Isolation, screening, and characterization of surface-active agent-producing, oil-degrading marine bacteria of Mumbai Harbor

Mohanram, Rajamani; Jagtap, Chandrakant B.; Kumar, Pradeep

doi:10.1016/j.marpolbul.2016.02.040

Cited by 40 publications

(24 citation statements)

References 45 publications

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“…Six isolates, i.e., IO-1, IO-6, IO-8, IO-19, IO-24 and IO-25, had the capacity to form a clear zone and an oil displacement area varying from 0.56 cm to 2.02 cm and from 3.53 cm 2 to 12.67 cm 2 , respectively. Such criteria have been used previously to assess the hydrocarbon-degrading activity of isolated strains (Pacwa-Płociniczak et al., 2016; Mohanram et al., 2016). The importance of the clear zone diameter and oil displacement area might be directly related to the production of secondary metabolites, such as biosurfactant (Eubeler et al., 2010; Hidayati et al., 2011).…”

Section: Resultsmentioning

confidence: 99%

Culturable hydrocarbonoclastic marine bacterial isolates from Indonesian seawater in the Lombok Strait and Indian Ocean

Syakti

Lestari

Simanora

et al. 2019

Heliyon

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A B S T R A C TPurpose: The study aims to isolate the culturable marine bacteria and to assess their potential as the bioremediation agent for petroleum hydrocarbons contamination in marine environment. Methods: Bacteria isolates were obtained by repetitive streaks to obtain purified bacteria on Zobell marine agar plates before further analysis and culture through direct visualization on agar plates. Identification were conducted using 16S rDNA sequence which are compared using NCBI BLAST and, combined with phenotypic and phylogenetic data. The potential use of the selected bacteria was tested by culturing them with two carbon sources i.e., glucose and crude oil.Result: Fifty-one culturable marine hydrocarbonoclastic bacteria were isolated from the Lombok Strait (LS-3, LS-13, LS-14, LS-15, and Indian Ocean (IO-1, IO-6, IO-8, IO-19, IO-24 and IO-25). Twelve isolates were found to degrade crude oil efficiently at a >2% concentration and to grow with crude oil as their sole carbon and energy source. These 12 strains belong to the genus Bacillus, which is well known to produce surface active agents, and the oil displacement assay indicated the production of these agents by these strains. Within the genera Bacillus, five species (Bacillus flexus, B. methylotrophicus, B. aquimaris, B. horikoshii, and B. thioparans) were represented by the 12 identified strains. Conclusion: Selected strains from the Lombok Strait and Indian Ocean were capable of degrading crude oil (2% v/ v) by 43.9-71.9% over 14 days. These results are important for marine bioremediation in Indonesia, which often faces risks of oil spill contamination and disaster.

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Section: Resultsmentioning

confidence: 99%

Culturable hydrocarbonoclastic marine bacterial isolates from Indonesian seawater in the Lombok Strait and Indian Ocean

Syakti

Lestari

Simanora

et al. 2019

Heliyon

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“…This assay can be carried out in 96-well microplates, which indicates high-throughput screening (Fig. 3 a) [ 180 , 181 ]. An adaptation of this method is the use of a hydrophobic surface of the commercial product parafilm-M ™ (Parafilm-M Test) to facilitate visualization (Fig.…”

Section: Screening Of Biosurfactant-producing Fungi: Methodologies and Characterizationmentioning

confidence: 99%

Fungal biosurfactants, from nature to biotechnological product: bioprospection, production and potential applications

Silva

Banat

Giachini

et al. 2021

Bioprocess Biosyst Eng

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Biosurfactants are in demand by the global market as natural commodities that can be added to commercial products or use in environmental applications. These biomolecules reduce the surface/interfacial tension between fluid phases and exhibit superior stability to chemical surfactants under different physico-chemical conditions. Biotechnological production of biosurfactants is still emerging. Fungi are promising producers of these molecules with unique chemical structures, such as sophorolipids, mannosylerythritol lipids, cellobiose lipids, xylolipids, polyol lipids and hydrophobins. In this review, we aimed to contextualize concepts related to fungal biosurfactant production and its application in industry and the environment. Concepts related to the thermodynamic and physico-chemical properties of biosurfactants are presented, which allows detailed analysis of their structural and application. Promising niches for isolating biosurfactant-producing fungi are presented, as well as screening methodologies are discussed. Finally, strategies related to process parameters and variables, simultaneous production, process optimization through statistical and genetic tools, downstream processing and some aspects of commercial products formulations are presented.

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“…The red pigmented colony was selected and detailed morphological, biochemical and molecular biology characterization was done. The red pigmented bacterial isolate was labelled as strain NMRL65 and stored in glycerol stock at -80°C for further characterization [6].…”

Section: Isolation Of Bacteria From Pinkish Red Discoloured Spoiled Cmentioning

confidence: 99%

Isolation, Identification and Molecular Characterization of Serratia Marcescens Strain Nmrl65 an Opportunistic Human Pathogen From the Spoiled Coconut

2020

IJEHS

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Microbes are the common cause of food spoilage and are widely distributed in the environment. Fruits and vegetables provide ideal nutrient conditions for the survival and growth of many types of bacteria. Bacteria responsible for food spoilage produce extracellular hydrolytic enzymes which bring about the deterioration of the food products. Coconut flesh is highly nutritious and rich in saturated fat, sugar and potassium that have wide range of health benefits. Fresh coconut flesh is often spoiled by red pigment producing bacterium Serratia. The present study was therefore undertaken and a red pigment producing opportunistic human pathogen Serratia marcescens strain NMRL 65 from spoiled coconut was isolated, identified and characterized for specific genes. Also its ability to produce multiple hydrolytic extracellular enzymes like chitinase, lipase and protease was investigated and reported.

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Isolation, screening, and characterization of surface-active agent-producing, oil-degrading marine bacteria of Mumbai Harbor

Cited by 40 publications

References 45 publications

Culturable hydrocarbonoclastic marine bacterial isolates from Indonesian seawater in the Lombok Strait and Indian Ocean

Culturable hydrocarbonoclastic marine bacterial isolates from Indonesian seawater in the Lombok Strait and Indian Ocean

Fungal biosurfactants, from nature to biotechnological product: bioprospection, production and potential applications

Isolation, Identification and Molecular Characterization of Serratia Marcescens Strain Nmrl65 an Opportunistic Human Pathogen From the Spoiled Coconut

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