Isoniazid Induced Liver Damage;

Abstract

April to September 2013. Methods: Total 28 rabbits of weight 1-1.5kg of either sex were used in this study. Which were divided randomly into four equal groups: Group I was control group. In group II silymarin (50mg/kg/day orally) was administered, in group III isoniazid (50mg/kg/dayorally) was given; and in group IV, effects of combination therapy of isoniazid and silymarin were observed. Before starting the drug therapy, at day 0 and one day after the end of study period i.e., at day 19, body weight of each animal was recorded. Rabbits were sacrificed on 19 th day and the required liver sample was taken for histopathological examination. The data feeding and analysis at the end of study was done on computer package SPSS (Statistical packages of social science) version 16. Results: No mortality was recorded in any group. In group II (silymarin treated) animals in this group exhibited no any histological changes in the hepatic lobule except few inflammatory cells 28.5% were seen in the portal tract. The liver microscopic examination in group III (Isoniazid treated), animals showed the disturbed architecture of the lobule. There were no fatty changes, whereas ballooning degeneration was 42.9%, hepatocytes necrosis was 71% and portal inflammation was 71.4% which was very severe. Animals in group IV, given combination of silymarin and isoniazid showed the intact architecture of the hepatic lobule, in which 14.29% ballooning degeneration, whereas necrosis of hepatocytes and portal inflammation was mild in nature which may be due to hepatoprotective role of silymarin. Conclusion: Silymarin has hepatoprotective effects when given in combination with isoniazid.

Use of Milk Thistle in Farm and Companion Animals: A Review

“…Another study focused on the protective effects of SIL after the application of drugs with side effects causing hepatotoxicity. Jahan and colleagues [83] tested the effects of SIL in rabbits experimentally poisoned with isoniazid at a dose of 50 mg/kg once daily orally. At the same dose and pathway, SIL (composition not reported) was administered.…”

Comparison of hepatoprotective effect of aqueous and ethanolic extracts of Berberis lycium Royale (Sumbloo) in isoniazid induced hepatotoxicity in male mice model

“…7 Hepatotoxicity produced by INH is proven by observing inflammatory infiltrates, steatosis and hepatocytic ballooning. 8,9 Previous research studies have shown hepatoprotective effect of extracts of Berberis lycium Royle in combination with other herbs. 10 No research study has been carried out as yet on comparison of aqueous and ethanolic extract of Berberis lycium Royle in perspective of hepatoprotective effect.…”