Isolation, screening and characterization of the potential microbes to enhance the conversion of food-wastes to bio-fertilizer

Ng, Su Ming; Tey, Lai-Hock; Leong, Siew Yoong; Ng, Soo Ai

doi:10.1063/1.5126583

Cited by 5 publications

(5 citation statements)

References 23 publications

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“…Bacteria with cellulolytic capacity isolated at 30 °C were cultured in 2% Starch Agar (SA) medium (3 g meat extract, 6 g NaCl, 2 g starch, 12 g agar, pH adjusted to 7, and made with 1 L deionized water). After 24 to 48 h, the plates were covered with a lugol solution (2 g of potassium iodide and 1 g of iodine in 300 mL of water) to show the clear zones of degradation due to amylase activity [ 26 ].…”

Section: Methodsmentioning

confidence: 99%

Isolation and Identification of Bacteria of Genus Bacillus from Composting Urban Solid Waste and Palm Forest in Northern Peru

Vásquez

Millones

2023

Microorganisms

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A technical challenge for composting in Peruvian cities with annual temperatures below 20 °C is that the degradation of municipal solid waste (MSW) is slow, so the identification of cold-adapted bacteria would be interesting for use as inoculants in places with these climatic conditions. This study isolated, identified, and evaluated bacterial strains with cellulolytic and amylolytic activities at low temperatures. Bacterial strains were isolated from the Chachapoyas Municipal Composting Plant and soil from the Ocol Palm Forest in northern Peru. The screening was carried out to evaluate the extracellular enzyme activity of the strains at low temperatures, grouping those with cellulolytic and cellulolytic/amylolytic activities. The DNA-barcoding using 16S rRNA and enzyme activity allowed the identification and selection of five species with enzymatic activity at 15 and 20 °C of the genus Bacillus, three with cellulolytic/amylolytic activity (B. wiedmanii, B. subtilis, and B. velezensis), and two with cellulolytic activity (B. safensis subsp. safensis, and B. subtilis). These strains showed tolerance to temperatures below optimum and could be used in further studies as inoculants for composting organic wastes at temperatures below 20 °C.

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Section: Methodsmentioning

confidence: 99%

Isolation and Identification of Bacteria of Genus Bacillus from Composting Urban Solid Waste and Palm Forest in Northern Peru

Vásquez

Millones

2023

Microorganisms

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“…The lower lipase activity suggests a relatively lower amount of lipid substrates in the MSW. Similarly, Ng et al (2019) isolated the Enterococcus sp., Staphylococcus sp., Bacillus sp., Providencia sp., and Morganella sp. activity up to 15.40 U/mL/min and 15.62 U/mL/min.…”

Section: Hydrolytic Enzyme Dynamics During Compostingmentioning

confidence: 99%

Formulation of Novel Microbial Consortia for Rapid Composting of Biodegradable Municipal Solid Waste: An Approach in the Circular Economy

Wijerathna,

Udayagee,

Idroos

et al. 2024

Environ. Nat. Resour. J.

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Urbanization and rapid industrialization have led to the escalation of municipal solid waste generation and accumulation. Composting is widely recognized as a sustainable solution for solid waste management. However, its long-term investment is considered a disadvantage. The present research study discusses the rapid biotransformation of solid waste into valorized compost. Bacteria were isolated from soil, solid waste, and leachate samples from open dump sites. From the 18 different bacterial consortia created using potential isolates, the five most promising consortia were selected based on concurrent different enzyme production. These selected consortia were incorporated into typical compost bins with Municipal Solid Waste (MSW). Daily monitoring of enzymatic activity, pH, conductivity, bulk density, moisture, and temperature, along with other composting parameters, was conducted. The study’s results demonstrated that consortium No. 5, comprising Bacillus haynesii, Bacillus amyloliquefaciens, and Bacillus safensis, exhibited significant (p<0.05) enzyme activity of cellulase, amylase, lipase and proteinase enzymes during composting compared to the control and other treatment setups. Consortium No. 5 also facilitated rapid and successful composting, as evidenced by significant alterations of composting parameters by exhibiting a shorter average composting time, reducing it from 110±10 days to 20±3 days, showcasing the potential applicability of formulated bacterial consortium as a sustainable and greener approach to the global solid waste problem. The novelty of this study lies in the isolation of local bacterial strains from open dump sites soil, MSW, and MSW leachate samples, which were then utilized in the composting organic fraction of MSW, enhancing the potential for effective waste management.

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“…Black soldier fly (BSF) merupakan salah satu organisme yang potensial yang dapat mendegradasi bahan organik dengan nutrisi minimal secara cepat. Hal ini karena pencernaan BSF merupakan habitat bakteri amilolitik (Ng et al, 2019). Keberadaan bakteri dalam usus larva tersebut membantu BSF dalam mengkonversi limbah organik menjadi lemak dan protein menjadi biomassa tubuhnya (Zheng et al, 2011).…”

Section: Pendahuluanunclassified

Aktivitas amilase bakteri amilolitik asal larva black soldier fly (Hermetia illucens)

Kresnawaty¹,

Wahyu

Sasongko

2019

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Amylase is an enzyme that has been widely used as a biocatalyst in foodand bioethanol industries. The availability of thermostable amylase will further expand the market and extend the shelf life of this enzyme. Amylase is produced by amylolytic bacteria using media with high-costnitrogen sources, such as pepton. Black soldier fly (BSF) is a potential source of amylolitic bacteria since its ability to degrade organic matters rapidly. This research aimedtoexploreamylolitic bacteria from the larvae of BSF with highest amylase activity that can be produced using low-cost media. The screening ofamylase activity was conducted by culturing the bacteria on starch containing media.Bacteria with the highest amylase activity were cultured in liquid media with twodifferentnitrogensources (urea and nitrate). Determinations of the optimum pH and temperature for this enzyme activity were carried out in the pH range 4to 7 and temperature 35to 65 ºC. Three amylase-producing isolates were obtained in this study. M1 isolate which has the highest activity was characterized based oncatalase activity and Gram staining. The results showed that the M1 isolate mightbelong togenus Proteussp. At the optimum condition (45ºC and pH 7), amylase activityin nitrate mediawas0.791U/mL, which was about 18-folds higher than that in ureamedia (0,041U/mL). Thus, amylase isolated from BSF larvae can be classified as a mesophilic enzyme and has the potential to be developed commercially at lower production costs.[Keywords:crude extract enzyme,Proteus sp.,thermostable] AbstrakAmilase merupakan salah satu enzim yangtelah digunakan secara luas sebagai biokatalis dalam industri pangan dan bioetanol.Ketersediaan amilase termostabil akan semakin memperluas pasar dan memperpanjang daya simpan enzim ini. Selama ini, produksi amilase dilakukan dengan memanfaatkan bakteri amilolitik menggunakan media dengan sumber nitrogen yang mahal, misalnya pepton. Black soldier fly (BSF) merupakan sumber bakteri amilolitik yang potential karena BSF memiliki kemampuan mendegradasi bahan organik dengan cepat. Penelitian ini bertujuan untuk mengeksplorasi bakteri amilolitik dengan kemampuan amilase tinggi yang dapat diproduksi menggunakan media yanglebihmurah.Skrining bakteri penghasil amilase dilakukan dengan menumbuhkan bakteri pada media yang mengandung pati. Bakteri dengan aktivitas amilase tertinggi dikulturkan dalam media cair dengan dua sumber nitrogenyang berbeda, yaitu urea dan nitrat. PenentuanpH dan suhu optimum aktivitas enzim ini dilakukan pada rentang pH 4sampai 7 dan suhu 35sampai 65 ºC.Tiga isolat penghasil amilase diperoleh dalam penelitian ini. Isolat M1 yang memiliki aktivitas tertinggi dikarakterisasi berdasarkan uji katalasedan uji pewarnaan Gram. Hasilnya menunjukkan bahwa isolat M1 termasukgenus Proteus sp. Pada kondisi optimum (suhu 45oC dan pH 7), aktivitas amilase pada media nitrat adalah 0,791 U/mL, lebih kurang 18 kali lebih tinggi dibanding aktivitas pada media urea (0,041 U/mL). Dengan demikian, amilase yang dihasilkan oleh bakteri asallarva BSF merupakan enzim mesofilik dan berpotensi untuk dikembangkan secara komersial dengan biaya produksi yang lebih murah.[Kata kunci: enzim ekstrak kasar, Proteussp.,termostabil]

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Isolation, screening and characterization of the potential microbes to enhance the conversion of food-wastes to bio-fertilizer

Cited by 5 publications

References 23 publications

Isolation and Identification of Bacteria of Genus Bacillus from Composting Urban Solid Waste and Palm Forest in Northern Peru

Isolation and Identification of Bacteria of Genus Bacillus from Composting Urban Solid Waste and Palm Forest in Northern Peru

Formulation of Novel Microbial Consortia for Rapid Composting of Biodegradable Municipal Solid Waste: An Approach in the Circular Economy

Aktivitas amilase bakteri amilolitik asal larva black soldier fly (Hermetia illucens)

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