Isolation, Sequencing, and Analysis of a 14-3-3 Brain Protein Homolog from Pea (Pisum sativum L.)

Stanković, Bratislav; Garic-Stankovic, A.; Smith, C. Michael; Davies, E.

doi:10.1104/pp.107.4.1481

Cited by 6 publications

(4 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Little work is done on this gene in terms of its role in the defense response. Homologs of this 14-3-3 gene are cloned from Cucurbita pepo (Markiewicz et al 1994), A. thaliana (Rooney and Ferl 1995), Pisum sativum (pea) (Stankovic et al 1995), Solanum lycopersicum (tomato) (Roberts and Bowles 1999) and other plants. The cloning of this protein in tomato and subsequent work demonstrate that the gene responds rapidly, within 15 min after mechanical damage (O'Donnell et al 1998).…”

Section: -3-3 Protein Expressionmentioning

confidence: 99%

A gene expression analysis of syncytia laser microdissected from the roots of the Glycine max (soybean) genotype PI 548402 (Peking) undergoing a resistant reaction after infection by Heterodera glycines (soybean cyst nematode)

et al. 2009

View full text Add to dashboard Cite

The syncytium is a nurse cell formed within the roots of Glycine max by the plant parasitic nematode Heterodera glycines. Its development and maintenance are essential for nematode survival. The syncytium appears to undergo two developmental phases during its maturation into a functional nurse cell. The first phase is a parasitism phase where the nematode establishes the molecular circuitry that during the second phase ensures a compatible interaction with the plant cell. The cytological features of syncytia undergoing susceptible or resistant reactions appear the same during the parasitism phase. Depending on the outcome of any defense response, the second phase is a period of syncytium maintenance (susceptible reaction) or failure (resistant reaction). In the analyses presented here, the localized gene expression occurring at the syncytium during the resistant reaction was studied. This was accomplished by isolating syncytial cells from Glycine max genotype Peking (PI 548402) by laser capture microdissection. Microarray analyses using the Affymetrix soybean GeneChip directly compared Peking syncytia undergoing a resistant reaction to those undergoing a susceptible reaction during the parasitism phase of the resistant reaction. Those analyses revealed lipoxygenase-9 and lipoxygenase-4 as the most highly induced genes in the resistant reaction. The analysis also identified induced levels of components of the phenylpropanoid pathway. These genes included phenylalanine ammonia lyase, chalcone isomerase, isoflavone reductase, cinnamoyl-CoA reductase and caffeic acid O-methyltransferase. The presence of induced levels of these genes implies the importance of jasmonic acid and phenylpropanoid signaling pathways locally at the site of the syncytium during the resistance phase of the resistant reaction. The analysis also identified highly induced levels of four S-adenosylmethionine synthetase genes, the EARLY-RESPONSIVE TO DEHYDRATION 2 gene and the 14-3-3 gene known as GENERAL REGULATORY FACTOR 2. Subsequent analyses studied microdissected syncytial cells at 3, 6 and 9 days post infection (dpi) during the course of the resistant reaction, resulting in the identification of signature gene expression profiles at each time point in a single G. max genotype, Peking.

show abstract

Section: -3-3 Protein Expressionmentioning

confidence: 99%

A gene expression analysis of syncytia laser microdissected from the roots of the Glycine max (soybean) genotype PI 548402 (Peking) undergoing a resistant reaction after infection by Heterodera glycines (soybean cyst nematode)

et al. 2009

View full text Add to dashboard Cite

show abstract

“…The largest class of proteins was comprised of stress and defense-related enzymes and proteins including histone H4 (Patat et al, 2004), chitinase, heat shock and dehydration responsive proteins, and reactive oxygenrelated enzymes such as lipoxygenase, peroxidase, and superoxide dismutase (Kawano, 2003). Also present were such cytoplasmic markers as 14-3-3 proteins (Stankovic et al, 1995), ribosomal proteins, and a cytochrome P450. Only 20 proteins, including the cytochrome P450, matched proteins with predicted signal peptides (Table II).…”

Section: Identification Of Proteins Of the Root Cap Secretomementioning

confidence: 99%

Extracellular Proteins in Pea Root Tip and Border Cell Exudates

et al. 2006

View full text Add to dashboard Cite

Newly generated plant tissue is inherently sensitive to infection. Yet, when pea (Pisum sativum) roots are inoculated with the pea pathogen, Nectria haematococca, most newly generated root tips remain uninfected even though most roots develop lesions just behind the tip in the region of elongation. The resistance mechanism is unknown but is correlated spatially with the presence of border cells on the cap periphery. Previously, an array of .100 extracellular proteins was found to be released while border cell separation proceeds. Here we report that protein secretion from pea root caps is induced in correlation with border cell separation. When this root cap secretome was proteolytically degraded during inoculation of pea roots with N. haematococca, the percentage of infected root tips increased from 4% 6 3% to 100%. In control experiments, protease treatment of conidia or roots had no effect on growth and development of the fungus or the plant. A complex of .100 extracellular proteins was confirmed, by multidimensional protein identification technology, to comprise the root cap secretome. In addition to defense-related and signaling enzymes known to be present in the plant apoplast were ribosomal proteins, 14-3-3 proteins, and others typically associated with intracellular localization but recently shown to be extracellular components of microbial biofilms. We conclude that the root cap, long known to release a high molecular weight polysaccharide mucilage and thousands of living cells into the incipient rhizosphere, also secretes a complex mixture of proteins that appear to function in protection of the root tip from infection.

show abstract

“…Studies of 14‐3‐3s in plants comprised the cloning of several isoforms in spinach and Oenothera [11], pea [12], maize [13], Arabidopsis [14, 15], and other plant species. Phylogenetic analyses indicated an early and clear separation of the plant and animal lineages [3, 16].…”

Section: Introductionmentioning

confidence: 99%

Over‐expression of plant 14‐3‐3 proteins in tobacco: enhancement of the plasmalemma K⁺ conductance of mesophyll cells¹²

et al. 1997

View full text Add to dashboard Cite

Two cDNA clones encoding 14-3-3 homologous proteins were isolated from Vicia faba. Deduced amino acid sequences share different degrees of homology with other plant 14-3-3 proteins. Both clones, under the control of the CaMV 35S promoter, were transformed into tobacco plants. Immunoblotting showed three different forms of ca. 31, 34, and 37 kDa, indicating a covalent modification of the expressed 14-3-3 proteins. These forms were mainly present in the microsomal fraction. Patch-clamp studies of mesophyll protoplasts of the transformants revealed a strongly enhanced K + conductance compared to the wild type. This indicates the involvement of 14-3-3 proteins in ion channel regulation, presumably by modulating kinase activities or binding the channel.

show abstract

Isolation, Sequencing, and Analysis of a 14-3-3 Brain Protein Homolog from Pea (Pisum sativum L.)

Cited by 6 publications

References 6 publications

A gene expression analysis of syncytia laser microdissected from the roots of the Glycine max (soybean) genotype PI 548402 (Peking) undergoing a resistant reaction after infection by Heterodera glycines (soybean cyst nematode)

A gene expression analysis of syncytia laser microdissected from the roots of the Glycine max (soybean) genotype PI 548402 (Peking) undergoing a resistant reaction after infection by Heterodera glycines (soybean cyst nematode)

Extracellular Proteins in Pea Root Tip and Border Cell Exudates

Over‐expression of plant 14‐3‐3 proteins in tobacco: enhancement of the plasmalemma K⁺ conductance of mesophyll cells¹²

Contact Info

Product

Resources

About

Isolation, Sequencing, and Analysis of a 14-3-3 Brain Protein Homolog from Pea (Pisum sativum L.)

Cited by 6 publications

References 6 publications

A gene expression analysis of syncytia laser microdissected from the roots of the Glycine max (soybean) genotype PI 548402 (Peking) undergoing a resistant reaction after infection by Heterodera glycines (soybean cyst nematode)

A gene expression analysis of syncytia laser microdissected from the roots of the Glycine max (soybean) genotype PI 548402 (Peking) undergoing a resistant reaction after infection by Heterodera glycines (soybean cyst nematode)

Extracellular Proteins in Pea Root Tip and Border Cell Exudates

Over‐expression of plant 14‐3‐3 proteins in tobacco: enhancement of the plasmalemma K+ conductance of mesophyll cells12

Contact Info

Product

Resources

About

Over‐expression of plant 14‐3‐3 proteins in tobacco: enhancement of the plasmalemma K⁺ conductance of mesophyll cells¹²