Isolation, purification, characterization and immunomodulatory effects of polysaccharides from Dictyophora rubrovalvata waste

Bao, Kaisheng; Song, Mingyang; Wang, Shanshan; Li, Tengda; Wang, Jiawen; Cheng, Xiaolei; Wang, Liuya; Wang, Siqiang; Wen, Tingchi; Zhu, Zhenyuan

doi:10.1016/j.indcrop.2023.117754

Cited by 8 publications

(2 citation statements)

References 42 publications

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“…However, some of the cells showed more obvious morphological changes, such as an increased size and the growth of pseudopods. Previous studies have shown that certain polysaccharides, such as DRP-B and ASPA80-1, can activate RAW264.7cells from a resting state to an active state, producing morphologic changes similar to those described above that increase the cells’ contact area with external substances and enhance their phagocytosis [ 16 , 42 ]. In addition, there are drugs that are often used to mimic models of inflammation that can similarly activate RAW264.7cells, such as LPS, and these drugs tend to produce more drastic morphological changes, and this overactivation induces inflammation, which can have a range of adverse effects on the body [ 43 ].…”

Section: Discussionmentioning

confidence: 88%

“…Multiple studies have demonstrated that certain mushrooms exhibit remarkable functional effects, encompassing antioxidant, immunomodulatory, and anti-tumor activities. In particular, polysaccharides in mushrooms have attracted much attention because of their high efficiency, low toxicity, and wide availability [ 13 , 14 , 15 , 16 , 17 , 18 , 19 ].…”

Section: Introductionmentioning

confidence: 99%

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Biological Characteristics, Domesticated Cultivation Protocol, Antioxidant Activity, and Protective Effects against Cellular Oxidative Stress of an Underutilized Medicinal Mushroom: Fomitopsis palustris

Liang,

Dai,

Chang

et al. 2024

JoF

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Brown-rot fungus is one of the important medicinal mushrooms, which include some species within the genus Fomitopsis. This study identified wild macrofungi collected from a broad-leaved tree in Liaoning Province as Fomitopsis palustris using both morphological and molecular methods. To elucidate the potential medicinal and economic value of F. palustris, we conducted single-factor and orthogonal tests to optimize its mycelium culture conditions. Subsequently, we completed liquid culture and domestic cultivation based on these findings. Furthermore, crude polysaccharides were extracted from the cultivated fruiting bodies of F. palustris and their antioxidant activity was evaluated using chemical methods and cell-based models. The results showed that the optimal culture conditions for F. palustris mycelium were glucose as the carbon source, yeast extract powder as the nitrogen source, pH 6.0, and a temperature of 35 °C. Moreover, temperature was found to have the most significant impact on mycelial growth. The liquid strains were fermented for 6 days and then inoculated into a cultivation substrate composed of broadleaf sawdust, resulting in mature fruiting bodies in approximately 60 days. The crude polysaccharides extracted from the cultivated fruiting bodies of F. palustris (FPPs) possess in vitro scavenging abilities against DPPH radicals and OH radicals, as well as a certain ferric-reducing antioxidant power. Additionally, FPPs effectively mitigated H2O2-induced oxidative stress in RAW264.7cells by enhancing the intracellular activity of antioxidant enzymes such as SOD and CAT, scavenging excess ROS, and reducing MDA levels. This study provides preliminarily evidence of the potential medicinal and economic value of F. palustris and offers initial data for the future development and utilization of this species.

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Section: Discussionmentioning

confidence: 88%

Section: Introductionmentioning

confidence: 99%

Biological Characteristics, Domesticated Cultivation Protocol, Antioxidant Activity, and Protective Effects against Cellular Oxidative Stress of an Underutilized Medicinal Mushroom: Fomitopsis palustris

Liang,

Dai,

Chang

et al. 2024

JoF

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Structure characterization and immunoactivity on dendritic cells of two neutral polysaccharides from Dictyophora rubrovalvata

Huang,

Yang,

Huang

et al. 2024

Nat. Prod. Bioprospect.

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Dictyophora rubrovalvata is a valuable fungus homologous to food and medicine, and its polysaccharide have been gaining increasing attention because of its plentiful activity. However, the structure and activity of its homogeneous polysaccharide have not been studied enough. In this study, two polysaccharides DRP-I and DRP-II were purified from D. rubrovalvata. Their structures were characterized by chemical composition, monosaccharide composition analysis, methylation analysis and nuclear magnetic resonance spectroscopy. The results showed that DRP-I and DRP-II were neutral heteropolysaccharides with molecular weights of 5.79 × 103 and 1.25 × 104 Da, respectively, which were composed of mannose, galactose, glucose, xylose and fucose. The main chains were → 6)-α-D-Galp-(1 → 6)-α-D-Galp-(2,1 → 6)-α-D-Manp-(2,1 → 6)-α-D-Galp-(1, and branch chains were β-D-Xylp-(1 → 3)-α-L-Fucp-(1 → 4)-α-D-Manp-(1 → and α-D-Galp-(1 → 3)-α-D-Galp-(1 → . The in vitro immunoactivity assays on dendritic cells showed that DRP-I and DRP-II could up-regulate the expression of IL-10 and IL-6 and inhibit the expression of TNF-α in a concentration-dependent manner. This research indicated that DRP-I and DRP-II possessed immunoactivity by balancing the excessive inflammation, and molecular weight is an important factor affecting immunoactivity. Graphical Abstract

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The fungi of Dictyophora genus and its by-products: Recent progress towards the development of novel food formulations

Yang,

Wang,

et al. 2024

Food Bioscience

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Isolation, purification, characterization and immunomodulatory effects of polysaccharides from Dictyophora rubrovalvata waste

Cited by 8 publications

References 42 publications

Biological Characteristics, Domesticated Cultivation Protocol, Antioxidant Activity, and Protective Effects against Cellular Oxidative Stress of an Underutilized Medicinal Mushroom: Fomitopsis palustris

Biological Characteristics, Domesticated Cultivation Protocol, Antioxidant Activity, and Protective Effects against Cellular Oxidative Stress of an Underutilized Medicinal Mushroom: Fomitopsis palustris

Structure characterization and immunoactivity on dendritic cells of two neutral polysaccharides from Dictyophora rubrovalvata

The fungi of Dictyophora genus and its by-products: Recent progress towards the development of novel food formulations

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