 99%

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Immunohistochemical Expression of AQP2 and HSP70 in Broiler Kidney Tissue Treated with Salix tetrasperma Roxb. Extract under Heat Exposure

Sugito

Etriwati

Akmal

et al. 2021

The Scientific World Journal

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The administration of plant extracts to broilers may be a way to mitigate the effects of heat stress. The importance of AQP2 and HSP70 compounds in maintaining the homeostasis of the chicken body when it is subjected to heat stress is well established. This study aims to determine the effect of giving the ethanolic extract of the leaves of Salix tetrasperma Roxb. on the immunohistochemical expression of AQP2 and HSP70 in exposed and unexposed broiler kidney tissue. This study used 36 samples of 28-day-old chicken kidneys. Chickens were kept in individual cages, provided with feed and drinking water ad libitum. The design used was a completely randomized design with 6 treatments and 6 replications: (a) chickens were reared in conditions exposed to heat (HS + 0); (b) chickens were reared in conditions exposed to heat and given Salix extract at a dose of 50 mg/L drinking water (HS + 50); (c) chickens were reared under heat-exposed conditions and given Salix extract at a dose of 100 mg/L drinking water (HS + 100); (d) chickens were reared in conditions without exposure to heat (n-HS + 0); (e) chickens were reared in conditions without exposure to heat and given Salix extract at a dose of 50 mg/L drinking water (nHS + 50); and (f) chickens were reared in conditions exposed without exposure to heat and given 100 mg/L drinking water (nHS + 100) of Salix extract. Salix extract was given for 24 hours and was renewed every 6 hours. The results showed that giving Salix extract 100 mg/L in drinking water to chickens exposed to heat (HS + 100) reduced the value of the H/L ratio. Giving Salix extract 50–100 mg/L in drinking water caused an upregulated AQP2 expression; on the other hand, it downregulated HSP-70 expression, in chicken kidney tubules both exposed to heat stress and nonexposed to heat stress. In conclusion, exposure to heat stress in broiler chickens and giving Salix extract can increase the formation of aquaporin 2 compounds and suppress the formation of HSP70.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Immunohistochemical Expression of AQP2 and HSP70 in Broiler Kidney Tissue Treated with Salix tetrasperma Roxb. Extract under Heat Exposure

Sugito

Etriwati

Akmal

et al. 2021

The Scientific World Journal

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“…The antioxidant activity was carried out by DPPH assay [32] with modifications. A total of 1 mL of 0.02 mM DPPH solution was added to a test tube containing 3 mL of the tested sample dissolved with MeOH-Acetone (9.5:0.5) in several concentrations (0.5, 1, 2, 3, 4 mg/L).…”

Section: Dpph Radical Scavenging Activity Testmentioning

confidence: 99%

Antioxidant Activity-Guided Isolation of Phenolic Compounds from Leaves of Vitex pinnata (Lamiaceae)

Faisal

Efdi

Itam

et al. 2022

CSIJ

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The aim of this study is to isolate phenolic compounds from the ethyl acetate fraction of the leaves of V. pinnata Linn, which have antioxidant properties, guided by a TLC-Bioautography (Thin-layer chromatography) with a reagent of 2,2-diphenyl-1-picrylhydrazyl radical (DPPH). The TLC-antioxidant bioautography technique has several advantages. It does not require a long time in testing, the sample used is small, and it can potentially obtain promising antioxidant-positive compounds.The isolation process led to obtaining two phenolic compounds. The UV-Vis, FTIR, and NMR spectrum analysis showed that the compounds were (1) p-hydroxybenzoate and (2) luteolin. Further antioxidant evaluation by DPPH assay showed a potential radical scavenging DPPH of luteolin (2) with an IC50 of 1.56±0.18 µg/mL.

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“…This plant has excellent sources of antioxidant agents. Reported by Januarti et al (2019), a flavonoid compound of the ethyl acetate extract of Salix tetrasperma Roxb was isolated by chromatography technique obtained 5,7-dihydroxy-3'-methoxy flavone based on NMR spectra. While antioxidant activity determined by DPPH assay obtained 5,7-dihydroxy-3'-methoxy flavone based on NMR spectra.…”

Section: Introductionmentioning

confidence: 99%

Improve the Performances of Broilers as Reduce Heat Stress by the Supplementation Jaloh (Salix tetrasperma Roxb) in Drinking Water

Zulfan

Sugito

Fitri

et al. 2022

Jurnal Sain Veteriner

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Abstrak Pencegahan dampak cekaman panas pada ayam broiler dapat dilakukan dengan pemberian ekstrak tanaman yang juga berguna dalam pengembangan obat-obat tradisional sebagai alternatif pengurangan penggunaan bahan-bahan kimiawi. Tujuan penelitian ini adalah untuk mengevaluasi performan ayam broiler yang diberikan ekstrak daun jaloh melalui air minum. Penelitian dilakukan di Laboratorium Lapangan Peternakan (LLP), Jurusan Peternakan, Fakultas Pertanian, Universitas Syiah Kuala. Sebanyak 80 ekor anak ayam broiler strain MB 202 produksi PT Japfa, Medan digunakan dalam penelitian ini. Perlakuan adalah suplementasi ekstrak daun jaloh melalui air minum pada dosis 0, 500, 1000, and 1500 ml/l air. Rancangan yang digunakan adalah Rancangan Acak Blok terdiri 4 perlakuan dan 4 blok. Tiap blok merupakan eksperimental unit yang masing-masing ditempati 5 ekor ayam. Hasil penelitian memperlihatkan walaupun secara statistik tidak berbeda nyata (P>0,05), suplementasi ekstrak daun jaloh di dalam air minum cenderung meningkatkan performan ayam broiler. Berat badan akhir, konsumsi ransum, dan konsumsi air minum meningkat sedangkan FCR menurun. Hasil penelitian disimpulkan bahwa pemberian ekstrak daun jaloh melalui air minum dapat menurunkan cekaman panas dan kematian ayam broiler dengan penggunaan terbaik pada pengenceran 1000 ml /l water air. Kata kunci: Jaloh; leaf, ekstrak, broiler, performan Abstract Prevent heat stress in broilers has been optionally created by using plants as starting points for drug development, specifically those used in traditional medicine. The objective of this study was to evaluate the performances of broiler chickens raised by offering jaloh leaf extract (JLE) through drinking water. The research has been conducted at the Field Laboratory of Animal Husbandry, Animal Husbandry Department, Syiah Kuala University. As many as 80 broiler chicks strain MB 202 produced by PT Japfa, Medan were used in this study. The treatment was supplementation JLE within drinking water at a dose of 0, 500, 1000, and 1500 ml/l of water, respectively. The research was performed into a Block Randomized Design (RBD), consisting of 4 treatments and 4 blocks. Each block was an experimental unit, each of which was placed by 5 chickens. The results of study indicated that although statistically no significant differences were detected (P>0.05) supplementation of JLE through drinking water had tendency to improve the performances of broilers. Final body weight, body weight gain, feed intake. and drinking water intake increased and FCR decreased. It was concluded that giving JLE through drinking water could minimize heat stress and reduce the mortality of broilers with the best provision of JLE at the level of 1000 ml /l water.Key words: Jaloh; leaf, extract, broiler, performances

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Isolation of Flavonoid Compound and Antioxidant Activity of Salix tetrasperma Roxb. Leaves

Cited by 9 publications

References 10 publications

Immunohistochemical Expression of AQP2 and HSP70 in Broiler Kidney Tissue Treated with Salix tetrasperma Roxb. Extract under Heat Exposure

Immunohistochemical Expression of AQP2 and HSP70 in Broiler Kidney Tissue Treated with Salix tetrasperma Roxb. Extract under Heat Exposure

Antioxidant Activity-Guided Isolation of Phenolic Compounds from Leaves of Vitex pinnata (Lamiaceae)

Improve the Performances of Broilers as Reduce Heat Stress by the Supplementation Jaloh (Salix tetrasperma Roxb) in Drinking Water

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