Isolation, structure elucidation, and biological activity of polysaccharides from Saussurea involucrata

Liu, Guangrong; Kamilijiang, Mayila; Abuduwaili, Aytursun; Zang, Deng; Abudukelimu, Nuermaimaiti; Liu, Geyu; Yili, Abulimiti; Aisa, Haji Akber

doi:10.1016/j.ijbiomac.2022.09.137

Cited by 32 publications

(19 citation statements)

References 43 publications

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“…For instance, polysaccharides consisting of α-glucose or α-mannose exhibited powerful immunomodulatory activity on macrophages when the polysaccharides were recognized by TLR4 or TLR2 receptors. 58 Mannosan was isolated from Bacillus sp. H5 with a backbone structure of →1)-α-D-Manp-(4→, →1)-α-D-Manp-(2→, and →1,4)-α-D-Manp-(6→ and branch structure of β-D-Manp-(1→ enhanced the immune regulatory effect of macrophages by energizing the NF-κB pathway and activating the TLR4 receptor.…”

Section: ■ Discussionmentioning

confidence: 99%

“…Notably, the immune regulatory effect of a polysaccharide is inevitably related to the composition and type of sugar residues. For instance, polysaccharides consisting of α-glucose or α-mannose exhibited powerful immunomodulatory activity on macrophages when the polysaccharides were recognized by TLR4 or TLR2 receptors . Mannosan was isolated from Bacillus sp.…”

Section: Discussionmentioning

confidence: 99%

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Structural Characterization and Immunomodulatory Activity of a Mannose-Rich Polysaccharide Isolated from Bifidobacterium breve H4–2

Niu,

Guo,

Shang

et al. 2023

J. Agric. Food Chem.

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Section: ■ Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Structural Characterization and Immunomodulatory Activity of a Mannose-Rich Polysaccharide Isolated from Bifidobacterium breve H4–2

Niu,

Guo,

Shang

et al. 2023

J. Agric. Food Chem.

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“…S. involucrata is a dicotyledonous plant used as a traditional medicinal plant in ethnomedicine that was discovered to have several bioactivities including anti-cancer, anti-inflammation, anti-oxidation, adipogenesis suppression, and neuroprotection activities [ 34 ]. Our previous research showed that polysaccharides from S. involucrata possessed remarkable anti-inflammatory activity indicating their potential in the functional food, pharmaceutical, and cosmetical industries [ 41 ]. To our knowledge, the investigation of the cosmetic application of SIP is rare.…”

Section: Discussionmentioning

confidence: 99%

“…S. involucrata polysaccharide (SIP) was prepared in our prior research and preserved at the State Key Laboratory Basis of Xinjiang Indigenous Medicinal Plants Resource Utilization. The methods for separation and analysis were described by Liu et al [ 41 ]. Briefly, the S. involucrata was dried and defatted with petroleum ether (1:10, w / v ) 3 times for 2 h followed by air-drying and de-coloring.…”

Section: Methodsmentioning

confidence: 99%

“…The bioactive components found in S. involucrata , including flavones, phenylpropanoids, polysaccharides, lignans, and sesquiterpenoids, were linked to some bioactivities, including antioxidant, anti-UV, and anti-aging [ 35 , 36 , 37 , 38 , 39 , 40 ]. In a previous study, we isolated the polysaccharide from S. involucrata and found that it possessed great anti-inflammatory activity, indicating its potential use in the functional food, pharmaceutical, and cosmetical industry [ 41 ].…”

Section: Introductionmentioning

confidence: 99%

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Anti-Melanogenesis Effect of Polysaccharide from Saussurea involucrata on Forskolin-Induced Melanogenesis in B16F10 Melanoma Cells

et al. 2022

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As one of the prominent medicinal plants listed in the Chinese pharmacopoeia (2020), Saussurea involucrata (Kar. et Kir.) Sch.-Bip was demonstrated to possess various therapeutic effects. In our recent research, we extracted the polysaccharides from S. involucrata (SIP) at optimal conditions and conducted further structure elucidation on the main fraction as well as the confirmation of its possible anti-inflammatory activity. Hence, in this work, we assessed the in vitro antioxidant activity and anti-melanogenesis effects of the crude SIP in forskolin-induced B16F10 melanoma cells. The results show that SIP possessed strong antioxidant activity and was effective in concentration-dependently decreasing melanin formation and inhibiting tyrosinase activity in forskolin-induced B16F10 cells. Based on these results, the inhibitory mechanism of melanogenesis was investigated by measuring Tyrosinase (TYR), Tyrosinase related protein-1 (TRP-1), Tyrosinase related protein-2 (TRP-2), Microphthalmia-associated transcription factor (MITF), cAMP-response element binding protein (CREB), mitogen-activated protein kinases (MAPK) signaling protein members, and β-catenin degradation in forskolin-induced B16F10 cells. The anti-melanogenesis response of SIP might be attributed to the regulation of c-Jun N-terminal kinase (JNK) phosphorylation and β-catenin degradation pathways. These results suggest that polysaccharides from S. involucrata possess a strong anti-melanogenic effect, and thus could be used as a high-value natural material for skin whitening in cosmeceutical industries.

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Effects of Separation and Purification Methods on Antioxidation, Hypoglycemic and DNA Protection Activity of Fenugreek Polysaccharide

Wang,

Feng,

Liang

et al. 2024

Chemistry & Biodiversity

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Six low molecular weight fenugreek polysaccharides (FP) were isolated and purified by ethanol stepwise precipitation (EFP‐20, EFP‐40, and EFP‐60) and DEAE‐52 cellulose column method (DFP‐0, DFP‐0.15, and DFP‐0.3), respectively. The effects of different separation and purification techniques on the preliminary properties and biological activities of fenugreek polysaccharides were compared. The results showed that the DEAE‐52 cellulose‐eluted fractions had a higher total sugar content and displayed a looser structure. The molecular weights of all six fractions were in the range of 4‐19 kDa, with significant changes in the ratio of galactose to mannose. All six fractions contained α‐D‐galactopyranose and β‐D‐mannopyranose structures. Activity tests showed that all six fractions possessed antioxidant, hypoglycemic and DNA‐protective activities. Among them, the DFP‐0 fraction showed the highest activity. Overall, different isolation and purification methods lead to changes in the properties and bioactivities of FP, which provides a theoretical basis for the development and application of FP in functional foods and drugs.

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Isolation, structure elucidation, and biological activity of polysaccharides from Saussurea involucrata

Cited by 32 publications

References 43 publications

Structural Characterization and Immunomodulatory Activity of a Mannose-Rich Polysaccharide Isolated from Bifidobacterium breve H4–2

Structural Characterization and Immunomodulatory Activity of a Mannose-Rich Polysaccharide Isolated from Bifidobacterium breve H4–2

Anti-Melanogenesis Effect of Polysaccharide from Saussurea involucrata on Forskolin-Induced Melanogenesis in B16F10 Melanoma Cells

Effects of Separation and Purification Methods on Antioxidation, Hypoglycemic and DNA Protection Activity of Fenugreek Polysaccharide

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