Isolation, plant colonization potential and phenanthrene degradation performance of the endophytic bacterium Pseudomonas sp. Ph6-gfp

Abstract

This investigation provides a novel method of endophyte-aided removal of polycyclic aromatic hydrocarbons (PAHs) from plant bodies. A phenanthrene-degrading endophytic bacterium Pseudomonas sp. Ph6 was isolated from clover (Trifolium pratense L.) grown in a PAH-contaminated site. After being marked with the GFP gene, the colonization and distribution of strain Ph6-gfp was directly visualized in plant roots, stems, and leaves for the first time. After ryegrass (Lolium multiflorum Lam.) roots inoculation, strain Ph6-gfp actively and internally colonized plant roots and transferred vertically to the shoots. Ph6-gfp had a natural capacity to cope with phenanthrene in vitro and in planta. Ph6-gfp degraded 81.1% of phenanthrene (50 mg·L−1) in a culture solution within 15 days. The inoculation of plants with Ph6-gfp reduced the risks associated with plant phenanthrene contamination based on observations of decreased concentration, accumulation, and translocation factors of phenanthrene in ryegrass. Our results will have important ramifications in the assessment of the environmental risks of PAHs and in finding ways to circumvent plant PAH contamination.

Toluene Degrading Bacteria from the Rhizosphere of Solanum melongena Contaminated with Polycyclic Aromatic Hydrocarbon

“…Research of Sun et al, (2014) also confirmed that after ryegrass (Lolium multiflorum Lam.) roots inoculation, Pseudomonas sp.…”

Effect of GFP-tagging on nitrogen fixation and plant growth promotion of an endophytic diazotrophic strain ofPaenibacillus polymyxa

“…GFP is a common reporter gene used to detect and quantify endophytic colonization in plant tissues (Timmusk et al 2005;Ji et al 2014;Sun et al 2014) with the primary assumption that it is just a detection tool and the basic metabolic characteristics of the strain are not affected by D r a f t tagging. In this study, our main objective was to determine if GFP-tagging of an endophytic diazotroph, P. polymyxa P2b-2R, could affect its ability to fix N 2 and promote plant growth when inoculated onto corn.…”

“…Earlier, P. polymyxa P2b-2R was tagged with green fluorescent protein (GFP) to determine the sites of endophytic colonization in lodgepole pine (Anand and Chanway 2013a). GFP has been extensively used in detecting internal tissue colonization by endophytic diazotrophs of various genera including Paenibacillus, Bacillus, Pseudomonas, and Azospirillum (Timmusk et al 2005;Rodriguez et al 2006;Ji et al 2014;Sun et al 2014). The fundamental assumption of GFP-tagging is that it only serves as a detection tool and that the basic metabolic characteristics of the bacterial strain do not change after tagging.…”

Monitoramento da interação entre rizobactéria RZ2MS16 (<i>Burkholderia ambifaria</i>) promotora de crescimento e bioinoculantes comerciais aplicados nas culturas de soja e milho

“…Essa técnica tem sido amplamente utilizada para transformação de bactérias associadas à plantas (LACAVA et al, 2004;GAI et al, 2009;QUECINE et al, 2012;DOURADO et al, 2013;SUN et al, 2014), sendo assim adotada no presente estudo. Sendo que a SEMIA5079 apresentou um crescimento mais demorado, e a SEMIA5080 um crescimento mais rápido, atingindo a fase log às 40 h.a.i e às 8 h.a.i, respectivamente.…”