Isolation, Structure Elucidation and Antimicrobial Evaluation of Natural Pentacyclic Triterpenoids and Phytochemical Investigation of Different Fractions of Ziziphus spina-christi (L.) Stem Bark Using LCHRMS Analysis

Ads, Essam Nabih; Hassan, Syed Imran; Saravanan, R.; Hetta, Mona H.; Aly, Shaza H.; Ali, Mohamed A.

doi:10.3390/molecules27061805

Cited by 21 publications

(20 citation statements)

References 59 publications

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“…Extracts from A. gumifera, D. angustifolia, C. abyssinicum, P. abyssinica , and C. hirsuta showed moderate activity against tested microbial strains, with MIC values ranging from 10 μg/mL to 100 μg/mL, which is comparable to previous studies. 42 , 43 In contrast with some previous studies, extracts from S. nigrum, D. steudneri, T. dregaeha , and M. foetida showed insignificant activity against tested strains. 16 , 44 , 45 The difference could probably be due to differences in preparation methods, the season of plant collection, and/or environmental variations.…”

Section: Discussioncontrasting

confidence: 64%

“…The structure-activity relationship of phenol has been proven for p-hydroxy benzoic acid and different functional groups with ester side chains demonstrate excellent antibacterial activity. 10 , 43 , 48 Flavonoids are also more effective against different microbial strains than conventional medications. Naturally occurring polyphenolic chemicals distinguished by their flavan nucleus, which makes them an important component in a variety of pharmacological applications.…”

Section: Discussionmentioning

confidence: 99%

“…Therefore, the observed biological activity profile could be due to either the individual class of compounds present in each plant or the synergistic effect of each class of compounds. 38,43,49 Finally, a Kruskal-Wallis H statistical test showed that there was significant difference between the tested plant species with H (df: 11) = (X 2 :180.45, p = 0.000) and fractions of the plant extracts H (df: 3), X 2 = 7.44, p = 0.059 on MIC. But the difference in microbial strains has no significant association with the difference in MIC of the extracts.…”

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confidence: 91%

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Phytochemical Screening and Antimicrobial Activity Evaluation of Selected Medicinal Plants in Ethiopia

Dubale¹,

Kebebe²,

Zeynudin³

et al. 2023

JEP

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Background The emergence and spread of resistant microbes continue to be a major public health concern. Effective treatment alternatives, particularly from traditionally used medicinal plants, are needed. Objective The main objective of this study was to conduct phytochemical screening and antimicrobial activity evaluation of selected traditionally used medicinal plants in Ethiopia. Methods The ethnomedicinal use value frequency index (FI) was used to select twelve medicinal plants. Phytochemical classes of compounds were screened using different standard methods. Anti-microbial activities of plant extracts were evaluated against Klebsiella pneumoniae, Pseudomonas aeruginosa, Staphylococcus aureus, Escherichia coli , and Candida albicans . Minimum inhibitory concentrations were measured using the broth micro-dilution method. The data were analyzed using Statistical Package for the Social Sciences (SPSS) version 21.0 and the findings were presented descriptively and using non parametric one-way ANOVA analysis (Kruskal–Wallis/Ddunn’s test). Results The phytochemical constituents identified were flavonoids, alkaloids, glycosides, phenols, saponins, steroids, and terpenoids, with flavonoids, alkaloids, and phenols being the most abundant. The crude extracts and chloroform fractions of the extracts showed an activity against the tested strains. The crude extract of Thalictrum rhynchocarpum Quart.-Dill. and A.Rich root demonstrated superior activity against all the tested strains with the lowest minimum inhibitory concentrations of 0.48 μg/mL against Staphylococcus aureus and Escherichia coli ; 0.98 μg/mL against Klebsiella pneumoniae, Pseudomonas aeruginosa; and 3.90 μg/mL against Candida albicans , which are even better than the reference drug, gentamicin and clotrimazole. Conclusion The majority of evaluated medicinal plants demonstrated remarkable activity against tested microbial strains, which can be attributed to the presence of secondary metabolites of different classes of compounds. The finding provided scientific evidence for the use of these traditionally used medicinal plants.

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Section: Discussioncontrasting

confidence: 64%

Section: Discussionmentioning

confidence: 99%

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confidence: 91%

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Phytochemical Screening and Antimicrobial Activity Evaluation of Selected Medicinal Plants in Ethiopia

Dubale¹,

Kebebe²,

Zeynudin³

et al. 2023

JEP

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“…Information on the structure-activity relationships of active chemicals that are responsible for the killing action can be obtained by understanding the chemical components of seaweed [ 14 ]. Recently, the dependence on secondary metabolites from natural sources has been in great demand to control various ailments and defense against several conditions [ 35 , 36 , 37 , 38 , 39 , 40 ].…”

Section: Introductionmentioning

confidence: 99%

Phytochemical Investigation of Three Cystoseira Species and Their Larvicidal Activity Supported with In Silico Studies

et al. 2023

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Culex pipiens mosquitoes are transmitters of many viruses and are associated with the transmission of many diseases, such as filariasis and avian malaria, that have a high rate of mortality. The current study draws attention to the larvicidal efficacy of three methanolic algal extracts, Cystoseira myrica, C. trinodis, and C. tamariscifolia, against the third larval instar of Cx. pipiens. The UPLC-ESI-MS analysis of three methanol fractions of algal samples led to the tentative characterization of twelve compounds with different percentages among the three samples belonging to phenolics and terpenoids. Probit analysis was used to calculate the lethal concentrations (LC50 and LC90). The highest level of toxicity was attained after treatment with C. myrica extract using a lethal concentration 50 (LC50) of 105.06 ppm, followed by C. trinodis (135.08 ppm), and the lowest level of toxicity was achieved by C. tamariscifolia (138.71 ppm) after 24 h. The elevation of glutathione-S-transferase (GST) and reduction of acetylcholine esterase (AChE) enzymes confirm the larvicidal activity of the three algal extracts. When compared to untreated larvae, all evaluated extracts revealed a significant reduction in protein, lipid, and carbohydrate contents, verifying their larvicidal effectiveness. To further support the observed activity, an in silico study for the identified compounds was carried out on the two tested enzymes. Results showed that the identified compounds and the tested enzymes had excellent binding affinities for each other. Overall, the current work suggests that the three algal extractions are a prospective source for the development of innovative, environmentally friendly larvicides.

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“…Recently, there has been a great demand for the development of natural products to cure different conditions owing to their safety, availability, versatile biological activities, and unique secondary metabolites [ 27 , 28 , 29 , 30 , 31 ]. Consequently, the exploration of medicinal plants as new candidates for wound healing would be valuable and beneficial, especially medicinal plants that are characterised by biocompatibility, wound healing, and anti-inflammatory properties [ 22 , 23 , 32 , 33 ].…”

Section: Introductionmentioning

confidence: 99%

Comparative Metabolic Study of Tamarindus indica L.’s Various Organs Based on GC/MS Analysis, In Silico and In Vitro Anti-Inflammatory and Wound Healing Activities

Aly

Hassab²,

Elhady

et al. 2022

Plants

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The chemical composition of the n-hexane extract of Tamarindus indica’s various organs— bark, leaves, seeds, and fruits (TIB, TIL, TIS, TIF)—was investigated using gas chromatography-mass spectrometry (GC/MS) analysis. A total of 113 metabolites were identified, accounting for 93.07, 83.17, 84.05, and 85.08 % of the total identified components in TIB, TIL, TIS, and TIF, respectively. Lupeol was the most predominant component in TIB and TIL, accounting for 23.61 and 22.78%, respectively. However, n-Docosanoic acid (10.49%) and methyl tricosanoate (7.09%) were present in a high percentage in TIS. However, α-terpinyl acetate (7.36%) and α-muurolene (7.52%) were the major components of TIF n-hexane extract. By applying a principal component analysis (PCA) and hierarchal cluster analysis (HCA) to GC/MS-based metabolites, a clear differentiation of Tamarindus indica organs was achieved. The anti-inflammatory activity was evaluated in vitro on lipopolysaccharide (LPS)-induced RAW 264.7 macrophages. In addition, the wound healing potential for the n-hexane extract of various plant organs was assessed using the in-vitro wound scratch assay using Human Skin Fibroblast cells. The tested extracts showed considerable anti-inflammatory and wound-healing activities. At a concentration of 10 µg/mL, TIL showed the highest nitric oxide (NO) inhibition by 53.97 ± 5.89%. Regarding the wound healing potential, after 24 h, TIB, TIL, TIS, and TIF n-hexane extracts at 10 g/mL reduced the wound width to 1.09 ± 0.04, 1.12 ± 0.18, 1.09 ± 0.28, and 1.41 ± 0.35 mm, respectively, as compared to the control cells (1.37 ± 0.15 mm). These findings showed that the n-hexane extract of T. indica enhanced wound healing by promoting fibroblast migration. Additionally, a docking study was conducted to assess the major identified phytoconstituents’ affinity for binding to glycogen synthase kinase 3-β (GSK3-β), matrix metalloproteinases-8 (MMP-8), and nitric oxide synthase (iNOS). Lupeol showed the most favourable binding affinity to GSK3-β and iNOS, equal to −12.5 and −13.7 Kcal/mol, respectively, while methyl tricosanoate showed the highest binding affinity with MMP-8 equal to −13.1 Kcal/mol. Accordingly, the n-hexane extract of T. indica’s various organs can be considered a good candidate for the management of wound healing and inflammatory conditions.

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Isolation, Structure Elucidation and Antimicrobial Evaluation of Natural Pentacyclic Triterpenoids and Phytochemical Investigation of Different Fractions of Ziziphus spina-christi (L.) Stem Bark Using LCHRMS Analysis

Cited by 21 publications

References 59 publications

Phytochemical Screening and Antimicrobial Activity Evaluation of Selected Medicinal Plants in Ethiopia

Phytochemical Screening and Antimicrobial Activity Evaluation of Selected Medicinal Plants in Ethiopia

Phytochemical Investigation of Three Cystoseira Species and Their Larvicidal Activity Supported with In Silico Studies

Comparative Metabolic Study of Tamarindus indica L.’s Various Organs Based on GC/MS Analysis, In Silico and In Vitro Anti-Inflammatory and Wound Healing Activities

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