Isolation, propagation and preliminary characterisation of Anaplasma phagocytophilum from roe deer (Capreolus capreolus) in the tick cell line IDE8

Babesia spp. and Anaplasma phagocytophilum in questing ticks, ticks parasitizing rodents and the parasitized rodents – Analyzing the host-pathogen-vector interface in a metropolitan area

“…Detection of A. phagocytophilum was carried out in I. ricinus ticks only and in the small mammals with a real-time PCR as described [20,21]. For a subsample of the A. phagocytophilum positive samples, a nested PCR targeting a 497 bp region of the 16S rRNA gene was performed as previously described [22,23].…”

European Network for Neglected Vectors and Vector-Borne Infections COST Action Guidelines: What Is This About and What Is This For?

“…Very recently, continuous cultures of the vaccine strain of A. centrale were established for the first time in Rhipicephalus appendiculatus RAE25 and Dermacentor variabilis DVE1 cell lines . A. phagocytophilum cell cultures have also been established in several tick-derived cell lines, including I. scapularis-derived IDE8 and ISE6, Ixodes ricinus-derived IRE/CTVM19 and IRE/CTVM20 , Woldehiwet et al 2002, Silaghi et al 2011, Dyachenko et al 2013, Alberdi et al 2015, R. appendiculatusderived RAE25 and I. ricinus-derived IRE11 (Bell-Sakyi, unpublished data). ISE6 cells were also valuable for the isolation of potentially new thrombocytotropic Anaplasma closely related to A. platys (Munderloh et al 2003, Tate et al 2013.…”

“…As tick cells tolerate high cell densities and can survive for many months without subculture, there is no need to adjust cell density and inoculated cultures can be maintained for the long periods (12 weeks or more) required for adaptation of some Anaplasma spp. to in vitro growth (Silaghi et al 2011, Dyachenko et al 2013. Cultures should also be periodically evaluated by microscopy to detect any microbial growth.…”

Draft Anaplasma phagocytophilum Genome Sequences from Five Cows, Two Horses, and One Roe Deer Collected in Europe

“…In particular, there are no A. phagocytophilum genomes from horse or roe deer samples, even though EGA can have such important economic impact (2), and roe deer are suspected to play a crucial role as reservoir hosts in A. phagocytophilum epidemiology (1). Here, we present the draft genome sequences of European A. phagocytophilum from four cow and two horse samples, and two strains isolated from one cow and one roe deer which had been maintained in continuous cell cultures (3, 4). …”