Isospora lunaris n. sp. (Apicomplexa: Eimeriidae) from the domestic Java sparrow in Japan

Tokiwa, Toshihiro; Kojima, Akira; Shun, Sasaki; Kubota, Rie; Ike, Kazunori

doi:10.1016/j.parint.2016.12.007

Cited by 5 publications

(1 citation statement)

References 22 publications

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“…Oocysts were purified by flotation methods from each specimens separately, and a single oocyst was isolated using a manually pulled glass micropipette under an Olympus SZX stereomicroscope (Olympus). Genomic DNA was extracted using a Power Soil DNA isolation kit (MoBio Laboratories, USA) as previously described ( Tokiwa et al, 2017 ). Double distilled water (no fecal specimen) was included as a negative control.…”

Section: Methodsmentioning

confidence: 99%

Morphological and molecular characterization of Cystoisospora sp. from Asian small-clawed otters Aonyx cinereus

Tokiwa

Ohnuki

Kubota

et al. 2018

International Journal for Parasitology: Parasites and Wildlife

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In this study, we provide the first description of Cystoisospora infection in Asian small-clawed otters (Aonyx cinereus). In July 2017, three juvenile otters recently imported from the Republic of Indonesia showed severe diarrhea and were diagnosed with coccidial infection; two of them eventually died. Fecal examination revealed the presence of numerous oocysts. Sporulated oocysts showed typical Cystoisospora features, measuring 24.6 ± 1.6 (22.0–27.0) × 21.8 ± 1.4 (19.0–25.0) μm, with an oocyst length/width ratio of 1.1 ± 0.1 (1.0–1.3). Each sporocyst contained four sporozoites in a head-to-tail arrangement. The Stieda body was absent, and the sporocyst residuum was present. These morphological characteristics differentiated this species from the other valid Cystoisospora species described from mustelids. Molecular analysis was conducted at two loci: the nuclear 18S ribosomal RNA and mitochondrial cytochrome c oxidase subunit I genes. The 18S sequence showed high similarity with canine Cystoisispora ohioensis (1-bp difference, 1422/1423 [99.9%]). At the cytochrome c oxidase subunit I gene locus, the sequence from otters was identical to that of feline Cystoisospora rivolta (847/847 [100%]). Phylogenetic analyses using concatenated data demonstrated that Cystoisospora sp. from otters and C. rivolta grouped together in the same Cystoisospora clade. Based on these data, we concluded that Cystoisospora sp. detected from otters appeared to be highly similar to C. rivolta.

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Section: Methodsmentioning

confidence: 99%

Morphological and molecular characterization of Cystoisospora sp. from Asian small-clawed otters Aonyx cinereus

Tokiwa

Ohnuki

Kubota

et al. 2018

International Journal for Parasitology: Parasites and Wildlife

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Detection and characterization of the Isospora lunaris infection from different finch hosts in southern Iran

et al. 2020

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Isospora lopesi n. sp. (Apicomplexa: Eimeriidae) from the eastern white-throated spadebill Platyrinchus mystaceus Vieillot (Passeriformes: Tyranni: Tyrannidae) in South America

et al. 2018

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A species of Isospora Schneider, 1881 (Protozoa: Apicomplexa: Eimeriidae) considered as new to science is described and characterised molecularly from the eastern white-throated spadebill Platyrinchus mystaceus Vieillot in the Parque Nacional do Itatiaia, southeastern Brazil. Isospora lopesi n. sp. has oöcysts that are subspheroidal to ovoidal, 18-24 × 18-22 (20.6 × 19.7) µm, with smooth, bilayered wall, c.1.5 μm thick. Micropyle and oöcyst residuum are absent, but one polar granule is present. Sporocysts are ellipsoidal, 12-16 × 8-11 (14.4 × 8.6) µm. The Stieda body is flattened to half-moon-shaped and sub-Stieda body is rounded. Sporocyst residuum is present, consisting of numerous spherules of different sizes. Sporozoites are vermiform with anterior and posterior refractile bodies and nucleus. Molecular analysis was conducted at the mitochondrial cytochrome c oxidase subunit 1 (cox1) gene. This new isolate exhibited similarity greater than 98% with Isospora spp. isolates from spectacled warblers Sylvia conspicillata Temminck, 1820. This is the fourth isosporoid coccidian described from New World tyrannid birds, but is the first to have a complementary molecular characterisation.

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Isospora lunaris n. sp. (Apicomplexa: Eimeriidae) from the domestic Java sparrow in Japan

Cited by 5 publications

References 22 publications

Morphological and molecular characterization of Cystoisospora sp. from Asian small-clawed otters Aonyx cinereus

Morphological and molecular characterization of Cystoisospora sp. from Asian small-clawed otters Aonyx cinereus

Detection and characterization of the Isospora lunaris infection from different finch hosts in southern Iran

Isospora lopesi n. sp. (Apicomplexa: Eimeriidae) from the eastern white-throated spadebill Platyrinchus mystaceus Vieillot (Passeriformes: Tyranni: Tyrannidae) in South America

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