Isolation, Simultaneous Quantification of Taxifolin and Taxifolin-3-O-rhamnoside and Validation by RPHPLC

Abstract

Background: Taxifolin (TA) is a flavonoid that has antioxidant, hepatoprotective, antialzheimer, anti-hyperglycemic, cardiovascular, anti-inflammatory, anti-psoriatic and antialzheimer properties. Taxifolin 3-O-rhamnoside (TAR), a glycoside of taxifolin, has antioxidant, anticonvulsant, anticancer, anti-inflammatory, and immunosuppressive effects. Objectives: The goal of this study is to separate TA and TAR from Smilax china Linn. rhizomes, as well as to develop and validate a technique for simultaneous measurement of TA and TAR. Materials and Methods: The hydro alcoholic extract of the rhizome of S. china yielded TA and TAR. HPLC system was fitted with a C 18 column (shim-pack) of size 150 mm x 4.6 mm; 5µ, with suitable eluting mixture of methanol: water (90:10 v/v) at a flowing rate of 1 ml/min and at 254 nm wavelength for identification of peaks. Lab solution software was used to establish the quantification method for above-mentioned chemical compounds. Results: TAR and TA were separated using the proposed technique at R t 2.917 and 3.924 min respectively. Over the range of 0.1-0.8 µg/ml, calibration curves were produced with a linear relationship R 2 > 0.9941 and 0.9963, respectively. The relative standard deviation was less than 2%. The percentage recoveries were shown to be between 97 and 102.1. TA and TAR had detection limits of 0.156 and 0.077 µg/ml; quantification limits were 0.473 and 0.234 µg/ml respectively. The technique that was devised was simple, sensitive and specific. Conclusion: The newly designed RP-HPLC technique has improved specificity, precision, and accuracy. The quality of S. china and other dietary supplements containing these two flavonoids may be successfully assessed by quantifying these two flavonoids and their glycosides.

Standardization of Madhumukthi Kudineer Chooranum Using Pharmacognostic, Physicochemical and HPTLC Studies

“…Both the water soluble extractives and alcohol soluble extractives were determined and found to be less than 10 % which indicates that the drug is rich in fibre and primary metabolites. In addition to the above physicochemical parameters, the principle phytochemical markers of each ingredient have to be estimated for assessing the quality of the drug by HPTLC [42] or HPLC [43] methods. The content of phytochemical markers may also be mentioned in the label claims of the drug once it is brought in the commercial market which will help in the quality control of the drug.…”