Isolation, purification, characterization, and immunomodulatory effects of polysaccharide from
            <i>Auricularia auricula</i>
            on RAW264.7 macrophages

Bao, Zhijie; Yao, Li-Qun; Zhang, Xiyue; Lin, Songyi

doi:10.1111/jfbc.13516

Cited by 27 publications

(12 citation statements)

References 49 publications

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“…Based on a thorough review of previous literature, it was found that fungal polysaccharides could normally be extracted by HWE method under the following conditions: Extraction time of 2–4 h, extraction temperature of 60–100 ℃, solid-to-liquid ratio of 1:50–1:100 g/mL ( Cai et al, 2015 , Hajji et al, 2021 , Wang et al, 2017 , Wu et al, 2010a ). Udchumpisai and Bangyeekhun, 2019 , Guo et al, 2019 , Bao et al, 2020 , and Ma et al (2016a) prepared an anti-tumor, antioxidant, immunoactive polysaccharides, and a non-toxic polysaccharides by using HWE method, respectively. Yu et al (2017) separated a polysaccharides from Flammulina velutipes with antioxidant activity by UAE under optimized extraction conditions as follows: Ultrasound power of 380 W, extraction time of 25 min, and material granularity of 400-mesh, and the yield of polysaccharide was 20.52 %.…”

Section: Extraction and Purification Methods Of Fungal Polysaccharidesmentioning

confidence: 99%

“…It was observed that GCP50-1 was an α- d -glucan with the main backbone chain of (1 → 4)-α- d -glucopyranosyl residue. Bao et al (2020) extracted Auricularia auricula polysaccharides (AAPs) by HWE and purified them to obtain three fractions (AAP-I, AAP-II, and AAP-III). AAP-I and AAP-II belonged to the glycoproteins, and only AAP-III belonged to a homogeneous pure polysaccharides.…”

Section: Structural Characteristics Of Fungal Polysaccharidesmentioning

confidence: 99%

“…Currently, fungal polysaccharides are regarded as powerful pharmacological tools, and they exhibit remarkable immunomodulatory activity by acting on immune organs or immune cells ( Zhang et al, 2017 , Zhao et al, 2019 ). A study exhibited that four extracellular polysaccharides (WPA, WPB, AP2A, and TP1A) are non-toxic to RAW264.7 cells and improved cell viability ( Bao et al, 2020 ). In addition, four extracellular polysaccharides enhanced the immunomodulatory effect via enhancing the phagocytic activity and promoting the release of TNF-α and IL-6 from RAW264.7 cells.…”

Section: Biological Activities Of Fungal Polysaccharidesmentioning

confidence: 99%

See 2 more Smart Citations

Polysaccharides from fungi: A review on their extraction, purification, structural features, and biological activities

Wang

Tan²,

Nima³

et al. 2022

Food Chemistry: X

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Section: Extraction and Purification Methods Of Fungal Polysaccharidesmentioning

confidence: 99%

Section: Structural Characteristics Of Fungal Polysaccharidesmentioning

confidence: 99%

Section: Biological Activities Of Fungal Polysaccharidesmentioning

confidence: 99%

See 1 more Smart Citation

Polysaccharides from fungi: A review on their extraction, purification, structural features, and biological activities

Wang

Tan²,

Nima³

et al. 2022

Food Chemistry: X

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“…The yield rate of AAP obtained by hot water extraction was 9.68%. Bao et al (2020) also successfully isolated A. auricula polysaccharide by hot water extraction, which showed that this method was an important method for the extraction of AAP.…”

Section: Resultsmentioning

confidence: 86%

Extraction optimisation and lipid-lowering activity of Auricularia heimuer polysaccharides

Kong¹,

Ma²,

Pan³

et al. 2021

Czech J. Food Sci.

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Assessments of molecular weight distribution and activity/efficacy of Auricularia heimuer polysaccharides (AAP) are of substantial significance for its extraction process optimisation. In the present study, single-factor orthogonal test and response surface methodology were employed to optimise extraction conditions of AAP. Furthermore, a rat hyperlipidaemia model was established to compare the lipid-lowering activity of polysaccharides obtained by three extraction methods. Conditions for enzymatic hydrolysis were optimised as pH 5.0, 1% cellulase, 2.5% substrate concentration and enzymolysis time of 1.5 h, leading to an up to 31.8% polysaccharide yield and 89.13% of polysaccharides within the molecular weight range of 5 000 Da to 10 000 Da. The results of animal experiments showed that the lipid-lowering activity of enzymolysis-extracted polysaccharides was significantly higher than that of water- and ultrasonic-extracted ones (P < 0.01). So the present study revealed that enzymatic hydrolysis-extracted polysaccharides showed the strongest hypolipidaemia activity, providing a basis for the development of A. heimuer-based functional foods and drugs.

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“…RAW264.7 cells were seeded in 24-well plates (5 × 10 5 cells/mL) and incubated for 24 h. Similarly, cells were treated as mentioned in Section 3.6.2 and then the supernatants were collected for analysis by the ELISA kits as per the manufacturer’s instructions [ 43 ].…”

Section: Methodsmentioning

confidence: 99%

Comparative Characterization and Immunomodulatory Activities of Polysaccharides Extracted from the Radix of Platycodon grandiflorum with Different Extraction Methods

et al. 2022

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Platycodon grandiflorum is an edible and medicinal plant, and polysaccharides are one of its important components. To further improve the utilization rate of P. grandiflorum, we investigated the effects of four different extraction methods, including hot water, ultrasonic-assisted, acid-assisted, and alkali-assisted extractions, on the polysaccharides, which were named PG-H, PG-U, PG-C, and PG-A. The findings indicated that the extraction method had a significant impact on the yield, characteristics, and immunoregulatory activity. We observed that the yields decreased in the following order: PG-H, PG-U, PG-C, and PG-A. Galacturonic acid, glucose, galactose, and arabinose were the most prevalent monosaccharides in the four PGs. However, their proportions varied. In addition, the difference between the content of glucose and galacturonic acid was more significant. PG-U had the highest glucose content, whereas PG-C had the lowest. Galacturonic acid content was highest in PG-A, while the lowest in PG-U. The molecular weight decreased in the order of PG-U, PG-H, PG-C, and PG-A; the particle size was in the order of PG-U, PG-A, PG-H, and PG-C. Moreover, the extraction method had a great impact on immunoregulatory activity. The ability to stimulate the immune function of macrophages was as follows: PG-A > PG-C > PG-U > PG-H. The results indicated that PGs, with lower molecular weights and higher GalA content, exhibited better immune-stimulating activity. And more important the AAE method was a good way to extract polysaccharides from Platycodon grandiflorum for use as a functional product and immunological adjuvant.

show abstract

Isolation, purification, characterization, and immunomodulatory effects of polysaccharide from Auricularia auricula on RAW264.7 macrophages

Cited by 27 publications

References 49 publications

Polysaccharides from fungi: A review on their extraction, purification, structural features, and biological activities

Polysaccharides from fungi: A review on their extraction, purification, structural features, and biological activities

Extraction optimisation and lipid-lowering activity of Auricularia heimuer polysaccharides

Comparative Characterization and Immunomodulatory Activities of Polysaccharides Extracted from the Radix of Platycodon grandiflorum with Different Extraction Methods

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