Isolation, production, purification and characterization of an organic-solvent-thermostable alkalophilic cellulase from Bacillus vallismortis RG-07

Gaur, Rajeeva; Tiwari, Soni

doi:10.1186/s12896-015-0129-9

Cited by 189 publications

(123 citation statements)

References 33 publications

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“…The key characteristic of cellulase secreted by most Bacillus sp is its activity at broad pH range (Mawadza et al 2000). Slightly similar optimum CMCase activity was found at pH 7 for B. vallismortis RG-07 (Gaur and Tiwari 2015), Bacillus sp. (Sadhu et al 2013).…”

Section: Characterization Of Cellulase Enzyme Effect Of Ph and Tempermentioning

confidence: 73%

Enhanced Cellulase Production by a Novel Thermophilic Bacillus licheniformis 2D55: Characterization and Application in Lignocellulosic Saccharification

Kazeem¹,

Shah²,

Baharuddin³

et al. 2016

BioResources

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Effects of nutritional and physicochemical factors were investigated for cellulase production by the newly isolated thermophilic strain Bacillus licheniformis 2D55 (Accession No. KT799651). The optimum cellulase production in shake flask fermentation was attained at 60 °C, pH 3.5, 180 rpm, and in a medium containing untreated sugarcane bagasse and pretreated rice husk at 7% (w/v), urea, 1 g/L, peptone, 11.0 g/L, Mg(SO4)2, 0.40 g/L, CaCl2, 0.03 g/L, Tween 80, 0.2% (w/v), and 3% inoculum. The highest carboxymethyl cellulase (CMCase), filtre paperase (FPase), and β-glucosidase produced under the optimized conditions were 29.4 U/mL, 12.9 U/mL, and 0.06 U/mL, respectively, after 18 h of fermentation. Optimization of the parameters increased the CMCase, FPase, and β-glucosidase activities by 77.4-fold, 44.5-fold, and 10-fold, respectively. The crude enzyme was highly active and stable over broad temperature (50 to 80 °C) and pH (3.5 to 10.0) ranges with optimum temperature at 65 °C and 80 ºC for CMCase and FPase, respectively. The optimum pH for CMCase and FPase was 7.5 and 6.0, respectively. Saccharification of sugar cane bagasse and rice husk by crude cellulase resulted in perspective yields of 0.348 and 0.301 g g -1 dry substrate of reducing sugars. These results suggest prospects of thermostable cellulase from B. licheniformis 2D55 in application for bio-sugar production and other industrial bioprocess applications involving high temperatures.

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Section: Characterization Of Cellulase Enzyme Effect Of Ph and Tempermentioning

confidence: 73%

Enhanced Cellulase Production by a Novel Thermophilic Bacillus licheniformis 2D55: Characterization and Application in Lignocellulosic Saccharification

Kazeem¹,

Shah²,

Baharuddin³

et al. 2016

BioResources

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“…There are very few studies that have reported on the optimum activity of cellulase by some thermophilic Bacillus species above 50 °C (Rastogi et al 2010;Annamalai et al 2013;Gaur and Tiwari 2015). Of those studies, most of them did not directly deal with enzymatic hydrolysis.…”

Section: Effect Of Hydrolysis Time On Sugar Production and Saccharifimentioning

confidence: 99%

Influence of High-Pressure Steam Pretreatment on the Structure of Rice Husk and Enzymatic Saccharification in a Two-Step System

et al. 2017

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This study aimed at developing an operational high-pressure steam pretreatment (HPSP) to effectively modify rice husk for enzymatic saccharification. The HPSP was performed at 160 to 200 °C under 0.3 to 2.8 MPa for 2 to 10 min. The efficiency of this method was based on the chemical composition, scanning electron microscopy (SEM), Fourier transform infrared (FTIR), and X-ray diffraction (XRD) analyses. Optimum pretreatment conditions (200 °C, 1.85 MPa for 7 min), enzyme concentration at 30 FPU/g and temperature at 60 °C for 48 h of continuous saccharification effectively produced sugar (21.1 g/L = 0.422 g/g dry substrate) at a saccharification degree of 53.87%. Conducting a secondstep enzymatic saccharification resulted in additional sugar production (7.9 g/L = 0.158 g/g substrate) and a 20.44% saccharification degree. In contrast, the two-step saccharification process (48 and 24 h) achieved optimal sugar yield of 0.581 g/g substrate and saccharification degree of 73.5%. Additionally, the process improved the yield of monomeric sugars of glucose (0.465 g/g), xylose (0.010 g/g), and cellobiose (0.063 g/g). Therefore, the combination of the high-pressure steam pretreatment with thermostable cellulase from Bacillus licheniformis 2D55 in a two-step enzymatic saccharification process is an economically viable method in rice husk bioprocessing for sugar production.

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“…Endoglucanases attract a lot of attention and have been widely used in various industrial application such as textile and detergent industry (Gaur and Tiwari, 2015), which maily catalyzes the hydrolysis of the cellulose by breaking the internal bonds of glucose molecules. Recently, neutral Relative pH stability of recombinant EgH31.…”

Section: Discussionmentioning

confidence: 99%

“…The biological deinking treatment can be improved with the properties of recycling fibers from waste paper, such as fiber brightness, strength properties, higher pulp cleanliness, et al (Gübitz et al, 1998;Gaur and Tiwari, 2015).…”

Section: Introductionmentioning

confidence: 99%

A Novel Neutral Endoglucanase from Streptomyces sp. H31 with Wide-pH-range Stability

Chen¹

2017

IJAB

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An endoglucanase-producing strain Streptomyces sp. H31 was isolated from mangrove soil in Shenzhen, P.R.China, and the endoglucanase gene egH31 was cloned. The open reading frame (ORF) of this gene is 762bp and it encodes 253 amino acid residues, which showed the highest similarity (84%) with the endoglucanase from Streptomyces davawensis. The endoglucanase gene egH31 was successfully expressed in E. coli. We purified the recombinant EgH31 to homogeneity by affinity chromatography. Molecular mass of the recombinant EgH31 is about 31 kDa. The maximum activity of EG-H31 was determined at pH 7.0 and 45°C and shown high stability within a wide pH range (3.0-11.0). EgH31 had good resistibility to SDS, EDTA, EGTA and most of metal ions examined. Most interestingly, EgH31 was stable in commercial laundry detergents, indicating that this enzyme is useful in textile and detergent industry.

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Isolation, production, purification and characterization of an organic-solvent-thermostable alkalophilic cellulase from Bacillus vallismortis RG-07

Cited by 189 publications

References 33 publications

Enhanced Cellulase Production by a Novel Thermophilic Bacillus licheniformis 2D55: Characterization and Application in Lignocellulosic Saccharification

Enhanced Cellulase Production by a Novel Thermophilic Bacillus licheniformis 2D55: Characterization and Application in Lignocellulosic Saccharification

Influence of High-Pressure Steam Pretreatment on the Structure of Rice Husk and Enzymatic Saccharification in a Two-Step System

A Novel Neutral Endoglucanase from Streptomyces sp. H31 with Wide-pH-range Stability

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