Isolation, Purity Assessment, and Proteomic Analysis of Endoplasmic Reticulum

Wang, Xin; Komatsu, Setsuko

doi:10.1007/978-1-0716-0528-8_9

Cited by 2 publications

(2 citation statements)

References 38 publications

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“…SER covers approximately 80 % of some glandular cells, another reason for the importance of its observation during C-L action [36]. The diameter of the SER cisternae was dramatically increased from the early 3 h, reaching its maximum at 24 h (FIG.…”

Section: )mentioning

confidence: 98%

A new crotamine-like from the rattlesnake (Crotalus durissus cumanensis) venom causing damages: Qualitative and Quantitative Cytotoxic Studies on subcellular and neuromuscular structures

et al. 2022

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A quantitative model to expose the adrenal gland sub-cellular alterations produced by crotamine-like (C-L) from rattlesnake venom during 3, 6 and 24 hours (h), and also qualitative changes on mice neuromuscular structures in vivo were observed and calculated by transmission electron microscopy. A pure crotamine-like (C-L) isoform was obtained using a cationic exchange chromatography column from the rattlesnake Crotalus durissus cumanensis venom. The C-L SDS-PAGE (15.5 %) under non-reduced conditions exhibited a molecular mass of ~3 kDa single band. The C-L in vivo qualitative experiments induced ultrastructural changes in mouse neuromuscular structures at 3, 6 and 24 h, such as reduction in the number of acetylcholine vesicles, disorganisation of the secondary synaptic clefts, enlargement of the sub-sarcolemma space and alteration of the mitochondria morphology, number and cristae. Regarding neurotoxic actions in vivo, the animals injected with C-L presented spastic paralysis of the hind limbs. The quantitative alterations studied on the capillaries, the nucleus, the mitochondria the lipid inclusions, and the smooth endoplasmic reticulum were observed from 3 to 24 h after C-L injection. As far as it is known from the literature review, there are no quantitative records of similar sub-cellular alterations caused by crotamine.

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Section: )mentioning

confidence: 98%

A new crotamine-like from the rattlesnake (Crotalus durissus cumanensis) venom causing damages: Qualitative and Quantitative Cytotoxic Studies on subcellular and neuromuscular structures

et al. 2022

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“…A refined protocol for isolating endoplasmic reticulum and assessing purity was developed, in which an isosmotic homogenization buffer consisting of HEPES, sucrose, and KCl was used for soybean root tips [ 57 ]. In wheat seedling leaves, endoplasmic reticulum protein purification was performed using HEPES and DTT.…”

Section: Crop Subcellular Proteomicsmentioning

confidence: 99%

Crop Proteomics under Abiotic Stress: From Data to Insights

Kausar

Wang

Komatsu

2022

Plants

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Food security is a major challenge in the present world due to erratic weather and climatic changes. Environmental stress negatively affects plant growth and development which leads to reduced crop yields. Technological advancements have caused remarkable improvements in crop-breeding programs. Proteins have an indispensable role in developing stress resilience and tolerance in crops. Genomic and biotechnological advancements have made the process of crop improvement more accurate and targeted. Proteomic studies provide the information required for such targeted approaches. The crosstalk among cellular components is being analyzed by subcellular proteomics. Additionally, the functional diversity of proteins is being unraveled by post-translational modifications during abiotic stress. The exploration of precise cellular responses and the networking among different cellular organelles help in the prediction of signaling pathways and protein–protein interactions. High-throughput mass-spectrometry-based protein studies are now possible due to incremental advancements in mass-spectrometry techniques, sample protocols, and bioinformatic tools as well as the increasing availability of plant genome sequence information for multiple species. In this review, the key role of proteomic analysis in identifying the abiotic-stress-responsive mechanisms in various crops was summarized. The development and availability of advanced computational tools were discussed in detail. The highly variable protein responses among different crops have provided a wide avenue for molecular-marker-assisted genetic buildup studies to develop smart, high-yielding, and stress-tolerant varieties to cope with food-security challenges.

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Isolation, Purity Assessment, and Proteomic Analysis of Endoplasmic Reticulum

Cited by 2 publications

References 38 publications

A new crotamine-like from the rattlesnake (Crotalus durissus cumanensis) venom causing damages: Qualitative and Quantitative Cytotoxic Studies on subcellular and neuromuscular structures

A new crotamine-like from the rattlesnake (Crotalus durissus cumanensis) venom causing damages: Qualitative and Quantitative Cytotoxic Studies on subcellular and neuromuscular structures

Crop Proteomics under Abiotic Stress: From Data to Insights

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