Isolation of Putative Progenitor Endothelial Cells for Angiogenesis

Asahara, Takayuki; Murohara, Toyoaki; Sullivan, Alison; Silver, Marcy; Zee, Rien van der; Li, Tong; Witzenbichler, Bernhard; Schatteman, Gina C.; Isner, Jeffrey M.

doi:10.1126/science.275.5302.964

Cited by 8,078 publications

(6,919 citation statements)

References 30 publications

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“…They concluded that CEC expression is related to both vascular disease and risk factors for vascular disease. CECs can be derived from the vascular wall (mature CECs), or EPCs (Asahara et al., 1997). Our results showed that CEC counts had no significant correlation with EPC counts ( p > 0.05).…”

Section: Discussionmentioning

confidence: 99%

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Circulating endothelial progenitor cells and endothelial cells in moyamoya disease

et al. 2018

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IntroductionThere is no well‐recognized biomarker for accurately predicting outcome in the presence of moyamoya disease (MMD), a progressive occlusive cerebrovascular disease of the internal carotid arteries or their branches. The aim of this study was to investigate the presence of endothelial progenitor cells (EPCs) and circulating endothelial cells (CECs) in MMD and correlate the findings with clinical features.MethodsPatients with MMD (n = 66) were compared with healthy controls (n = 81). Blood samples were obtained from an antecubital vein and analyzed using flow cytometry. EPCs were defined as CD31+ CD45dim CD34br CD133+ and CECs as CD31br CD45− CD34dim CD133−. Univariate and multivariate linear regression analyses were carried out.ResultsThe CEC counts were significantly higher in the patients than in the controls (p = 0.008). In multivariate analysis, EPC counts were independently associated with age of patients with MMD (p = 0.049) and CEC counts were independently negatively associated with concomitant disease such as hypertension, diabetes mellitus, and coronary heart disease (p = 0.034).ConclusionsThis is the first study to investigate the presence of CECs in the plasma of patients with MMD, and the amount of CECs was negatively correlated with concomitant disease in these patients.

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Section: Discussionmentioning

confidence: 99%

“…(1997). Following hypoxia or vascular injury, EPCs are recruited into the systemic circulation by secretion of various proangiogenic cytokines (Heil, Ziegelhoeffer, Mees, & Schaper, 2004; Khakoo & Finkel, 2005; Takahashi et al., 1999; Timmermans et al., 2009).…”

Section: Introductionmentioning

confidence: 99%

Circulating endothelial progenitor cells and endothelial cells in moyamoya disease

et al. 2018

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“…EPCs were isolated primarily in 1997 by Asahara et al [1], and since then has been extensively studied not only in biologic characteristics but functional implication. EPCs have been shown to express the endothelial cell surface antigens CD34, KDR and stem cell surface antigens CD133, and uptake AcLDL, so we can isolate or identify by these markers [11-15].…”

Section: Discussionmentioning

confidence: 99%

“…Since endothelial progenitor cells (EPCs) from peripheral blood (PB) were initially discovered and identified using the magnetic cells sorting method in 1997 by Asahara and his group [1], several researchers have found that EPCs existed in bone marrow, cord blood, and embryo-tissues, and that minimal number of EPCs also existed in heart, big blood vessel, skeletal muscle and fat tissue. So far, phenotypes of EPCs have remained to be determined, and exact differentiation has been still an ongoing debate topic.…”

Section: Introductionmentioning

confidence: 99%

“…So far, phenotypes of EPCs have remained to be determined, and exact differentiation has been still an ongoing debate topic. But it is widely accepted that early EPCs can be defined as CD133 + /CD34 + /KDR + cells, whereas late EPCs are positive for CD34 and VEGFR2, lose CD133 and begin to express CD31, membrane molecules typical to mature ECs [1-3]. …”

Section: Introductionmentioning

confidence: 99%

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The characteristics of bone marrow-derived endothelial progenitor cells and their effect on glioma

et al. 2012

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BackgroundEPCs were isolated primarily in 1997 by Asahara et al. and recent studies indicated that bone-marrow-derived EPCs contributed little to the endothelium of tumor vessels. Tumors of the CNS system demonstrate various features of angiogenesis.MethodsEPCs derived from rat bone marrow were isolated and cultured in M199 medium without any induced factors. EPCs were studied using immunohistochemical staining, Flow cytometry and culture under three-dimensional condition to determine EPCs’ characteristics in vitro. We also established an animal model by injecting EPCs marked with Hoechst 33342 into the back of BALB/c nude mice and performed hematoxylin-eosin (HE) and immunofluorescent staining to study EPCs’ features in vivo. To research effect of EPCs on glioma, animals bearing tumors model with C6 glioma were established. About 27 day after injection, we performed immunohistochemical staining and Immunofluorescence staining.ResultsOur results showed that EPCs derived from rat bone marrow appeared typical morphological characteristics and were positive of CD34, CD133, KDR and CD31 antigens at different time in vitro under the special M199 medium without any induced factors. The percentage of cells that expressed CD133 decreased gradually. In brief, the present study showed that EPCs derived from rat bone marrow differentiated into ECs in medium the without any induced factors and formed tubular structures in three-dimensional circumstances. Animal experiments suggested that EPCs differentiated into ECs and other else non-endothelial cells, and that EPCs contributed M199 of glioma.DiscussionThese findings provides some novel results about biological characteristics of EPCs in vivo and ex vivo, and an update on the effect of EPCs on glioma and which would be helpful for the overall understanding of EPCs and make EPCs to be implied on the clinical therapy.

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Association of Increasing Burn Severity in Mice With Delayed Mobilization of Circulating Angiogenic Cells

et al. 2010

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Angiogenesis is an important component of wound healing. Mobilization of circulating angiogenic cells (CACs) has been observed in patients with cutaneous burn wounds, but a systematic exploration of the phenomenon in an animal model has not been carried out. Using a murine model, in which burn depth can be varied precisely, and a validated culture method for quantifying circulating CACs, we found that increasing burn depth resulted in a progressive delay in the time to mobilization of circulating CACs. This delay in CAC mobilization was associated with a delay in perfusion and vascularization of the burn wound tissue. Analysis of CACs in the peripheral blood of human burn patients, using the same culture assay, confirmed results previously obtained by flow cytometry, that CAC levels peak early after the burn wound, and point to the clinical relevance of findings from the murine model.

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Isolation of Putative Progenitor Endothelial Cells for Angiogenesis

Cited by 8,078 publications

References 30 publications

Circulating endothelial progenitor cells and endothelial cells in moyamoya disease

Circulating endothelial progenitor cells and endothelial cells in moyamoya disease

The characteristics of bone marrow-derived endothelial progenitor cells and their effect on glioma

Association of Increasing Burn Severity in Mice With Delayed Mobilization of Circulating Angiogenic Cells

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