Isolation, purification and characterization of a novel solvent stable lipase from Pseudomonas reinekei

Priyanka, P.; Kinsella, Gemma K.; Henehan, Gary; Ryan, Barry J.

doi:10.1016/j.pep.2018.08.007

Cited by 47 publications

(43 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This used method of screening for lipaseproducing microorganisms was reported for the first time by Kouker and Jaeger [29]. Since then, this method has been used by Castro-Ochoa and coauthors [30] and many others [31,32,33,34], this procedure has proved to be highly sensitive and reliable.…”

Section: Discussionmentioning

confidence: 99%

Arthrographis curvata and Rhodosporidium babjevae as New Potential Fungal Lipase Producers for Biotechnological Applications

Aamri

Hafidi

Scordino

et al. 2020

Braz. arch. biol. technol.

View full text Add to dashboard Cite

Fungi have always attracted a lot of attention as they are able to produce a vast repertoire of enzymes that find a broad spectrum of uses in biotechnological and industrial fields. Undoubtedly, one of the most promising biocatalysts is the lipase, which has been widely used for the biotransformation of a number of commercial products due to its high stability, high catalytic efficiency, versatility and selectivity, making it one of the most attractive and best-studied enzymes. In this study we report the isolation and molecular identification of new lipase-producing fungi from different environmental samples from Morocco. The production and activity of extracellular lipases, at different parameters, was evaluated using the Rhodamine B agar, submerged fermentation and biochemical methods. HIGHLIGHTS  This study focused on new extracellular lipase producing fungi  Identification of the selected lipase-producing fungi was carried out.  Determination of the lipase production in two different media and different pHs.  Study of temperature and pH influence on lipase activity.

show abstract

Section: Discussionmentioning

confidence: 99%

Arthrographis curvata and Rhodosporidium babjevae as New Potential Fungal Lipase Producers for Biotechnological Applications

Aamri

Hafidi

Scordino

et al. 2020

Braz. arch. biol. technol.

View full text Add to dashboard Cite

show abstract

“…Soil samples, collected from various locations on the Wicklow mountains in Ireland, were incubated in enrichment medium containing olive oil as a carbon source (Mo et al, 2016) for 72 h at 28 °C, 200 rpm. Solvent tolerant lipase producing strains were isolated as described previously (Priyanka et al, 2019). diluting the inoculum by 1:3) were selected as the inoculum for lipase production medium.…”

Section: Isolation and Identification Of The Solvent Tolerant Lipase mentioning

confidence: 99%

“…1% (w/v) of different nitrogen sources (bacteriological peptone, yeast extract, tryptone, ammonium sulfate, Urea, L-Lysine, L-Arginine, Glutamic acid, Glutamine and Asparagine respectively) were used as a substitute for 50 g/L peptone in the basal lipase production medium, as described in previous work (Priyanka et al, 2019). Following dialysis, the protein sample was transferred to an air-tight container and was stored at -80 °C for 12 h. The frozen sample was later freeze dried at -54 °C, 0.002 milli bar.…”

Section: Nitrogen Sourcementioning

confidence: 99%

“…Two lipolytic cultures isolated from soil samples (GPS location 53°00'12.4"N, 6°20'47.9"W; a woodland in a national park) were stable in cyclohexane, ethanol, n-hexane, heptane and methanol by plate overlay method (Patel, Nambiar and Madamwar, 2014). 16S rRNA sequencing of these strains identified one of the lipolytic cultures as Pseudomonas reinekei (see Priyanka et al, 2019) while the other isolate was identified as Pseudomonas brenneri. The stability of the extracellular lipase produced by Pseudomonas brenneri in the presence of n-hexane, as observed by plate overlay method, is shown in Figure 1.…”

Section: Isolation and Identification Of Solvent Tolerant Lipase Prodmentioning

confidence: 99%

“…For example, a lipase from P. cepacia has been used for the synthesis of cinnamyl propionate (Badgujar, Pai and Bhanage, 2016) while lipases from P. fluorescens and P. stutzeri have been used in the cosmetic, oral care and pharmaceutical industries (Vescovi et al, 2017;Cao et al, 2012). In this laboratory, we have previously identified lipases with potential in organic synthesis (Priyanka et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Isolation and characterization of a novel thermo-solvent-stable lipase from Pseudomonas brenneri and its application in biodiesel synthesis

Priyanka

Kinsella

Henehan

et al. 2020

Biocatalysis and Agricultural Biotechnology

Self Cite

View full text Add to dashboard Cite

Pseudomonads are one of the most studied species of bacteria as they display remarkable metabolic and physiological versatility. This enables them to colonize a wide variety of terrestrial and aquatic habitats, generating biotechnologically interesting enzymes. Here, the partial purification and characterization of a novel, extracellularly-produced, lipase from Pseudomonas brenneri is described. The partially purified lipase was active over a broad pH range (5.0-9.0) and was stable at 70 °C for 45 min. The lipase displayed significant stability, and in some cases activation, in the presence of organic solvents with log P≥2.0. Such stability characteristics indicated that this lipase could potentially be useful as a biocatalyst for biodiesel production. This was subsequently demonstrated through the facile production of Fatty Acid Methyl Esters in the presence of olive oil and methanol. Possible applications for this novel, stable lipase include the bioremediation of oil in the environment.

show abstract

Microbial lipase production: A deep insight into the recent advances of lipase production and purification techniques

Fatima

Faryad

Ataa

et al. 2020

Biotech and App Biochem

View full text Add to dashboard Cite

Importance of enzymes is ever-rising particularly microbial lipases holding great industrial worth owing to their potential to catalyze a diverse array of chemical reactions in aqueous as well as nonaqueous settings. International lipase market is anticipated to cross USD 797.7 million till 2025, rising at a 6.2% compound annual growth rate from 2017 to 2025. The recent breakthrough in the field of lipase research is the generation of new and upgraded versions of lipases via molecular strategies. For example, integration of rational enzyme design and directed enzyme evolution to attain desired properties in lipases. Normally, purification of lipase with significant purity is achieved through a multistep procedure. Such multiple step approach of lipase purification entails both conventional and novel techniques. The present review attempts to provide an overview of different aspects of lipase production including fermentation techniques, factors affecting lipase production, and purification strategies, with the aim to assist researchers to pick a suitable technique for the production and purification of lipase.

show abstract

Isolation, purification and characterization of a novel solvent stable lipase from Pseudomonas reinekei

Abstract: Abstract

Cited by 47 publications

References 37 publications

Arthrographis curvata and Rhodosporidium babjevae as New Potential Fungal Lipase Producers for Biotechnological Applications

Arthrographis curvata and Rhodosporidium babjevae as New Potential Fungal Lipase Producers for Biotechnological Applications

Isolation and characterization of a novel thermo-solvent-stable lipase from Pseudomonas brenneri and its application in biodiesel synthesis

Microbial lipase production: A deep insight into the recent advances of lipase production and purification techniques

Contact Info

Product

Resources

About