Isolation of Single Chain Variable Fragments against Six Esters of Pyrethrins by Subtractive Phage Display

Yong-yao, FU; Li, Zhengguo; Yang, Yingwu; Deng, Wei; Duan, Wei; Miao, Qing; Wang, Zhongkang; Xia, Yuxian

doi:10.1271/bbb.90043

Cited by 3 publications

(2 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The drawbacks of animal immunisation for the construction of phage display libraries include the time period required for the completion of such a process and the requirement to construct separate libraries for each antigen. However, by combining the power of immunisation with phage display, several high affinity monoclonal antibodies against "difficult" antigenic targets have been isolated from relative small antibody libraries and where traditional approaches have failed [33,98]. Generating antibodies against self or toxic antigens is limited if immunisation is to be employed.…”

Section: Phage Display Antibody Librariesmentioning

confidence: 99%

Monoclonal Antibodies and Antibody Like Fragments Derived from Immunised Phage Display Libraries

Ubah¹,

Palliyil

2017

Recombinant Antibodies for Infectious Diseases

View full text Add to dashboard Cite

Morbidity and mortality associated with infectious diseases are always on the rise, especially in poorer countries and in the aging population. The inevitable, but unpredictable emergence of new infectious diseases has become a global threat. HIV/AIDS, severe acute respiratory syndrome (SARS), and the more recent H1N1 influenza are only a few of the numerous examples of emerging infectious diseases in the modern era. However despite advances in diagnostics, therapeutics and vaccines, there is need for more specific, efficacious, cost-effective and less toxic treatment and preventive drugs. In this chapter, we discuss a powerful combinatorial technology in association with animal immunisation that is capable of generating biologic drugs with high affinity, efficacy and limited off-site toxicity, and diagnostic tools with great precision. Although time consuming, immunisation still remains the preferred route for the isolation of high-affinity antibodies and antibody-like fragments. Phage display is a molecular diversity technology that allows the presentation of large peptide and protein libraries on the surface of filamentous phage. The selection of binding fragments from phage display libraries has proven significant for routine isolation of invaluable peptides, antibodies, and antibody-like domains for diagnostic and therapeutic applications. Here we highlight the many benefits of combining immunisation with phage display in combating infectious diseases, and how our knowledge of antibody engineering has played a crucial role in fully exploiting these platforms in generating therapeutic and diagnostic biologics towards antigenic targets of infectious organisms.

show abstract

Section: Phage Display Antibody Librariesmentioning

confidence: 99%

Monoclonal Antibodies and Antibody Like Fragments Derived from Immunised Phage Display Libraries

Ubah¹,

Palliyil

2017

Recombinant Antibodies for Infectious Diseases

View full text Add to dashboard Cite

show abstract

“…high-affinity antibodies even when the library is relatively small (10 7 clones). 57,58 However, the immunization of animals is a time consuming process and each antigen requires the construction of new antibody library. Moreover, there are some limitations in the creation of human antibodies and prediction of the immune response is not always possible.…”

Section: Phage Display Technologymentioning

confidence: 99%

Phage display—A powerful technique for immunotherapy

Bazan

Całkosiński

Gamian

2012

Human Vaccines & Immunotherapeutics

197

126

View full text Add to dashboard Cite

One of the most effective molecular diversity techniques is phage display. This technology is based on a direct linkage between phage phenotype and its encapsulated genotype, which leads to presentation of molecule libraries on the phage surface. Phage display is utilized in studying protein-ligand interactions, receptor binding sites and in improving or modifying the affinity of proteins for their binding partners. Generating monoclonal antibodies and improving their affinity, cloning antibodies from unstable hybridoma cells and identifying epitopes, mimotopes and functional or accessible sites from antigens are also important advantages of this technology. Techniques originating from phage display have been applied to transfusion medicine, neurological disorders, mapping vascular addresses and tissue homing of peptides. Phages have been applicable to immunization therapies, which may lead to development of new tools used for treating autoimmune and cancer diseases. This review describes the phage display technology and presents the recent advancements in therapeutic applications of phage display.

show abstract

Novel Recombinant Antibody and Protein-based Approaches for Analysis of Food and Food Contaminants with Particular Relevance to Asia

Yamabhai

Rangnoi

Sompunga

et al. 2019

Rapid Antibody-Based Technologies in Food Analysis

View full text Add to dashboard Cite

An overview of food safety issues in Asia is presented in this chapter. An update on research and innovations related to novel recombinant antibody and protein-based approaches for analysis of food and food contaminants in Asia is reported, with China as the leading country of relevance, followed by South Korea, Japan, and Thailand. The main focus is on mycotoxins, followed by pesticide detection. Different recombinant formats, especially scFv and VHH, have been used. In addition, anti-idiotypic VHH and peptide mimotopes have been used in ELISA-based formats. For foodborne pathogens, recombinant antibodies and peptides identified using phage display technology have been used as affinity reagents for rapid detection.

show abstract

Isolation of Single Chain Variable Fragments against Six Esters of Pyrethrins by Subtractive Phage Display

Cited by 3 publications

References 34 publications

Monoclonal Antibodies and Antibody Like Fragments Derived from Immunised Phage Display Libraries

Monoclonal Antibodies and Antibody Like Fragments Derived from Immunised Phage Display Libraries

Phage display—A powerful technique for immunotherapy

Novel Recombinant Antibody and Protein-based Approaches for Analysis of Food and Food Contaminants with Particular Relevance to Asia

Contact Info

Product

Resources

About