Isolation, Screening and Characterization of Cellulase Producing Bacterial Isolates from Municipal Solid Wastes and Rice Straw Wastes

Khatiwada, Prabesh; Ahmed, Jahed; Sohag, Md. Mehadi Hasan; Islam, Md. Kamrul; Azad, Abul Kalam

doi:10.4172/2155-9821.1000280

Cited by 13 publications

(6 citation statements)

References 26 publications

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“…These findings were in agreement with the previously reported by (Abdullah et al, 2016) and (Khatiwada et al, 2016).…”

Section: Production Extraction and Purification Of Cellulase Of The Isolate Eg7supporting

confidence: 94%

Isolation and identification of cellulase producing Bacillus subtilis EG7 and its potential in composting of agricultural wastes

2017

Int. J. Biosci.

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In the current study, some bacterial isolates capable of utilizing Rice straw as an agricultural waste were obtained. Among them, the cellulolytic activity of the isolate EG7, detected both qualitatively and quantitatively, was promising. This isolate degraded the different cellulosic substrates used; Carboxymethylcellulose, Avicel, Cotton seed linters and Rice straw. The isolate was identified by classical bacteriological examination, metabolic fingerprinting using Biolog MicroPlate and phylogenetic analysis of 16S ribosomal Ribo-Nucleic Acid (16SrRNA) gene nucleotide sequence as Bacillus subtilis, GenBank accession number (KX817281). Cellulase enzyme of the isolate EG7 was produced under the determined optimum conditions. Among these conditions, static incubation for three days at initial pH 7.00 and incubation temperature 40 o C with cellulose concentration 10 % (w/v) were favorable. Under these conditions, the enzyme activity recorded 45.00 UmL -1 . Purification of the produced enzyme was performed and the results revealed that the enzyme activity recorded 64.50UmL -1 . Molecular weight determination revealed the presence of two distinct bands of about 43 and 37 KDa. The use of the isolate EG7 in composting of Rice straw and Banana peels waste resulted in enhancing the process and reducing time of composting into five to seven days. Our results indicated the potential of using the isolate EG7 in enhancing composting of agricultural wastes, besides, the use of its cellulase in the different industrial applications.

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“…These findings were in agreement with the previously reported by (Abdullah et al, 2016) and (Khatiwada et al, 2016).…”

Section: Production Extraction and Purification Of Cellulase Of The Isolate Eg7supporting

confidence: 94%

Isolation and identification of cellulase producing Bacillus subtilis EG7 and its potential in composting of agricultural wastes

2017

Int. J. Biosci.

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“…The obtained P. taiwanensis U3 isolate from peat soil was supported by research by Situmorang et al (2015) and Gusmawartati et al (2017), who successfully isolated Pseudomonas from the soil habitats. Besides Sethi et al (2013), Khatiwada et al (2016), Maharsiwi et al (2020) reported that Pseudomonas is one of the cellulolytic bacteria which showed cellulose hydrolysis activity. This study is the first report that unravels P. taiwanensis U3 ability to degrade cellulose in an acidic environment.…”

Section: Cellulolytic Bacteriamentioning

confidence: 99%

Cellulose degrading bacteria isolated from Palangkaraya, Central Kalimantan, Indonesia as peat fiber decomposer to accelerate peat soil compression

Zulaika

SOLIKHAH²,

UTOMO³

et al. 2022

Biodiversitas

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Abstract. Zulaika E, Solikhah F, Utomo MAP, Endah N, Assavalapsakul W. 2022. Cellulose degrading bacteria isolated from Palangkaraya, Central Kalimantan, Indonesia as peat fiber decomposer to accelerate peat soil compression. Biodiversitas 23: 1648-1654. Peat soil was established from incomplete weathered plants. It delays the process of soil compression. The main components of peat soil are fibers which made of cellulose and lignin that can be enzymatically degraded by cellulolignolytic bacteria. The objective of this study was to isolate indigenous lignocellulolytic bacteria from peat soil that perform peat fiber degradation to accelerate soil compression process. A bacteria screening test was carried out on carboxymethyl cellulose (CMC) media and bacteria identification using 16S rRNA gene markers. Peat fiber decomposition test based on biomass loss, decomposition results were visualized using scanning electron microscopy (SEM) and analyzed using Fourier Transform Infrared (FTIR). The results of screening and identification obtained 5 bacterial species, namely Pseudomonas taiwanensis U3 and Bacillus cereus D1, D3, U2, U4. After six weeks of incubation, P. taiwanensis U3 showed the highest cellulase activity than other isolates with 90% decomposition ability of peat fibers. The results of the decomposition of peat fibers with the addition of isolates visualized smaller pore sizes than control. Stretching occurs in the aromatic group of the C-O ester bond, the CºC structure of the alkyne group, and the O-H of the alcohol. All obtained bacteria isolates were still viable after six weeks of incubation with 104 CFU g-1 dry weight of fiber. The present investigation deals with isolating indigenous lignocellulolytic bacteria from peat soil that perform peat fiber degradation to accelerate the soil compression process. Our findings are also the first publication that reported P. taiwanensis cellulolytic potential.

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“…Estos resultados coinciden con los publicados para las celulasas producidas por B. licheniformis MTCC429, a partir de residuos agroindustriales (Bala Kumaran et al 2015), por las obtenidas por Pseudomonas sp. y Serratia sp., aisladas de residuos de salvado de trigo (Prabesh et al 2016) y para la producida a partir de Acinetobacter junii GAC 16.2 (Banerjee et al 2020).…”

Section: Resultados Y Discusiónunclassified

Obtención de celulasas bacterianas usando residuos orgánicos generados en plazas de mercado

Sánchez-Castelblanco,

Heredia-Martín

2024

Rev. U.D.C.A Act. & Div. Cient.

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Los residuos agrícolas son una fuente de celulosa que puede ser aprovechada para producir enzimas hidrolíticas, como las celulasas, mediante acción microbiana. Estas celulasas son utilizadas en procesos extractivos de biomoléculas, en la producción de biogás, en la industria textil, detergente, alimentaria y del papel. El propósito del estudio es la obtención de celulasas bacterianas utilizando residuos orgánicos, generados en plazas de mercado. Se realizaron dos medios de cultivo (A1 y A2), a partir de ameros de mazorca y cáscaras de leguminosas, ajustando el pH del medio A1 a 7,0 y el de A2 a 5,0. Los medios fueron fermentados por 75 horas, mediante la cepa bacteriana C6M2, aislada de residuos de plazas de mercado, monitoreando la actividad enzimática, la concentración de azúcares reductores y la celulosa residual. La máxima actividad celulolítica se logró a las 56 horas de fermentación en A1 y a las 32 horas, en A2. El extracto enzimático se precipitó, dializó y ultrafiltró, obteniendo una actividad final de 9,07 ± 0,48 U/mL. Los ameros y las cáscaras de leguminosa se pueden aprovechar como sustratos en la producción de celulasas, con posibles aplicaciones en procesos donde requieran bajos grados de pureza.

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Isolation, Screening and Characterization of Cellulase Producing Bacterial Isolates from Municipal Solid Wastes and Rice Straw Wastes

Cited by 13 publications

References 26 publications

Isolation and identification of cellulase producing Bacillus subtilis EG7 and its potential in composting of agricultural wastes

Isolation and identification of cellulase producing Bacillus subtilis EG7 and its potential in composting of agricultural wastes

Cellulose degrading bacteria isolated from Palangkaraya, Central Kalimantan, Indonesia as peat fiber decomposer to accelerate peat soil compression

Obtención de celulasas bacterianas usando residuos orgánicos generados en plazas de mercado

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