Isolation of Mycobacterium paratuberculosis from intestinal mucosa and mesenteric lymph nodes of goats by use of selective Dubos medium

Saxegaard, Finn

doi:10.1128/jcm.22.2.312-313.1985

Cited by 33 publications

(18 citation statements)

References 10 publications

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“…Approximately 50,000 goats produce approximately 20 million liters of milk annually, and paratuberculosis occurs endemically in goats in western Norway. The disease is controlled by vaccination, from 1967 to 2001 with a live vaccine (Saxegaard and Fodstad, 1985), and since 2001 with an inactivated vaccine (García Marín et al, 1999). In cattle, clinical paratuberculosis has occurred only sporadically, with no cases since 1979 (Djønne et al, 2001).…”

Section: Introductionmentioning

confidence: 99%

Serologic Survey for Antibodies Against Mycobacterium Avium Subsp. Paratuberculosis in Free-Ranging Cervids From Norway

Tryland

Olsen

Vikøren

et al. 2004

Journal of Wildlife Diseases

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Affinity between protein-G and immunoglobulins from red deer (Cervus elaphus), moose (Alces alces), roe deer (Capreolus capreolus), and reindeer (Rangifer tarandus tarandus) was tested in a competition binding assay. Sera from red deer, reindeer, and moose inhibited the assay less than sera from cattle (less affinity), whereas sera from roe deer showed a slightly higher affinity to protein-G than did sera from cattle. The conclusion was made that protein-G could be used instead of anti-species antibodies for these cervid species, where the aim of the screening was to look for exposure or lack of exposure to mycobacteria in the tested populations. Serologic screening of 1,373 free-ranging cervids for antibodies against Mycobacterium avium subsp. paratuberculosis was conducted. All sera were tested by a protein-G-based antigen-absorbed enzyme-linked immunosorbent assay (ELISA). Seropositive moose (10/537; 1.9%), red deer (14/371; 3.8%), roe deer (6/49; 12.2%), and semidomesticated reindeer (11/325; 3.4%) were found, whereas wild reindeer (n = 91) were seronegative. In addition, the red deer sera were tested with a commercial ELISA, by which two animals tested positive and nine were suspicious of having M. avium subsp. paratuberculosis antibodies. Tissue samples and feces from 10 moose originating from a population with a clustering of seropositive animals were investigated by histology and bacteriology with negative results. Paratuberculosis has never been diagnosed in free-ranging or farmed cervid species in Norway. Thus, further studies are indicated to prove that the present findings reflect an infection with M. avium subsp. paratuberculosis.

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Section: Introductionmentioning

confidence: 99%

Serologic Survey for Antibodies Against Mycobacterium Avium Subsp. Paratuberculosis in Free-Ranging Cervids From Norway

Tryland

Olsen

Vikøren

et al. 2004

Journal of Wildlife Diseases

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“…4 JD is a chronic inflammatory disease of ruminants caused by Mycobacterium avium subspecies paratuberculosis (MAP), a slow grower, fastidious, and intracellular pathogen. 5 Chiodini et al 6 isolated cell wall-deficient organisms, also known as spheroplast, from tissue of 6 of 28 (21.4%) patients with CD after long-term incubation culture. The spheroplast cultures then reverted to acid-fast Mycobacterium-like organism after a single in vitro passage.…”

mentioning

confidence: 99%

Evaluation of Surgical Tissue From Patients with Crohnʼs Disease for the Presence of Mycobacterium avium Subspecies paratuberculosis DNA by In Situ Hybridization and Nested Polymerase Chain Reaction

Romero

Hamdi

Valentine

et al. 2005

Inflammatory Bowel Diseases

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Crohn's disease (CD) is a chronic inflammatory bowel disease (IBD) with tissue granuloma and histopathological alteration that resembles aspects in tuberculosis, leprosy, and paratuberculosis. Mycobacterium avium subsp paratuberculosis (MAP) is the causative agent of paratuberculosis, with a suspected role in the etiology of CD. We investigated the presence of MAP DNA in 31 surgical tissue samples from 20 subjects using fluorescence in situ hybridization (FISH) with the aid of confocal scanning laser microscopy and nested polymerase chain reaction (PCR) using the IS900 sequence unique to MAP. MAP DNA was detected by PCR in tissue from 10 of 12 (83%) patients with CD: 7/12 (58%) in inflamed, 6/11 (55%) in noninflamed and in 10 (83%) of either tissue and by FISH in 8 of 12 (67%) patients with CD: 7 of 12 (58%) in inflamed, 4 of 11 (36%) in noninflamed, and in 8(67%) of either tissue. In non-IBD subjects, MAP DNA was detected in the tissue of only 1 of 6 patients (17%) by PCR and 0 of 6 patients (0%) by FISH. MAP DNA was identified by PCR in inflamed tissue from 2 of 2 patients with ulcerative colitis. The detection of MAP DNA by either technique in tissue from subjects with CD is significant compared with non-IBD subjects (P < 0.005). Identification of MAP DNA in both inflamed and noninflamed tissue by both techniques suggests that MAP infection in patients with CD may be systemic. The data add more evidence toward a possible association of MAP in the pathogenesis of CD.

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“…Sixty-five tissue fragments of about 5 to 10 g each from 26 patients with clinically active and histologically confirmed M. ulcerans infection were selected. Upon collection, the fragments were placed in 2 ml of modified Dubos medium (11) and transported to the Institute of Tropical Medicine at room temperature.…”

mentioning

confidence: 99%

Comparison of Two PCRs for Detection of Mycobacterium ulcerans

et al. 1999

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Two nested PCRs for the detection of Mycobacterium ulcerans were compared by using a collection of 65 clinical specimens. The first method amplifies the gene coding for 16S rRNA, and the second method amplifies a repetitive DNA sequence. The sensitivities of bacterioscopy, culture, 16S rRNA gene PCR, and repetitive-sequence PCR were 29, 34, 80, and 85%, respectively. Compared to the 16S rRNA gene PCR, the repetitive-sequence PCR was faster, easier to perform, and less expensive.

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Isolation of Mycobacterium paratuberculosis from intestinal mucosa and mesenteric lymph nodes of goats by use of selective Dubos medium

Cited by 33 publications

References 10 publications

Serologic Survey for Antibodies Against Mycobacterium Avium Subsp. Paratuberculosis in Free-Ranging Cervids From Norway

Serologic Survey for Antibodies Against Mycobacterium Avium Subsp. Paratuberculosis in Free-Ranging Cervids From Norway

Evaluation of Surgical Tissue From Patients with Crohnʼs Disease for the Presence of Mycobacterium avium Subspecies paratuberculosis DNA by In Situ Hybridization and Nested Polymerase Chain Reaction

Comparison of Two PCRs for Detection of Mycobacterium ulcerans

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